PMID- 35163877
OWN - NLM
STAT- MEDLINE
DCOM- 20220228
LR  - 20220228
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 27
IP  - 3
DP  - 2022 Jan 18
TI  - Effect of Extraction Procedures with Ultrasound and Cellulolytic Enzymes on the 
      Structural and Functional Properties of Citrus grandis Osbeck Seed Mucilage.
LID - 10.3390/molecules27030612 [doi]
LID - 612
AB  - The structural and functional properties of Citrus grandis Osbeck (CGO) seed 
      mucilage by different extraction practices, including conventional citrate 
      buffer, ultrasonic-assisted (UAE), enzymatic-assisted extraction (EAE) with 
      cellulase or Celluclast((R)) 1.5 L and various ultrasonic-assisted enzymatic 
      extraction (UAEE) procedures were investigated. It was found that CGO seed from 
      agricultural and processing byproducts is an excellent new source of high 
      methoxyl pectin with quite high intrinsic viscosity (about 108.64 dL/g) and 
      molecular weight (about 1.9 x 10(6)) as compared with other pectin sources. UAEE 
      with Celluclast((R)) 1.5 L enhanced the extraction yield most pronouncedly (about 
      2.3 times). Moreover, the monosaccharide composition of CGO seed mucilage is 
      least affected by EAE with Celluclast((R)) 1.5 L. In contrast, EAE with cellulase 
      dramatically reduces the galacturonic acid (GalA) content to less than 60 molar%, 
      and increases the glucose (Glc) content pronouncedly (to about 40 molar%), which 
      may be considered as an adverse effect in terms of pectin purity. Though 
      extraction procedures involved with ultrasound and cellulolytic enzymes generally 
      show a decrease in GalA contents, weight average molar mass and intrinsic 
      viscosity, EAE with Celluclast((R)) 1.5 L is least affected, followed by UAE and 
      UAEE with Celluclast((R)) 1.5 L. These features can be leveraged in favor of 
      diversified applications.
FAU - Yeh, Yu-Cheng
AU  - Yeh YC
AD  - Department of Food Science and Biotechnology, National Chung Hsing University, 
      145 Xingda Road, Taichung 40227, Taiwan.
FAU - Lai, Lih-Shiuh
AU  - Lai LS
AUID- ORCID: 0000-0002-5741-1084
AD  - Department of Food Science and Biotechnology, National Chung Hsing University, 
      145 Xingda Road, Taichung 40227, Taiwan.
LA  - eng
GR  - MOST 106-2320-B-005-007-MY2/Ministry of Science and Technology (MOST) in Taiwan/
PT  - Journal Article
DEP - 20220118
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Hexuronic Acids)
RN  - 0 (Plant Extracts)
RN  - 4JK6RN80GF (galacturonic acid)
RN  - EC 3.2.1.4 (Cellulase)
SB  - IM
MH  - Cellulase/*metabolism
MH  - Citrus/*chemistry/*metabolism/radiation effects
MH  - Hexuronic Acids/*metabolism
MH  - Plant Extracts/*isolation & purification
MH  - Seeds/*chemistry/*metabolism/radiation effects
MH  - *Ultrasonic Waves
PMC - PMC8838855
OTO - NOTNLM
OT  - enzyme-assisted extraction (EAE)
OT  - seed mucilage
OT  - ultrasonic-assisted enzymatic extraction (UAEE)
OT  - ultrasound-assisted extraction (UAE)
COIS- The authors declare no conflict of interest.
EDAT- 2022/02/16 06:00
MHDA- 2022/03/01 06:00
PMCR- 2022/01/18
CRDT- 2022/02/15 01:19
PHST- 2021/11/30 00:00 [received]
PHST- 2022/01/14 00:00 [revised]
PHST- 2022/01/17 00:00 [accepted]
PHST- 2022/02/15 01:19 [entrez]
PHST- 2022/02/16 06:00 [pubmed]
PHST- 2022/03/01 06:00 [medline]
PHST- 2022/01/18 00:00 [pmc-release]
AID - molecules27030612 [pii]
AID - molecules-27-00612 [pii]
AID - 10.3390/molecules27030612 [doi]
PST - epublish
SO  - Molecules. 2022 Jan 18;27(3):612. doi: 10.3390/molecules27030612.