PMID- 35174108
OWN - NLM
STAT- MEDLINE
DCOM- 20220406
LR  - 20220406
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 12
DP  - 2022
TI  - Commensal Bacteria in the Cystic Fibrosis Airway Microbiome Reduce P. aeruginosa 
      Induced Inflammation.
PG  - 824101
LID - 10.3389/fcimb.2022.824101 [doi]
LID - 824101
AB  - Chronic Pseudomonas aeruginosa infections play an important role in the progress 
      of lung disease in patients suffering from cystic fibrosis (CF). Recent studies 
      indicate that polymicrobial microbiome profiles in the airway are associated with 
      less inflammation. Thus, the hypothesis was raised that certain commensal 
      bacteria might protect the host from inflammation. We therefore performed a 
      screening study with commensals isolated from CF airway microbiome samples to 
      identify potential beneficial commensals. We isolated more than 80 aerobic or 
      facultative anaerobic commensal strains, including strains from genera 
      Streptococcus, Neisseria, Actinomyces, Corynebacterium, Dermabacter, Micrococcus 
      and Rothia. Through a screening experiment of co-infection in human epithelial 
      cell lines, we identified multiple commensal strains, especially strains 
      belonging to Streptococcus mitis, that reduced P. aeruginosa triggered 
      inflammatory responses. The results were confirmed by co-infection experiments in 
      ex-vivo precision cut lung slices (PCLS) from mice. The underlying mechanisms of 
      the complex host-pathogen-commensal crosstalk were investigated from both the 
      host and the bacterial sides with a focus on S. mitis. Transcriptome changes in 
      the host in response to co-infection and mono-infection were evaluated, and the 
      results indicated that several signalling pathways mediating inflammatory 
      responses were downregulated by co-infection with S. mitis and P. aeruginosa 
      compared to P. aeruginosa mono-infection, such as neutrophil extracellular trap 
      formation. The genomic differences among S. mitis strains with and without 
      protective effects were investigated by whole genome sequencing, revealing genes 
      only present in the S. mitis strains showing protective effects. In summary, 
      through both in vitro and ex vivo studies, we could identify a variety of 
      commensal strains that may reduce host inflammatory responses induced by P. 
      aeruginosa infection. These findings support the hypothesis that CF airway 
      commensals may protect the host from inflammation.
CI  - Copyright (c) 2022 Tony-Odigie, Wilke, Boutin, Dalpke and Yi.
FAU - Tony-Odigie, Andrew
AU  - Tony-Odigie A
AD  - Institute of Medical Microbiology and Virology, University Hospital Carl Gustav 
      Carus, Technische Universitat Dresden, Dresden, Germany.
FAU - Wilke, Leonie
AU  - Wilke L
AD  - Institute of Medical Microbiology and Virology, University Hospital Carl Gustav 
      Carus, Technische Universitat Dresden, Dresden, Germany.
FAU - Boutin, Sebastien
AU  - Boutin S
AD  - Translational Lung Research Center (TLRC), Member of the German Center for Lung 
      Research (DZL), Heidelberg, Germany.
AD  - Department of Infectious Diseases, Medical Microbiology and Hygiene, University 
      of Heidelberg, Heidelberg, Germany.
FAU - Dalpke, Alexander H
AU  - Dalpke AH
AD  - Institute of Medical Microbiology and Virology, University Hospital Carl Gustav 
      Carus, Technische Universitat Dresden, Dresden, Germany.
FAU - Yi, Buqing
AU  - Yi B
AD  - Institute of Medical Microbiology and Virology, University Hospital Carl Gustav 
      Carus, Technische Universitat Dresden, Dresden, Germany.
LA  - eng
SI  - figshare/10.6084/m9.figshare.17118539
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220131
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
SB  - IM
MH  - Animals
MH  - *Cystic Fibrosis/microbiology
MH  - Humans
MH  - Inflammation/microbiology
MH  - Lung/microbiology
MH  - Mice
MH  - *Microbiota
MH  - *Pseudomonas Infections/complications
MH  - Pseudomonas aeruginosa/genetics
PMC - PMC8842722
OTO - NOTNLM
OT  - Pseudomonas aeruginosa
OT  - airway microbiome
OT  - beneficial commensal
OT  - cystic fibrosis
OT  - inflammation inhibition
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/02/18 06:00
MHDA- 2022/04/07 06:00
PMCR- 2022/01/01
CRDT- 2022/02/17 05:37
PHST- 2021/11/28 00:00 [received]
PHST- 2022/01/12 00:00 [accepted]
PHST- 2022/02/17 05:37 [entrez]
PHST- 2022/02/18 06:00 [pubmed]
PHST- 2022/04/07 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2022.824101 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2022 Jan 31;12:824101. doi: 
      10.3389/fcimb.2022.824101. eCollection 2022.