PMID- 35174243
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220219
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 8
DP  - 2021
TI  - A Virulent Trueperella pyogenes Isolate, Which Causes Severe Bronchoconstriction 
      in Porcine Precision-Cut Lung Slices.
PG  - 824349
LID - 10.3389/fvets.2021.824349 [doi]
LID - 824349
AB  - Trueperella pyogenes causes disease in cattle, sheep, goats and swine, and is 
      involved occasionally in human disease worldwide. Most reports implicating T. 
      pyogenes have been associated with clinical cases, whereas no report has focused 
      on pathogenicity of T. pyogenes in mouse models or precision-cut lung slice 
      (PCLS) cultures from swine. Here, we isolated and identified a virulent, 
      beta-hemolytic, multidrug-resistant T. pyogenes strain named 20121, which harbors 
      the virulence marker genes fimA, fimE, nanH, nanP and plo. It was found to be 
      highly resistant to erythromycin, azithromycin and medemycin. Strain 20121 was 
      pathogenic in mouse infection models, displaying pulmonary congestion and 
      inflammatory cell infiltration, partial degeneration in epithelial cells of the 
      tracheal and bronchiolar mucosa, a small amount of inflammatory cell infiltration 
      in the submucosa, and bacteria (>10(4) CFU/g) in the lung. Importantly, we used 
      T. pyogenes 20121 to infect porcine precision-cut lung slices (PCLS) cultures for 
      the first time, where it caused severe bronchoconstriction. Furthermore, 
      dexamethasone showed its ability to relieve bronchoconstriction in PCLS caused by 
      T. pyogenes 20121, highlighting dexamethasone may assist antibiotic treatment for 
      clinical T. pyogenes infection. This is the first report of T. pyogenes used to 
      infect and cause bronchoconstriction in porcine PCLS. Our results suggest that 
      porcine PCLS cultures as a valuable 3D organ model for the study of T. pyogenes 
      infection and treatment in vitro.
CI  - Copyright (c) 2022 Qin, Meng, He, Yang, Wang, Tang, Sun, Zhang, Cai and Wang.
FAU - Qin, Lei
AU  - Qin L
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Meng, Fandan
AU  - Meng F
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - He, Haijuan
AU  - He H
AD  - Institute of Animal Husbandry, Heilongjiang Academy of Agriculture Sciences, 
      Harbin, China.
FAU - Yang, Yong-Bo
AU  - Yang YB
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Wang, Gang
AU  - Wang G
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Tang, Yan-Dong
AU  - Tang YD
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Sun, Mingxia
AU  - Sun M
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Zhang, Wenlong
AU  - Zhang W
AD  - College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
FAU - Cai, Xuehui
AU  - Cai X
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
FAU - Wang, Shujie
AU  - Wang S
AD  - National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
LA  - eng
PT  - Journal Article
DEP - 20220131
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC8841747
OTO - NOTNLM
OT  - Trueperella pyogenes
OT  - bronchoconstriction
OT  - infection
OT  - porcine precision-cut lung slices
OT  - virulent
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/02/18 06:00
MHDA- 2022/02/18 06:01
PMCR- 2021/01/01
CRDT- 2022/02/17 05:38
PHST- 2021/11/29 00:00 [received]
PHST- 2021/12/23 00:00 [accepted]
PHST- 2022/02/17 05:38 [entrez]
PHST- 2022/02/18 06:00 [pubmed]
PHST- 2022/02/18 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2021.824349 [doi]
PST - epublish
SO  - Front Vet Sci. 2022 Jan 31;8:824349. doi: 10.3389/fvets.2021.824349. eCollection 
      2021.