PMID- 35168110
OWN - NLM
STAT- MEDLINE
DCOM- 20220324
LR  - 20220531
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 145
DP  - 2022 Jul
TI  - Effects of IFNgamma and IL4 rich microenvironment on porcine monocyte-derived 
      dendritic cell activation in vitro.
PG  - 54-62
LID - S0034-5288(22)00047-9 [pii]
LID - 10.1016/j.rvsc.2022.02.010 [doi]
AB  - Dendritic cells (DCs) represent a heterogeneous group of major antigen-presenting 
      cells, responding to different stimuli in their microenvironment. They are able 
      to activate naive T-cells and drive their polarization towards effector types. 
      However, the effect of different Th-polarizing cytokine microenvironment on 
      porcine DCs remains poorly described. Therefore, the effect of IFNgamma or IL4 rich 
      microenvironment on porcine monocyte-derived dendritic cells and their 
      activation, antigen recognition and cytokine secretion towards T-cell 
      polarization were studied in vitro. IFNgamma-rich microenvironment induced a higher 
      proinflammatory response and release of Th1/Th17-polarizing cytokines (IL1beta, 
      IL12p35, IL23p19), while anti-inflammatory properties (IL10, TGFbeta) was not 
      affected by a cytokine microenvironment. From the achieved results, we propose 
      that different cytokine microenvironment has the potential to modulate dendritic 
      cells and their ability to activate T-cells.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Oreskovic, Zrinka
AU  - Oreskovic Z
AD  - Department of Infectious Diseases and Preventive Medicine, Veterinary Research 
      Institute, Hudcova 296/70, 62100 Brno, Czech Republic. Electronic address: 
      oreskovic@vri.cz.
FAU - Leva, Lenka
AU  - Leva L
AD  - Department of Infectious Diseases and Preventive Medicine, Veterinary Research 
      Institute, Hudcova 296/70, 62100 Brno, Czech Republic. Electronic address: 
      leva@vri.cz.
FAU - Chlebova, Katarina
AU  - Chlebova K
AD  - Department of Infectious Diseases and Preventive Medicine, Veterinary Research 
      Institute, Hudcova 296/70, 62100 Brno, Czech Republic. Electronic address: 
      chlebova@vri.cz.
FAU - Hlavova, Karolina
AU  - Hlavova K
AD  - Department of Infectious Diseases and Preventive Medicine, Veterinary Research 
      Institute, Hudcova 296/70, 62100 Brno, Czech Republic. Electronic address: 
      hlavova@vri.cz.
FAU - Tesarik, Radek
AU  - Tesarik R
AD  - Department of Infectious Diseases and Preventive Medicine, Veterinary Research 
      Institute, Hudcova 296/70, 62100 Brno, Czech Republic. Electronic address: 
      tesarik@vri.cz.
FAU - Gebauer, Jan
AU  - Gebauer J
AD  - Department of Infectious Diseases and Preventive Medicine, Veterinary Research 
      Institute, Hudcova 296/70, 62100 Brno, Czech Republic. Electronic address: 
      gebauer@vri.cz.
FAU - Faldyna, Martin
AU  - Faldyna M
AD  - Department of Infectious Diseases and Preventive Medicine, Veterinary Research 
      Institute, Hudcova 296/70, 62100 Brno, Czech Republic. Electronic address: 
      faldyna@vri.cz.
LA  - eng
PT  - Journal Article
DEP - 20220206
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Cytokines)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cytokines/metabolism
MH  - Dendritic Cells
MH  - Interferon-gamma/*metabolism
MH  - Interleukin-4
MH  - Lymphocyte Activation
MH  - *Monocytes
MH  - Swine
MH  - *Th1 Cells/metabolism
OTO - NOTNLM
OT  - Cytokines
OT  - Dendritic cell
OT  - Innate immunity
OT  - Lymphocyte polarization
EDAT- 2022/02/16 06:00
MHDA- 2022/03/25 06:00
CRDT- 2022/02/15 20:15
PHST- 2021/12/04 00:00 [received]
PHST- 2022/01/31 00:00 [revised]
PHST- 2022/02/02 00:00 [accepted]
PHST- 2022/02/16 06:00 [pubmed]
PHST- 2022/03/25 06:00 [medline]
PHST- 2022/02/15 20:15 [entrez]
AID - S0034-5288(22)00047-9 [pii]
AID - 10.1016/j.rvsc.2022.02.010 [doi]
PST - ppublish
SO  - Res Vet Sci. 2022 Jul;145:54-62. doi: 10.1016/j.rvsc.2022.02.010. Epub 2022 Feb 
      6.