PMID- 35170676
OWN - NLM
STAT- MEDLINE
DCOM- 20220217
LR  - 20220225
IS  - 1678-8060 (Electronic)
IS  - 0074-0276 (Print)
IS  - 0074-0276 (Linking)
VI  - 116
DP  - 2022
TI  - Evaluation of LAMP for the diagnosis of Loa loa infection in dried blood spots 
      compared to PCR-based assays and microscopy.
PG  - e210210
LID - S0074-02762021000101111 [pii]
LID - 10.1590/0074-02760210210 [doi]
LID - e210210
AB  - BACKGROUND: Loa loa is a filarial species found exclusively in West and Central 
      Africa. Microscopy is the traditional diagnosis method for human loiasis. Several 
      molecular methods have developed as an alternative approach for identification of 
      L. loa filarial parasites. OBJECTIVES: The aim of this study was to evaluate a 
      Loa-Loop-mediated isothermal amplification (LAMP) assay to diagnose loiasis 
      disease on dried blood spots (DBS) samples, compared to microscopy, filaria-real 
      time-polymerase chain reaction (PCR) and nested-Loa PCR. METHODS: A total of 100 
      DBS samples and 100 blood smears were used for this study. DNA was extracted 
      using saponin/Chelex method. DNA isolated was assayed by a Loa-LAMP assay in 
      parallel to microscopy, filaria-real time PCR and nested-Loa PCR. The 
      sensitivities and specificities of Loa-LAMP assay was computed comparing to each 
      one of the reference methods. FINDINGS: Loa-LAMP's sensitivity was more than 90% 
      and specificity was nearly 100% when compared to molecular methods. On the other 
      hand, sensitivity was decreased a bit when Loa-LAMP faced microscopy, but keeping 
      the other statistical values high. MAIN CONCLUSIONS: Loa-LAMP is an appropriate 
      method for loiasis diagnosis in endemic areas. Though, it has disadvantages like 
      the reagents' high price at the moment and not to be able to detect more filarial 
      species at once.
FAU - Ta-Tang, Thuy-Huong
AU  - Ta-Tang TH
AUID- ORCID: 0000-0001-7058-4839
AD  - National Centre of Tropical Medicine, Institute of Health Carlos III, Malaria and 
      Neglected Tropical Diseases Laboratory, Madrid, Spain.
AD  - Cooperative Research Network on Tropical Diseases (Red de Investigacion 
      Colaborativa en Enfermedades Tropicales), Madrid, Spain.
FAU - Berzosa, Pedro
AU  - Berzosa P
AD  - National Centre of Tropical Medicine, Institute of Health Carlos III, Malaria and 
      Neglected Tropical Diseases Laboratory, Madrid, Spain.
AD  - Cooperative Research Network on Tropical Diseases (Red de Investigacion 
      Colaborativa en Enfermedades Tropicales), Madrid, Spain.
FAU - Rubio, Jose Miguel
AU  - Rubio JM
AD  - National Microbiology Centre, Institute of Health Carlos III, Malaria and 
      Emerging Parasitic Diseases Laboratory, Madrid, Spain.
AD  - Cooperative Research Network on Tropical Diseases (Red de Investigacion 
      Colaborativa en Enfermedades Tropicales), Madrid, Spain.
FAU - Romay-Barja, Maria
AU  - Romay-Barja M
AD  - National Centre of Tropical Medicine, Institute of Health Carlos III, Malaria and 
      Neglected Tropical Diseases Laboratory, Madrid, Spain.
AD  - Cooperative Research Network on Tropical Diseases (Red de Investigacion 
      Colaborativa en Enfermedades Tropicales), Madrid, Spain.
FAU - Ncogo, Policarpo
AU  - Ncogo P
AD  - Ministry of Health, Malabo, Equatorial Guinea.
AD  - Fundacion Estatal, Salud, Infancia y Bienestar Social, Madrid, Spain.
FAU - Agudo, Diego
AU  - Agudo D
AD  - Universidad Complutense de Madrid, Facultad de Ciencias Biologicas, Madrid, 
      Spain.
FAU - Herrador, Zaida
AU  - Herrador Z
AD  - National Centre of Tropical Medicine, Institute of Health Carlos III, Malaria and 
      Neglected Tropical Diseases Laboratory, Madrid, Spain.
AD  - Cooperative Research Network on Tropical Diseases (Red de Investigacion 
      Colaborativa en Enfermedades Tropicales), Madrid, Spain.
FAU - Cerrada-Galvez, Laura
AU  - Cerrada-Galvez L
AD  - Universidad Complutense de Madrid, Facultad de Ciencias Biologicas, Madrid, 
      Spain.
FAU - Benito, Agustin
AU  - Benito A
AD  - National Centre of Tropical Medicine, Institute of Health Carlos III, Malaria and 
      Neglected Tropical Diseases Laboratory, Madrid, Spain.
AD  - Cooperative Research Network on Tropical Diseases (Red de Investigacion 
      Colaborativa en Enfermedades Tropicales), Madrid, Spain.
LA  - eng
PT  - Journal Article
DEP - 20220211
PL  - Brazil
TA  - Mem Inst Oswaldo Cruz
JT  - Memorias do Instituto Oswaldo Cruz
JID - 7502619
RN  - LAMP assay
SB  - IM
MH  - Humans
MH  - *Loiasis/diagnosis
MH  - Microscopy
MH  - Molecular Diagnostic Techniques
MH  - Nucleic Acid Amplification Techniques
MH  - Real-Time Polymerase Chain Reaction
MH  - Sensitivity and Specificity
PMC - PMC8843042
EDAT- 2022/02/17 06:00
MHDA- 2022/02/19 06:00
PMCR- 2022/02/11
CRDT- 2022/02/16 08:43
PHST- 2021/06/16 00:00 [received]
PHST- 2021/12/14 00:00 [accepted]
PHST- 2022/02/16 08:43 [entrez]
PHST- 2022/02/17 06:00 [pubmed]
PHST- 2022/02/19 06:00 [medline]
PHST- 2022/02/11 00:00 [pmc-release]
AID - S0074-02762021000101111 [pii]
AID - 10.1590/0074-02760210210 [doi]
PST - epublish
SO  - Mem Inst Oswaldo Cruz. 2022 Feb 11;116:e210210. doi: 10.1590/0074-02760210210. 
      eCollection 2022.