PMID- 35167621
OWN - NLM
STAT- MEDLINE
DCOM- 20220224
LR  - 20220224
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 17
IP  - 2
DP  - 2022
TI  - Cryptic MYC insertions in Burkitt lymphoma: New data and a review of the 
      literature.
PG  - e0263980
LID - 10.1371/journal.pone.0263980 [doi]
LID - e0263980
AB  - The occurrence of MYC-negative Burkitt lymphoma (BL) has been discussed for many 
      years. The real frequency of the MYC insertion in MYC-negative BL is still 
      unknown. Fine-needle aspiration biopsies of 108 consecutive patients with 
      clinicopathologically suspected BL (suspBL) were evaluated by flow cytometry, 
      classical cytogenetics, and fluorescence in situ hybridization (FISH). We found 
      12 cases (11%) without the MYC rearrangement by FISH with a MYC breakapart probe: 
      two patients (1.9%) with cryptic MYC/IGH fusion (finally diagnosed as BL) and 10 
      patients (9.3%) with 11q gain/loss (finally diagnosed as Burkitt-like lymphoma 
      with 11q aberration). The exact breakpoints of the cryptic MYC/IGH were 
      investigated by next-generation sequencing. The MYC insertions' breakpoints were 
      identified in PVT1 in the first case, and 42 kb upstream of 5'MYC in the second 
      case. To date, a molecular characterization of the MYC insertion in BL has only 
      been reported in one case. Detailed descriptions of our MYC insertions in a 
      routinely and consecutively diagnosed suspBL cohort will contribute to resolving 
      the issue of MYC negativity in BL. In our opinion, the presence of the MYC 
      insertions in BL and other lymphomas might be underestimated, because routine 
      genetic diagnostics are usually based on FISH only, without karyotyping.
FAU - Woroniecka, Renata
AU  - Woroniecka R
AUID- ORCID: 0000-0003-2531-2775
AD  - Cytogenetic Laboratory, Maria Sklodowska-Curie National Research Institute of 
      Oncology, Warsaw, Poland.
FAU - Rymkiewicz, Grzegorz
AU  - Rymkiewicz G
AD  - Department of Pathology and Laboratory Diagnostics, Flow Cytometry Laboratory, 
      Maria Sklodowska - Curie National Research Institute of Oncology, Warsaw, Poland.
FAU - Szafron, Lukasz M
AU  - Szafron LM
AUID- ORCID: 0000-0003-1670-0274
AD  - Department of Cancer Biology, Maria Sklodowska-Curie National Research Institute 
      of Oncology, Warsaw, Poland.
FAU - Blachnio, Katarzyna
AU  - Blachnio K
AD  - Department of Pathology and Laboratory Diagnostics, Flow Cytometry Laboratory, 
      Maria Sklodowska - Curie National Research Institute of Oncology, Warsaw, Poland.
FAU - Szafron, Laura A
AU  - Szafron LA
AD  - Department of Cancer Biology, Maria Sklodowska-Curie National Research Institute 
      of Oncology, Warsaw, Poland.
FAU - Bystydzienski, Zbigniew
AU  - Bystydzienski Z
AD  - Department of Pathology and Laboratory Diagnostics, Flow Cytometry Laboratory, 
      Maria Sklodowska - Curie National Research Institute of Oncology, Warsaw, Poland.
FAU - Pienkowska-Grela, Barbara
AU  - Pienkowska-Grela B
AD  - Cytogenetic Laboratory, Maria Sklodowska-Curie National Research Institute of 
      Oncology, Warsaw, Poland.
FAU - Borkowska, Klaudia
AU  - Borkowska K
AD  - Cytogenetic Laboratory, Maria Sklodowska-Curie National Research Institute of 
      Oncology, Warsaw, Poland.
FAU - Rygier, Jolanta
AU  - Rygier J
AD  - Cytogenetic Laboratory, Maria Sklodowska-Curie National Research Institute of 
      Oncology, Warsaw, Poland.
FAU - Kotyl, Aleksandra
AU  - Kotyl A
AD  - Cytogenetic Laboratory, Maria Sklodowska-Curie National Research Institute of 
      Oncology, Warsaw, Poland.
FAU - Malawska, Natalia
AU  - Malawska N
AD  - Cytogenetic Laboratory, Maria Sklodowska-Curie National Research Institute of 
      Oncology, Warsaw, Poland.
FAU - Wojtkowska, Katarzyna
AU  - Wojtkowska K
AD  - Cytogenetic Laboratory, Maria Sklodowska-Curie National Research Institute of 
      Oncology, Warsaw, Poland.
FAU - Parada, Joanna
AU  - Parada J
AD  - Department of Cancer Biology, Maria Sklodowska-Curie National Research Institute 
      of Oncology, Warsaw, Poland.
FAU - Borysiuk, Anita
AU  - Borysiuk A
AD  - Department of Pathology and Laboratory Diagnostics, Flow Cytometry Laboratory, 
      Maria Sklodowska - Curie National Research Institute of Oncology, Warsaw, Poland.
FAU - Murcia Pienkowski, Victor
AU  - Murcia Pienkowski V
AD  - Department of Medical Genetics, Medical University of Warsaw, Warsaw, Poland.
FAU - Rydzanicz, Malgorzata
AU  - Rydzanicz M
AD  - Department of Medical Genetics, Medical University of Warsaw, Warsaw, Poland.
FAU - Grygalewicz, Beata
AU  - Grygalewicz B
AD  - Cytogenetic Laboratory, Maria Sklodowska-Curie National Research Institute of 
      Oncology, Warsaw, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20220215
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (MYC protein, human)
RN  - 0 (Proto-Oncogene Proteins c-myc)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biopsy, Fine-Needle
MH  - Burkitt Lymphoma/genetics/*pathology
MH  - Child
MH  - Child, Preschool
MH  - Chromosome Breakpoints
MH  - Female
MH  - High-Throughput Nucleotide Sequencing
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping/*methods
MH  - Male
MH  - Middle Aged
MH  - *Mutagenesis, Insertional
MH  - Proto-Oncogene Proteins c-myc/*genetics
MH  - Sequence Analysis, DNA/*methods
MH  - Young Adult
PMC - PMC8846522
COIS- The authors have declared that no competing interests exist.
EDAT- 2022/02/16 06:00
MHDA- 2022/02/25 06:00
PMCR- 2022/02/15
CRDT- 2022/02/15 17:14
PHST- 2021/09/03 00:00 [received]
PHST- 2022/02/01 00:00 [accepted]
PHST- 2022/02/15 17:14 [entrez]
PHST- 2022/02/16 06:00 [pubmed]
PHST- 2022/02/25 06:00 [medline]
PHST- 2022/02/15 00:00 [pmc-release]
AID - PONE-D-21-27800 [pii]
AID - 10.1371/journal.pone.0263980 [doi]
PST - epublish
SO  - PLoS One. 2022 Feb 15;17(2):e0263980. doi: 10.1371/journal.pone.0263980. 
      eCollection 2022.