PMID- 35166175
OWN - NLM
STAT- MEDLINE
DCOM- 20220325
LR  - 20220403
IS  - 2165-5987 (Electronic)
IS  - 2165-5979 (Print)
IS  - 2165-5979 (Linking)
VI  - 13
IP  - 2
DP  - 2022 Feb
TI  - Astragalus polysaccharide (APS) exerts protective effect against acute ischemic 
      stroke (AIS) through enhancing M2 micoglia polarization by regulating adenosine 
      triphosphate (ATP)/ purinergic receptor (P2X7R) axis.
PG  - 4468-4480
LID - 10.1080/21655979.2021.1980176 [doi]
AB  - Clinically, the effective treatment for patients with acute ischemic stroke (AIS) 
      is very limited. Therefore, this paper aims to investigate the mechanism how 
      astragalus polysaccharide (APS) exerts protective effect against AIS and provide 
      a new method for the treatment of AIS. Cell surface antigen flow cytometry and 
      immunofluorescence were used to identify M1 and M2 microglia. Western blot was 
      used to evaluate the expression of associated protein. Oxygen-glucose deprivation 
      (OGD) was used to simulate the effect of AIS on rat microglia. The middle 
      cerebral artery occlusion (MCAO) model was established to simulate the effect of 
      AIS in vivo. Evans blue dye (EBD) was used to evaluate the permeability of 
      blood-brain barrier (BBB). Western blot and cell surface antigen flow cytometry 
      results showed that APS promoted the M2 polarization of rat microglia by 
      inhibiting the expression of purinergic receptor (P2X7R). APS reversed the effect 
      of OGD on the polarization of rat microglia M1/ M2 by regulating P2X7R. APS 
      reversed the effect of MCAO on the polarization of rat microglia M1/ M2 in vivo. 
      Furthermore, APS inhibited the expression of P2X7R by promoting the degradation 
      of adenosine triphosphate (ATP) in the cerebral cortex of MCAO rats. In addition, 
      APS contributed to maintain the integrity of BBB. Summarily, APS can reduce brain 
      injury by promoting the degradation of ATP in microglia and inhibiting the 
      expression of P2X7R after AIS.
FAU - Jia, Xiang
AU  - Jia X
AD  - Department of Neurology, Guangzhou Hospital of Integrated Traditional and West 
      Medicine, Guangzhou, China.
FAU - Xie, Liying
AU  - Xie L
AD  - Department of Neurology, Guangzhou Hospital of Integrated Traditional and West 
      Medicine, Guangzhou, China.
FAU - Liu, Yuan
AU  - Liu Y
AD  - Department of Neurology, Guangzhou Hospital of Integrated Traditional and West 
      Medicine, Guangzhou, China.
FAU - Liu, Tianfu
AU  - Liu T
AD  - Department of Neurology, Guangzhou Hospital of Integrated Traditional and West 
      Medicine, Guangzhou, China.
FAU - Yang, Peiqun
AU  - Yang P
AD  - Department of Neurology, Guangzhou Hospital of Integrated Traditional and West 
      Medicine, Guangzhou, China.
FAU - Hu, Jianfang
AU  - Hu J
AD  - Department of Neurology, Guangzhou Hospital of Integrated Traditional and West 
      Medicine, Guangzhou, China.
FAU - Peng, Zhichao
AU  - Peng Z
AD  - Department of Neurology, Guangzhou Hospital of Integrated Traditional and West 
      Medicine, Guangzhou, China.
FAU - Luo, Kangrui
AU  - Luo K
AD  - Department of Neurology, Guangzhou Hospital of Integrated Traditional and West 
      Medicine, Guangzhou, China.
FAU - Du, Min
AU  - Du M
AD  - Nursing Department, Guangzhou Hospital of Integrated Traditional and West 
      Medicine, Guangzhou, China.
FAU - Chen, Chaojun
AU  - Chen C
AD  - Department of Neurology, Guangzhou Hospital of Integrated Traditional and West 
      Medicine, Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Bioengineered
JT  - Bioengineered
JID - 101581063
RN  - 0 (Polysaccharides)
RN  - 0 (Protective Agents)
RN  - 0 (Receptors, Purinergic P2X7)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - *Astragalus Plant
MH  - Blood-Brain Barrier/drug effects
MH  - Cell Line
MH  - Infarction, Middle Cerebral Artery/metabolism
MH  - Ischemic Stroke/*metabolism
MH  - Male
MH  - *Microglia/cytology/drug effects
MH  - *Polysaccharides/chemistry/pharmacology
MH  - *Protective Agents/chemistry/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Purinergic P2X7/metabolism
PMC - PMC8973874
OTO - NOTNLM
OT  - Acute ischemic stroke
OT  - astragalus polysaccharide
OT  - blood-brain barrier
OT  - microglia
OT  - purinergic receptor
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2022/02/16 06:00
MHDA- 2022/03/26 06:00
PMCR- 2022/02/15
CRDT- 2022/02/15 08:52
PHST- 2022/02/15 08:52 [entrez]
PHST- 2022/02/16 06:00 [pubmed]
PHST- 2022/03/26 06:00 [medline]
PHST- 2022/02/15 00:00 [pmc-release]
AID - 1980176 [pii]
AID - 10.1080/21655979.2021.1980176 [doi]
PST - ppublish
SO  - Bioengineered. 2022 Feb;13(2):4468-4480. doi: 10.1080/21655979.2021.1980176.