PMID- 35175493
OWN - NLM
STAT- MEDLINE
DCOM- 20220404
LR  - 20220405
IS  - 1543-706X (Electronic)
IS  - 1071-2690 (Linking)
VI  - 58
IP  - 2
DP  - 2022 Feb
TI  - Osteogenic differentiation of human induced pluripotent stem cell in the presence 
      of testosterone and 17 beta-estradiol in vitro.
PG  - 179-188
LID - 10.1007/s11626-022-00652-3 [doi]
AB  - Recently, numerous scientific approaches have been explored to treat various 
      diseases using stem cells. In 2006, induced pluripotent stem cell (iPSC) were 
      introduced by Takahashi and Yamanaka and showed the potential of self-renewing 
      and differentiation into all types of targeted cells in vitro. In this 
      investigation, we studied the effect of testosterone (T) individually or in the 
      presence of 17 beta-estradiol (E2) on osteogenic differentiation of human iPSC 
      (hiPSC) during 2 wk. The optimal concentrations of sex steroid hormones were 
      examined by MTT assay and acridine orange (AO) staining. The impact of E2 and T 
      either individually or together as a combination was examined by ALP activity; 
      the content of total mineral calcium, by von Kossa and alizarin red staining. 
      Additionally, the expression rate of osteogenic specific markers was studied via 
      real-time RT-PCR and immunocytochemistry analyses at day 14 of differentiation. 
      The obtained results illustrated that the differentiation medium supplemented 
      with T-E2 increased not only the ALP enzyme activity and the content of calcium 
      but also the osteogenic-related gene and protein expressions on the 14(th) day. 
      Furthermore, the results were confirmed by mineralized matrix staining. In 
      conclusion, these data suggest that T could be used as an effective factor for 
      osteogenic induction of hiPSCs combined with the E2 in bone regeneration.
CI  - (c) 2022. The Society for In Vitro Biology.
FAU - Yarmohammadi, Reyhaneh
AU  - Yarmohammadi R
AD  - Stem Cell and Regenerative Medicine Center of Excellence, Tehran University of 
      Medical Sciences, Tehran, Iran.
FAU - Ghollasi, Marzieh
AU  - Ghollasi M
AD  - Department of Cell and Molecular Biology, Faculty of Biological Sciences, 
      Kharazmi University, P. O. Box, 15719-14911, Tehran, Iran. ghollasi@khu.ac.ir.
FAU - Kheirollahzadeh, Fatemeh
AU  - Kheirollahzadeh F
AD  - Department of Biology, Science and Research Branch, Islamic Azad University, 
      Tehran, Iran.
FAU - Soltanyzadeh, Maryam
AU  - Soltanyzadeh M
AD  - Department of Cell and Molecular Biology, Faculty of Biological Sciences, 
      Kharazmi University, P. O. Box, 15719-14911, Tehran, Iran.
FAU - Heshmati, Masoumeh
AU  - Heshmati M
AD  - Department of Cellular and Molecular Biology, Faculty of Advanced Science and 
      Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
FAU - Amirkhani, Mohammad Amir
AU  - Amirkhani MA
AD  - Stem Cell and Regenerative Medicine Center of Excellence, Tehran University of 
      Medical Sciences, Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20220217
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
RN  - 3XMK78S47O (Testosterone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Estradiol/pharmacology
MH  - Humans
MH  - *Induced Pluripotent Stem Cells/metabolism
MH  - *Mesenchymal Stem Cells
MH  - Osteogenesis
MH  - Testosterone/pharmacology
OTO - NOTNLM
OT  - Estrogen
OT  - Human induced pluripotent stem cells
OT  - Osteogenic differentiation
OT  - Testosterone
EDAT- 2022/02/18 06:00
MHDA- 2022/04/05 06:00
CRDT- 2022/02/17 12:27
PHST- 2021/11/08 00:00 [received]
PHST- 2022/01/23 00:00 [accepted]
PHST- 2022/02/18 06:00 [pubmed]
PHST- 2022/04/05 06:00 [medline]
PHST- 2022/02/17 12:27 [entrez]
AID - 10.1007/s11626-022-00652-3 [pii]
AID - 10.1007/s11626-022-00652-3 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 2022 Feb;58(2):179-188. doi: 
      10.1007/s11626-022-00652-3. Epub 2022 Feb 17.