PMID- 35180101
OWN - NLM
STAT- MEDLINE
DCOM- 20230124
LR  - 20230201
IS  - 1878-3619 (Electronic)
IS  - 0959-2989 (Linking)
VI  - 34
IP  - 1
DP  - 2023
TI  - Immunological characteristics of human umbilical cord mesenchymal stem cells 
      after hepatogenic differentiation.
PG  - 1-11
LID - 10.3233/BME-211331 [doi]
AB  - BACKGROUND: Acute liver failure is one of the most intractable clinical problems. 
      The use of bioartificial livers may solve donor shortage problems. Human 
      umbilical cord mesenchymal stem cells (hUCMSCs) are an excellent seed cell choice 
      for artificial livers because they change their characteristics to resemble 
      hepatocyte-like cells (HLCs) following artificial liver transplantation. 
      OBJECTIVE: This study aimed to determine whether the immunological 
      characteristics of hUCMSCs are changed after being transformed into 
      hepatocyte-like cells. METHODS: HUCMSCs were isolated by the adherent method. The 
      following hUCMSC surface markers were detected using flow cytometry: CD45, CD90, 
      CD105, CD34, and octamer-binding transcription factor 4 (OCT-4). Functional 
      detection of adipogenic differentiation was performed. The hUCMSCs were cultured 
      in complete medium (control group) or induction medium (induction group), and 
      flow cytometry was used to detect cell surface markers. Peritoneal lavage fluid 
      was collected after intraperitoneal injection of 1 x 106 cells/mouse over 
      40 minutes. The leukocyte count, labeled CD45, CD3, CD4 and CD8 antibodies, and 
      flow detection of T lymphocyte subsets were determined using the peritoneal 
      lavage fluid. RESULTS: Using phenotypic and functional identification, hUCMSCs 
      were successfully isolated using a two-step induction method. The surface markers 
      of the hUCMSCs cells changed after HLC induction. In vivo immune results showed 
      that hUCMSCs and HLsC induced leukocyte production. CONCLUSION: Hepatic induction 
      of hUCMSCs changes their cell surface markers. Both HLCs and hUCMSCs cause 
      leukocytosis in vivo, but the immune response induced by HLCs is slightly 
      stronger.
FAU - Yin, Yanfeng
AU  - Yin Y
AD  - Biomedical Research Center, The First Hospital of Kunming (the Affiliated 
      Calmette Hospital of Kunming Medical University), Kunming, China.
FAU - Hu, Zongqiang
AU  - Hu Z
AD  - Department of Hepatobiliary Surgery, The First Hospital of Kunming (the 
      Affiliated Calmette Hospital of Kunming Medical University), Kunming, China.
FAU - Guan, Zheng
AU  - Guan Z
AD  - Biomedical Research Center, The First Hospital of Kunming (the Affiliated 
      Calmette Hospital of Kunming Medical University), Kunming, China.
FAU - Lv, Sha
AU  - Lv S
AD  - Biomedical Research Center, The First Hospital of Kunming (the Affiliated 
      Calmette Hospital of Kunming Medical University), Kunming, China.
FAU - Wang, Yiyin
AU  - Wang Y
AD  - Biomedical Research Center, The First Hospital of Kunming (the Affiliated 
      Calmette Hospital of Kunming Medical University), Kunming, China.
FAU - Su, Wenjun
AU  - Su W
AD  - Biomedical Research Center, The First Hospital of Kunming (the Affiliated 
      Calmette Hospital of Kunming Medical University), Kunming, China.
FAU - Yan, Chuntao
AU  - Yan C
AD  - Biomedical Research Center, The First Hospital of Kunming (the Affiliated 
      Calmette Hospital of Kunming Medical University), Kunming, China.
FAU - Wang, Yiting
AU  - Wang Y
AD  - Biomedical Research Center, The First Hospital of Kunming (the Affiliated 
      Calmette Hospital of Kunming Medical University), Kunming, China.
FAU - Ma, Li
AU  - Ma L
AD  - Biomedical Research Center, The First Hospital of Kunming (the Affiliated 
      Calmette Hospital of Kunming Medical University), Kunming, China.
FAU - Jiang, Jie
AU  - Jiang J
AD  - Department of Hepatobiliary Surgery, The First Hospital of Kunming (the 
      Affiliated Calmette Hospital of Kunming Medical University), Kunming, China.
FAU - Feng, Shiming
AU  - Feng S
AD  - Department of Hepatobiliary Surgery, The First Hospital of Kunming (the 
      Affiliated Calmette Hospital of Kunming Medical University), Kunming, China.
FAU - Li, Li
AU  - Li L
AD  - Department of Hepatobiliary Surgery, The First Hospital of Kunming (the 
      Affiliated Calmette Hospital of Kunming Medical University), Kunming, China.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Biomed Mater Eng
JT  - Bio-medical materials and engineering
JID - 9104021
SB  - IM
MH  - Humans
MH  - Animals
MH  - Mice
MH  - Cell Differentiation
MH  - Liver
MH  - Hepatocytes
MH  - *Mesenchymal Stem Cells
MH  - Umbilical Cord
MH  - *Mesenchymal Stem Cell Transplantation/methods
OTO - NOTNLM
OT  - differentiation
OT  - hUCMSC
OT  - hepatocyte-like cell
OT  - immunogenicity
OT  - liver
EDAT- 2022/02/19 06:00
MHDA- 2023/01/25 06:00
CRDT- 2022/02/18 17:08
PHST- 2022/02/19 06:00 [pubmed]
PHST- 2023/01/25 06:00 [medline]
PHST- 2022/02/18 17:08 [entrez]
AID - BME211331 [pii]
AID - 10.3233/BME-211331 [doi]
PST - ppublish
SO  - Biomed Mater Eng. 2023;34(1):1-11. doi: 10.3233/BME-211331.