PMID- 35169905
OWN - NLM
STAT- MEDLINE
DCOM- 20220421
LR  - 20230502
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Print)
IS  - 1618-2642 (Linking)
VI  - 414
IP  - 11
DP  - 2022 May
TI  - Miniature mass spectrometer-based point-of-care assay for cabotegravir and 
      rilpivirine in whole blood.
PG  - 3387-3395
LID - 10.1007/s00216-022-03954-3 [doi]
AB  - HIV prevention and treatment with injectable cabotegravir and/or rilpivirine 
      administered once every 4 to 8 weeks is an attractive alternative to daily 
      therapy. Prescribed dosage and drug concentrations in plasma are based on patient 
      data collected in clinical trials, but actual patients are expected to exhibit 
      more variability in drug concentrations, which is important to quantify. Here, we 
      demonstrate the first quantitative point-of-care assay with a miniature mass 
      spectrometer to assess these drug concentrations in whole blood. Quantitative 
      performance is obtained using paper spray ionization in combination with tandem 
      mass spectrometry (MS/MS) in the clinically relevant concentration range of both 
      drugs. Limits of quantitation (LoQs) of cabotegravir and rilpivirine are measured 
      to be 750 ng/mL and 20 ng/mL, respectively. The assay turnaround time is < 4 min, 
      and strong linear relationships are established between MS/MS responses and 
      concentration, with percentage of relative standard deviations (RSDs) that 
      are <15% at concentrations above the LoQs. The speed, portability, low power 
      consumption, and specificity offered by the miniature instrument should make it 
      an appropriate platform for measuring drug concentrations in a walk-in clinic 
      using small volumes of patient blood.
CI  - (c) 2022. Springer-Verlag GmbH Germany, part of Springer Nature.
FAU - Pandey, Sangeeta
AU  - Pandey S
AD  - Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA.
FAU - Hu, Yanyang
AU  - Hu Y
AD  - Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA.
FAU - Bushman, Lane R
AU  - Bushman LR
AD  - Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado 
      Denver, Aurora, CO, 80045, USA.
FAU - Castillo-Mancilla, Jose
AU  - Castillo-Mancilla J
AD  - Division of Infectious Diseases, School of Medicine, University of 
      Colorado-Anschutz Medical Campus, Aurora, CO, 80045, USA.
FAU - Anderson, Peter L
AU  - Anderson PL
AD  - Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado 
      Denver, Aurora, CO, 80045, USA. peter.anderson@cuanschutz.edu.
FAU - Cooks, R Graham
AU  - Cooks RG
AD  - Department of Chemistry, Purdue University, West Lafayette, IN, 47907, USA. 
      cooks@purdue.edu.
LA  - eng
GR  - R01 AI122298/AI/NIAID NIH HHS/United States
GR  - FLIR Basic Research Award #18057244/Teledyne FLIR/
GR  - R01AI122298/GF/NIH HHS/United States
PT  - Journal Article
DEP - 20220215
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Anti-HIV Agents)
RN  - 0 (Diketopiperazines)
RN  - 0 (Pyridones)
RN  - FI96A8X663 (Rilpivirine)
RN  - HMH0132Z1Q (cabotegravir)
SB  - IM
MH  - *Anti-HIV Agents
MH  - Diketopiperazines
MH  - *HIV Infections/drug therapy
MH  - *HIV-1
MH  - Humans
MH  - Point-of-Care Systems
MH  - Pyridones
MH  - Rilpivirine/adverse effects
MH  - Tandem Mass Spectrometry
PMC - PMC9018536
MID - NIHMS1783438
OTO - NOTNLM
OT  - Ambient ionization
OT  - Antiretrovirals
OT  - Drug quantitation
OT  - HIV treatment
OT  - Paper spray
COIS- Conflict of interest statement The authors declare that they have no conflicts of 
      interest.
EDAT- 2022/02/17 06:00
MHDA- 2022/04/22 06:00
PMCR- 2023/05/01
CRDT- 2022/02/16 05:51
PHST- 2021/11/25 00:00 [received]
PHST- 2022/02/01 00:00 [accepted]
PHST- 2022/01/07 00:00 [revised]
PHST- 2022/02/17 06:00 [pubmed]
PHST- 2022/04/22 06:00 [medline]
PHST- 2022/02/16 05:51 [entrez]
PHST- 2023/05/01 00:00 [pmc-release]
AID - 10.1007/s00216-022-03954-3 [pii]
AID - 10.1007/s00216-022-03954-3 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2022 May;414(11):3387-3395. doi: 10.1007/s00216-022-03954-3. 
      Epub 2022 Feb 15.