PMID- 35176539
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231020
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1193
DP  - 2022 Mar 15
TI  - High-throughput analysis of total homocysteine and methylmalonic acid with the 
      efficiency to separate succinic acid in serum and urine via liquid chromatography 
      tandem mass spectrometry.
PG  - 123135
LID - S1570-0232(22)00039-3 [pii]
LID - 10.1016/j.jchromb.2022.123135 [doi]
AB  - Vitamin B12 (VB12) deficiency may lead to hyperhomocysteinemia and methylmalonic 
      acidemia development which are risk factors of cardiovascular disease and nervous 
      system impairment, respectively. However, few analytical methods are available to 
      simultaneously quantify total homocysteine (tHcy) and methylmalonic acid (MMA) 
      due to complex analytical requirements, such as sensitivity at nanomolar 
      concentration, separation performance for succinic acid (SA), an endogenous 
      isomer of MMA, and retention properties for polar compounds. Therefore, we 
      developed and validated a simple and accurate liquid chromatography-tandem mass 
      spectrometry (LC-MS/MS) method for the quantification of tHcy and MMA with the 
      efficient separation of SA in human serum and urine. The clinical performance of 
      the assay was validated according to CLSI C62-A guidelines. The recovery for 
      serum tHcy was 95.2-105.8%, urine tHcy was 98.1-111.5%, serum MMA was 94.6-99.4%, 
      and urine MMA was 101.6-105.6%. In addition, the LC-MS/MS method was found to be 
      reliable based on the value of inter-assay imprecision and total imprecision 
      coefficient variation (CV), matrix effect, and carryover. Standards and samples 
      were stable in -20  degrees C for at least 2 months. The limits of quantifications (LOQs) 
      were 0.074 nmol/mL for tHcy and 0.040 nmol/mL for MMA, which are suitable for 
      detecting tHcy and MMA concentrations in human serum and urine. The concentration 
      of tHcy and MMA in samples collected from 148 subjects were measured using this 
      method. The results suggested that the concentrations of serum tHcy and MMA 
      considerably differed between VB12 sufficient and deficient groups. Serum tHcy 
      and serum MMA concentrations were inversely correlated with VB12 status. Our 
      method represents a rapid technique for estimating tHcy and MMA concentrations in 
      serum and urine samples without the need for derivatization and may be used to 
      assess VB12 status in clinical applications.
CI  - Copyright (c) 2022. Published by Elsevier B.V.
FAU - Ma, Xiaoli
AU  - Ma X
AD  - Department of Laboratory Medicine, Peking Union Medical College Hospital, Peking 
      Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, 
      China.
FAU - Zou, Yutong
AU  - Zou Y
AD  - Department of Laboratory Medicine, Peking Union Medical College Hospital, Peking 
      Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, 
      China.
FAU - Tang, Yueming
AU  - Tang Y
AD  - Department of Laboratory Medicine, Peking Union Medical College Hospital, Peking 
      Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, 
      China.
FAU - Wang, Danchen
AU  - Wang D
AD  - Department of Laboratory Medicine, Peking Union Medical College Hospital, Peking 
      Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, 
      China.
FAU - Zhou, Weiyan
AU  - Zhou W
AD  - National Center for Clinical Laboratories, Beijing Hospital, National Center for 
      Gerontology, Beijing Engineering Research Center of Laboratory Medicine, Beijing, 
      China.
FAU - Yu, Songlin
AU  - Yu S
AD  - Department of Laboratory Medicine, Peking Union Medical College Hospital, Peking 
      Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, 
      China. Electronic address: yusonglinpku@163.com.
FAU - Qiu, Ling
AU  - Qiu L
AD  - Department of Laboratory Medicine, Peking Union Medical College Hospital, Peking 
      Union Medical College & Chinese Academy of Medical Sciences, Beijing 100730, 
      China; State Key Laboratory of Complex Severe and Rare Diseases, Peking Union 
      Medical College Hospital, Peking Union Medical College & Chinese Academy of 
      Medical Sciences, Beijing 100730, China. Electronic address: lingqiubj@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220211
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
SB  - IM
OTO - NOTNLM
OT  - Liquid chromatography-tandem mass spectrometry
OT  - Methylmalonic acid
OT  - Total homocysteine
OT  - vitamin B12 deficiency
EDAT- 2022/02/18 06:00
MHDA- 2022/02/18 06:01
CRDT- 2022/02/17 20:13
PHST- 2021/09/02 00:00 [received]
PHST- 2022/01/06 00:00 [revised]
PHST- 2022/01/19 00:00 [accepted]
PHST- 2022/02/18 06:01 [medline]
PHST- 2022/02/18 06:00 [pubmed]
PHST- 2022/02/17 20:13 [entrez]
AID - S1570-0232(22)00039-3 [pii]
AID - 10.1016/j.jchromb.2022.123135 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2022 Mar 15;1193:123135. doi: 
      10.1016/j.jchromb.2022.123135. Epub 2022 Feb 11.