PMID- 35182461 OWN - NLM STAT- MEDLINE DCOM- 20220929 LR - 20220929 IS - 1865-1682 (Electronic) IS - 1865-1674 (Linking) VI - 69 IP - 5 DP - 2022 Sep TI - Novel characteristics of Chinese NADC34-like PRRSV during 2020-2021. PG - e3215-e3224 LID - 10.1111/tbed.14485 [doi] AB - NADC34-like porcine reproductive and respiratory syndrome virus (PRRSV) strains were first detected in China in 2017, with epidemic potential. In this study, the phylogenetic, epidemic, and recombinant properties of NADC34-like PRRSV in China were evaluated comprehensively. From 2020 to October 2021, 82 NADC34-like PRRSV isolates were obtained from 433 PRRSV-positive clinical samples. These strains accounted for 11.5% and 28.6% of positives in 2020 and 2021, respectively, and have spread to eight provinces. We selected 15 samples for whole-genome sequencing, revealing genome lengths of 15,009-15,113 nt. Phylogenetic analysis revealed that Chinese NADC34-like strains cluster with American sublineage 1.5 strains and do not form an independent branch. Recombination analysis revealed that six of fifteen complete genome sequences were derived from recombination between NADC34-like and NADC30-like or HP-PRRSV; all of the strains recombined with local strains in China, exhibiting a complex recombination pattern. Partial Nsp2 sequence alignment showed that nine of fifteen isolates had a 100 aa continuous deletion (similar to that in IA/2014/NADC34); other isolates had a 131 aa discontinuous deletion (similar to that in NADC30). Five of them also had additional amino acid deletions, all of which are reported for the first time here. In the last 2 years, NADC34-like PRRSV has become one of the main epidemic strains in some areas of China; it has changed significantly, its homology has decreased significantly, and it has undergone complex recombination with local Chinese strains. These results are of great significance for understanding the current epidemic situation of PRRSV in China. CI - (c) 2022 Wiley-VCH GmbH. FAU - Xu, Hu AU - Xu H AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Li, Chao AU - Li C AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Li, Wansheng AU - Li W AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Zhao, Jing AU - Zhao J AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Gong, Bangjun AU - Gong B AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Sun, Qi AU - Sun Q AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Tang, Yan-Dong AU - Tang YD AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Xiang, Lirun AU - Xiang L AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Leng, Chaoliang AU - Leng C AUID- ORCID: 0000-0002-9884-5313 AD - Henan Key Laboratory of Insect Biology in Funiu Mountain, Henan Provincial Engineering Laboratory of Insects Bioreactor, China-UK-NYNU-RRes Joint Laboratory of Insect Biology, Nanyang Normal University, Nanyang, China. FAU - Peng, Jinmei AU - Peng J AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Wang, Qian AU - Wang Q AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Meng, Fandan AU - Meng F AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Yu, Ying AU - Yu Y AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China. FAU - An, Tongqing AU - An T AUID- ORCID: 0000-0003-4619-4587 AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Cai, Xuehui AU - Cai X AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Tian, Zhi-Jun AU - Tian ZJ AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. FAU - Zhang, Hongliang AU - Zhang H AUID- ORCID: 0000-0003-0705-6800 AD - State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China. LA - eng GR - 32172890/National Natural Science Foundation of China/ GR - 32002315/National Natural Science Foundation of China/ GR - 2020M680788/China Postdoctoral Fund/ GR - YQ2019C032/Natural Science Foundation of Heilongjiang Province/ GR - YQ2019C030/Natural Science Foundation of Heilongjiang Province/ GR - SKLVBF202115/State Key Laboratory of Veterinary Biotechnology Foundation/ PT - Journal Article DEP - 20220303 PL - Germany TA - Transbound Emerg Dis JT - Transboundary and emerging diseases JID - 101319538 RN - 0 (Amino Acids) SB - IM MH - Amino Acids MH - Animals MH - China/epidemiology MH - Genetic Variation MH - Genome, Viral MH - Phylogeny MH - *Porcine Reproductive and Respiratory Syndrome/epidemiology MH - *Porcine respiratory and reproductive syndrome virus/genetics MH - Recombination, Genetic MH - Swine MH - *Swine Diseases OTO - NOTNLM OT - NADC34-like PRRSV OT - endemic strain OT - novel characterization OT - recombination EDAT- 2022/02/20 06:00 MHDA- 2022/09/30 06:00 CRDT- 2022/02/19 17:03 PHST- 2022/01/13 00:00 [revised] PHST- 2021/12/22 00:00 [received] PHST- 2022/01/24 00:00 [accepted] PHST- 2022/02/20 06:00 [pubmed] PHST- 2022/09/30 06:00 [medline] PHST- 2022/02/19 17:03 [entrez] AID - 10.1111/tbed.14485 [doi] PST - ppublish SO - Transbound Emerg Dis. 2022 Sep;69(5):e3215-e3224. doi: 10.1111/tbed.14485. Epub 2022 Mar 3.