PMID- 35180954
OWN - NLM
STAT- MEDLINE
DCOM- 20220311
LR  - 20220311
IS  - 0306-4565 (Print)
IS  - 0306-4565 (Linking)
VI  - 104
DP  - 2022 Feb
TI  - Energy metabolism response of Litopenaeus vannamei to combined stress of acute 
      cold exposure and waterless duration: Implications for physiological regulation 
      and waterless live transport.
PG  - 103149
LID - S0306-4565(21)00317-X [pii]
LID - 10.1016/j.jtherbio.2021.103149 [doi]
AB  - Maintaining the homeostasis of energy metabolism is crucial for organism's stress 
      tolerance and survival. Acute cold exposure (AC) and waterless duration (WD) 
      represent the two predominate abiotic stressors during waterless live transport 
      of Litopenaeus vannamei. Although previous reports have explored the 
      physiological response of L. vannamei to combined stress AC + WD, the roles of 
      energy metabolism response in regulation of stress tolerance remains unknown. The 
      present study comparatively examined the variations of energy metabolism-related 
      indicators in hemolymph (cortisol, hemocyanin, glucose and lactate), 
      hepatopancreas and muscle tissues (levels of lactate and glycogen, activities of 
      hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), lactate 
      dehydrogenase (LDH), succinate dehydrogenase (SDH) and adenosine triphosphatase 
      (ATPase), and ATP levels). Combined stress significantly disturbed the 
      homeostasis of energy metabolism with the increase in levels of hemocyanin, 
      glucose and lactate, and decrease in glycogen and ATP content (P < 0.05). In 
      addition, the activities of HK, PFK, PK, and SDH initially elevated and then 
      decreased with the prolongation of combined stress from 3h to 9h duration, while 
      the activity of lactate dehydrogenase (LDH) remained gradual elevation and ATPase 
      activity decreased in a duration time dependent manner throughout the experiment. 
      These alterations revealed that exposure to combined stress could accelerate 
      anaerobic metabolism at initial stage and inhibit aerobic metabolism in a 
      duration time-dependent manner, following with the reduction of energy 
      biosynthesis and the disturbance of energy metabolism equilibrium. On the other 
      hand, the progressive impairment on hepatopancreas tissue was observed under 
      combined stress. In summary, the deficiency of ATP supply and histopathological 
      injures on hepatopancreas tissue might the underlying mechanisms inducing 
      mortality of L. vannamei during live transport.
CI  - Copyright (c) 2021. Published by Elsevier Ltd.
FAU - Xu, Defeng
AU  - Xu D
AD  - College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 
      524088, China; Guangdong Provincial Key Laboratory of Aquatic Product Processing 
      and Safety, Zhanjiang, 524088, China; Collaborative Innovation Center of Seafood 
      Deep Processing, Dalian Polytechnic University, Dalian, 116034, China. Electronic 
      address: 13827198525@163.com.
FAU - Wu, Jiaxin
AU  - Wu J
AD  - College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 
      524088, China; Guangdong Provincial Key Laboratory of Aquatic Product Processing 
      and Safety, Zhanjiang, 524088, China.
FAU - Sun, Lijun
AU  - Sun L
AD  - College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 
      524088, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian 
      Polytechnic University, Dalian, 116034, China.
FAU - Qin, Xiaoming
AU  - Qin X
AD  - College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 
      524088, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian 
      Polytechnic University, Dalian, 116034, China.
FAU - Fan, Xiuping
AU  - Fan X
AD  - College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 
      524088, China; Guangdong Provincial Key Laboratory of Aquatic Product Processing 
      and Safety, Zhanjiang, 524088, China.
FAU - Zheng, Xiaoxian
AU  - Zheng X
AD  - College of Food Science and Technology, Guangdong Ocean University, Zhanjiang, 
      524088, China; Guangdong Provincial Key Laboratory of Aquatic Product Processing 
      and Safety, Zhanjiang, 524088, China.
LA  - eng
PT  - Journal Article
DEP - 20211226
PL  - England
TA  - J Therm Biol
JT  - Journal of thermal biology
JID - 7600115
RN  - 9005-79-2 (Glycogen)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Anaerobiosis
MH  - Animals
MH  - *Energy Metabolism
MH  - Glucose/metabolism
MH  - Glycogen/metabolism
MH  - Hemolymph/metabolism
MH  - Hepatopancreas/metabolism
MH  - Homeostasis
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Muscles/metabolism
MH  - Penaeidae/metabolism/*physiology
MH  - Stress, Physiological/*physiology
OTO - NOTNLM
OT  - Combined stress
OT  - Energy metabolism response
OT  - Hepatopancreas impairment
OT  - L. vannamei
OT  - Live transport
EDAT- 2022/02/20 06:00
MHDA- 2022/03/12 06:00
CRDT- 2022/02/19 05:24
PHST- 2021/07/21 00:00 [received]
PHST- 2021/10/28 00:00 [revised]
PHST- 2021/11/29 00:00 [accepted]
PHST- 2022/02/19 05:24 [entrez]
PHST- 2022/02/20 06:00 [pubmed]
PHST- 2022/03/12 06:00 [medline]
AID - S0306-4565(21)00317-X [pii]
AID - 10.1016/j.jtherbio.2021.103149 [doi]
PST - ppublish
SO  - J Therm Biol. 2022 Feb;104:103149. doi: 10.1016/j.jtherbio.2021.103149. Epub 2021 
      Dec 26.