PMID- 35163164
OWN - NLM
STAT- MEDLINE
DCOM- 20220304
LR  - 20220304
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 3
DP  - 2022 Jan 22
TI  - Role of Periostin Expression in Non-Small Cell Lung Cancer: Periostin Silencing 
      Inhibits the Migration and Invasion of Lung Cancer Cells via Regulation of MMP-2 
      Expression.
LID - 10.3390/ijms23031240 [doi]
LID - 1240
AB  - The involvement of periostin (POSTN) in non-small-cell lung cancer (NSCLC) 
      migration, invasion, and its underlying mechanisms has not been well established. 
      The present study aims to determine epithelial POSTN expression in NSCLC and to 
      assess associations with clinicopathological factors and prognosis as well as to 
      explore the effects of POSTN knockdown on tumor microenvironment and the 
      migration and invasion of lung cancer cells. Immunohistochemistry was used to 
      evaluate epithelial POSTN expression in NSCLC. POSTN mRNA expression in the 
      dissected lung cancer cells was confirmed by laser capture microdissection and 
      real-time PCR. A549 cells were used for transfecting shRNA-POSTN lentiviral 
      particles. Wound healing and Transwell invasion assays were used to assess the 
      migratory and invasive abilities of A549 cells transfected with POSTN-specific 
      short hairpin (sh)RNA. The results demonstrated significantly higher cytoplasmic 
      POSTN expression in the whole NSCLC group compared to non-malignant lung tissue 
      (NMLT). POSTN expression in cancer cells may be considered to be an independent 
      prognostic factor for survival in NSCLC. POSTN knockdown significantly inhibited 
      A549 cell migration and invasion capabilities in vitro. The activity and the 
      expression level of matrix metalloproteinase-2 (MMP-2) were significantly 
      decreased in A549.shRNA compared to control cells. In summary, POSTN may regulate 
      lung cancer cell invasiveness by modulating the expression of MMP-2 and may 
      represent a potential target for novel therapeutic intervention for NSCLC.
FAU - Ratajczak-Wielgomas, Katarzyna
AU  - Ratajczak-Wielgomas K
AUID- ORCID: 0000-0001-6175-2204
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
FAU - Kmiecik, Alicja
AU  - Kmiecik A
AUID- ORCID: 0000-0002-6498-717X
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
FAU - Dziegiel, Piotr
AU  - Dziegiel P
AUID- ORCID: 0000-0002-8292-1385
AD  - Division of Histology and Embryology, Department of Human Morphology and 
      Embryology, Wroclaw Medical University, 50-368 Wroclaw, Poland.
AD  - Department of Human Biology, Faculty of Physiotherapy, University School of 
      Physical Education, 51-612 Wroclaw, Poland.
LA  - eng
PT  - Journal Article
DEP - 20220122
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (POSTN protein, human)
RN  - EC 3.4.24.24 (MMP2 protein, human)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Apoptosis
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Carcinoma, Non-Small-Cell Lung/genetics/metabolism/*pathology
MH  - Cell Adhesion Molecules/antagonists & inhibitors/genetics/*metabolism
MH  - *Cell Movement
MH  - Cell Proliferation
MH  - Epithelial-Mesenchymal Transition
MH  - *Gene Expression Regulation, Neoplastic
MH  - Gene Silencing
MH  - Humans
MH  - Lung Neoplasms/genetics/metabolism/*pathology
MH  - Matrix Metalloproteinase 2/genetics/*metabolism
MH  - Neoplasm Invasiveness
MH  - Prognosis
MH  - Survival Rate
MH  - Tumor Cells, Cultured
MH  - Tumor Microenvironment
PMC - PMC8835752
OTO - NOTNLM
OT  - extracellular matrix
OT  - invasion
OT  - matrix metalloproteinase-2
OT  - non-small cell lung carcinoma
OT  - periostin
OT  - remodeling
COIS- The authors declare no conflict of interest.
EDAT- 2022/02/16 06:00
MHDA- 2022/03/05 06:00
PMCR- 2022/01/22
CRDT- 2022/02/15 01:16
PHST- 2021/12/14 00:00 [received]
PHST- 2022/01/18 00:00 [revised]
PHST- 2022/01/20 00:00 [accepted]
PHST- 2022/02/15 01:16 [entrez]
PHST- 2022/02/16 06:00 [pubmed]
PHST- 2022/03/05 06:00 [medline]
PHST- 2022/01/22 00:00 [pmc-release]
AID - ijms23031240 [pii]
AID - ijms-23-01240 [pii]
AID - 10.3390/ijms23031240 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Jan 22;23(3):1240. doi: 10.3390/ijms23031240.