PMID- 35164031
OWN - NLM
STAT- MEDLINE
DCOM- 20220225
LR  - 20220225
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 27
IP  - 3
DP  - 2022 Jan 25
TI  - Anti-Fibrotic Effect of Synthetic Noncoding Oligodeoxynucleotide for Inhibiting 
      mTOR and STAT3 via the Regulation of Autophagy in an Animal Model of Renal 
      Injury.
LID - 10.3390/molecules27030766 [doi]
LID - 766
AB  - Renal fibrosis is a common process of various kidney diseases. Autophagy is an 
      important cell biology process to maintain cellular homeostasis. In addition, 
      autophagy is involved in the pathogenesis of various renal disease, including 
      acute kidney injury, glomerular diseases, and renal fibrosis. However, the 
      functional role of autophagy in renal fibrosis remains poorly unclear. The 
      mammalian target of rapamycin (mTOR) plays a negative regulatory role in 
      autophagy. Signal transducer and activator of transcription 3 (STAT3) is an 
      important intracellular signaling that may regulate a variety of inflammatory 
      responses. In addition, STAT3 regulates autophagy in various cell types. Thus, we 
      synthesized the mTOR/STAT3 oligodeoxynucleotide (ODN) to regulate the autophagy. 
      The aim of this study was to investigate the beneficial effect of mTOR/STAT3 ODN 
      via the regulation of autophagy appearance on unilateral ureteral obstruction 
      (UUO)-induced renal fibrosis. This study showed that UUO induced inflammation, 
      tubular atrophy, and tubular interstitial fibrosis. However, mTOR/STAT3 ODN 
      suppressed UUO-induced renal fibrosis and inflammation. The autophagy markers 
      have no statistically significant relation, whereas mTOR/STAT3 ODN suppressed the 
      apoptosis in tubular cells. These results suggest the possibility of mTOR/STAT3 
      ODN for preventing renal fibrosis. However, the role of mTOR/STAT3 ODN on 
      autophagy regulation needs to be further investigated.
FAU - Jung, Hyun Jin
AU  - Jung HJ
AD  - Department of Urology, College of Medicine, Catholic University of Daegu, Daegu 
      42472, Korea.
FAU - An, Hyun-Jin
AU  - An HJ
AD  - Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu 
      42472, Korea.
FAU - Gwon, Mi-Gyeong
AU  - Gwon MG
AD  - Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu 
      42472, Korea.
FAU - Gu, Hyemin
AU  - Gu H
AD  - Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu 
      42472, Korea.
FAU - Bae, Seongjae
AU  - Bae S
AD  - Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu 
      42472, Korea.
FAU - Lee, Sun-Jae
AU  - Lee SJ
AUID- ORCID: 0000-0002-8552-6049
AD  - Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu 
      42472, Korea.
FAU - Kim, Young-Ah
AU  - Kim YA
AUID- ORCID: 0000-0001-7850-5509
AD  - Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu 
      42472, Korea.
FAU - Leem, Jaechan
AU  - Leem J
AUID- ORCID: 0000-0003-2329-4374
AD  - Department of Immunology, College of Medicine, Catholic University of Daegu, 
      Daegu 42472, Korea.
FAU - Park, Kwan-Kyu
AU  - Park KK
AD  - Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu 
      42472, Korea.
LA  - eng
GR  - NRF-2019R1G1A1010882/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20220125
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (STAT3 Transcription Factor)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Autophagy/*drug effects
MH  - Disease Models, Animal
MH  - Fibrosis/*prevention & control
MH  - Kidney/*injuries
MH  - Oligodeoxyribonucleotides/*antagonists & inhibitors
MH  - STAT3 Transcription Factor/*metabolism
MH  - TOR Serine-Threonine Kinases/*metabolism
PMC - PMC8840279
OTO - NOTNLM
OT  - STAT3
OT  - antisense
OT  - autophagy
OT  - decoy
OT  - mTOR
OT  - oligodeoxynucleotide
OT  - renal fibrosis
COIS- The authors declare no conflict of interest.
EDAT- 2022/02/16 06:00
MHDA- 2022/02/26 06:00
PMCR- 2022/01/25
CRDT- 2022/02/15 01:19
PHST- 2021/12/14 00:00 [received]
PHST- 2022/01/10 00:00 [revised]
PHST- 2022/01/19 00:00 [accepted]
PHST- 2022/02/15 01:19 [entrez]
PHST- 2022/02/16 06:00 [pubmed]
PHST- 2022/02/26 06:00 [medline]
PHST- 2022/01/25 00:00 [pmc-release]
AID - molecules27030766 [pii]
AID - molecules-27-00766 [pii]
AID - 10.3390/molecules27030766 [doi]
PST - epublish
SO  - Molecules. 2022 Jan 25;27(3):766. doi: 10.3390/molecules27030766.