PMID- 35163257
OWN - NLM
STAT- MEDLINE
DCOM- 20220309
LR  - 20220309
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 3
DP  - 2022 Jan 25
TI  - Expression of Doublecortin, Glial Fibrillar Acidic Protein, and Vimentin in the 
      Intact Subpallium and after Traumatic Injury to the Pallium in Juvenile Salmon, 
      Oncorhynchus masou.
LID - 10.3390/ijms23031334 [doi]
LID - 1334
AB  - Fetalization associated with a delay in development and the preservation of the 
      features of the embryonic structure of the brain dominates the ontogeny of 
      salmonids. The aim of the present study was to comparatively analyze the 
      distribution of the glial-type aNSC markers such as vimentin and glial fibrillar 
      acidic protein (GFAP) and the migratory neuronal precursors such as doublecortin 
      in the telencephalon subpallium of juvenile masu salmon, Oncorhynchus masou, in 
      normal conditions and at 1 week after an injury to the dorsal pallium. 
      Immunohistochemical labeling of vimentin, GFAP, and doublecortin in the pallium 
      of intact juvenile masu salmon revealed single cells with similar morphologies 
      corresponding to a persistent pool of neuronal and/or glial progenitors. The 
      study of the posttraumatic process showed the presence of intensely GFAP-labeled 
      cells of the neuroepithelial type that form reactive neurogenic zones in all 
      areas of the subpallial zone of juvenile masu salmon. A comparative analysis of 
      the distribution of radial glia in the dorsal, ventral, and lateral zones of the 
      subpallium showed a maximum concentration of cells in the dorsal part of 
      subpallium (VD) and a minimum concentration in the lateral part of subpallium VL. 
      An essential feature of posttraumatic immunolabeling in the masu salmon 
      subpallium is the GFAP distribution patterns that are granular intracellular in 
      the apical periventricular zone (PVZ) and fibrillar extracellular in the 
      subventricular (SVZ) and parenchymal zones (PZ). In contrast to those in intact 
      animals, most of the GFAP+ granules and constitutive neurogenic niches in injured 
      fish were localized in the basal part of the PVZ. With the traumatic injury to 
      the subpallium, the number of Vim+ cells in the lateral and ventral regions 
      significantly increased. At 1 week post-injury, the total immunolabeling of 
      vimentin cells in the PVZ was replaced by the granular pattern of Vim 
      immunodistribution spreading from the PVZ to the SVZ and deeper parenchymal 
      layers of the brain in all areas of the subpallium. A significant increase in the 
      number of DC+ cells was observed also in all areas of the subpallium. The number 
      of cells increased both in the PVZ and in the SVZ, as well as in the deeper PZ. 
      Thus, at 1 week after the injury to the dorsal pallium, the number of DC, Vim, 
      and GFAP expressing cells of the neuroepithelial type in the subpallium of 
      juvenile masu salmon increased, and additionally GFAP+ radial glia appeared in 
      VD, which was absent from intact animals.
FAU - Pushchina, Evgeniya V
AU  - Pushchina EV
AUID- ORCID: 0000-0003-0388-3147
AD  - A.V. Zhirmunsky National Scientific Center of Marine Biology, Far East Branch, 
      Russian Academy of Sciences, 690041 Vladivostok, Russia.
FAU - Zharikova, Eva I
AU  - Zharikova EI
AD  - A.V. Zhirmunsky National Scientific Center of Marine Biology, Far East Branch, 
      Russian Academy of Sciences, 690041 Vladivostok, Russia.
FAU - Varaksin, Anatoly A
AU  - Varaksin AA
AD  - A.V. Zhirmunsky National Scientific Center of Marine Biology, Far East Branch, 
      Russian Academy of Sciences, 690041 Vladivostok, Russia.
LA  - eng
GR  - 20-34-90091/Russian Foundation for Basic Research/
PT  - Journal Article
DEP - 20220125
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Doublecortin Domain Proteins)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Vimentin)
SB  - IM
MH  - Animals
MH  - Brain Injuries, Traumatic/*metabolism
MH  - Cerebral Cortex/*metabolism
MH  - Doublecortin Domain Proteins/*metabolism
MH  - Ependymoglial Cells/metabolism
MH  - Glial Fibrillary Acidic Protein/*metabolism
MH  - Intermediate Filaments
MH  - Neurogenesis/physiology
MH  - Neuroglia/metabolism
MH  - Neurons/metabolism
MH  - Oncorhynchus/*metabolism
MH  - Salmon/*metabolism
MH  - Telencephalon/metabolism
MH  - Vimentin/*metabolism
PMC - PMC8836249
OTO - NOTNLM
OT  - GFAP
OT  - adult neuronal stem cells
OT  - doublecortin
OT  - masu salmon
OT  - neuronal precursor cells
OT  - post-mitotic neuroblasts
OT  - proliferation of cells
OT  - subpallial part of telencephalon
OT  - traumatic brain injury
OT  - vimentin
COIS- The authors declare no conflict of interest.
EDAT- 2022/02/16 06:00
MHDA- 2022/03/11 06:00
PMCR- 2022/01/25
CRDT- 2022/02/15 01:16
PHST- 2021/12/24 00:00 [received]
PHST- 2022/01/17 00:00 [revised]
PHST- 2022/01/22 00:00 [accepted]
PHST- 2022/02/15 01:16 [entrez]
PHST- 2022/02/16 06:00 [pubmed]
PHST- 2022/03/11 06:00 [medline]
PHST- 2022/01/25 00:00 [pmc-release]
AID - ijms23031334 [pii]
AID - ijms-23-01334 [pii]
AID - 10.3390/ijms23031334 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Jan 25;23(3):1334. doi: 10.3390/ijms23031334.