PMID- 35182973
OWN - NLM
STAT- MEDLINE
DCOM- 20220411
LR  - 20220411
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 600
DP  - 2022 Apr 16
TI  - Paeonol protects against acute pancreatitis by inhibiting M1 macrophage 
      polarization via the NLRP3 inflammasomes pathway.
PG  - 35-43
LID - S0006-291X(22)00201-7 [pii]
LID - 10.1016/j.bbrc.2022.02.019 [doi]
AB  - The excessive inflammatory response mediated by macrophage is one of the key 
      factors for the progress of acute pancreatitis (AP). Paeonol (Pae) was 
      demonstrated to exert multiple anti-inflammatory effects. However, the role of 
      Pae on AP is not clear. In the present study, we aimed to investigate the 
      protective effect and mechanism of Pae on AP in vivo and vitro. In the 
      caerulein-induced mild acute pancreatitis (MAP) model, we found that Pae 
      administration reduced serum levels of amylase, lipase, IL-1beta and IL-6 and 
      alleviated the histopathological manifestations of pancreatic tissue in a 
      dose-dependent manner. And Pae decrease the ROS generated, restore mitochondrial 
      membrane potential (DeltaPsim), inhibit M1 macrophage polarization and NLRP3 
      inflammasome in bone marrow-derived macrophages (BMDMs) in vitro. In addition, 
      specific NLRP3 inhibitor MCC950 eliminated the protective effect of Pae on AP 
      induced by caerulein in mice. Correspondingly, the inhibitory effect of Pae on 
      ROS generated and M1 polarization was not observed in BMDMs with MCC950 in vitro. 
      Taken together, our datas for the first time confirmed the protective effects of 
      Pae on AP via the NLRP3 inflammasomes Pathway.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Yuan, Chenchen
AU  - Yuan C
AD  - Pancreatic Center, Department of Gastroenterology, Affiliated Hospital of 
      Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China; Yangzhou Key 
      Laboratory of Pancreatic Disease, Institute of Digestive Diseases, The Affiliated 
      Hospital of Yangzhou University, Yangzhou University, China.
FAU - Xu, Xingmeng
AU  - Xu X
AD  - Pancreatic Center, Department of Gastroenterology, Affiliated Hospital of 
      Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China; Yangzhou Key 
      Laboratory of Pancreatic Disease, Institute of Digestive Diseases, The Affiliated 
      Hospital of Yangzhou University, Yangzhou University, China.
FAU - Wang, Ningzhi
AU  - Wang N
AD  - Pancreatic Center, Department of Gastroenterology, Affiliated Hospital of 
      Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China; Yangzhou Key 
      Laboratory of Pancreatic Disease, Institute of Digestive Diseases, The Affiliated 
      Hospital of Yangzhou University, Yangzhou University, China.
FAU - Zhu, Qingtian
AU  - Zhu Q
AD  - Pancreatic Center, Department of Gastroenterology, Affiliated Hospital of 
      Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China; Yangzhou Key 
      Laboratory of Pancreatic Disease, Institute of Digestive Diseases, The Affiliated 
      Hospital of Yangzhou University, Yangzhou University, China.
FAU - Zhang, Junxian
AU  - Zhang J
AD  - Pancreatic Center, Department of Gastroenterology, Affiliated Hospital of 
      Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China; Yangzhou Key 
      Laboratory of Pancreatic Disease, Institute of Digestive Diseases, The Affiliated 
      Hospital of Yangzhou University, Yangzhou University, China.
FAU - Gong, Weijuan
AU  - Gong W
AD  - Pancreatic Center, Department of Gastroenterology, Affiliated Hospital of 
      Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China; Yangzhou Key 
      Laboratory of Pancreatic Disease, Institute of Digestive Diseases, The Affiliated 
      Hospital of Yangzhou University, Yangzhou University, China.
FAU - Ding, Yanbing
AU  - Ding Y
AD  - Pancreatic Center, Department of Gastroenterology, Affiliated Hospital of 
      Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China; Yangzhou Key 
      Laboratory of Pancreatic Disease, Institute of Digestive Diseases, The Affiliated 
      Hospital of Yangzhou University, Yangzhou University, China.
FAU - Xiao, Weiming
AU  - Xiao W
AD  - Pancreatic Center, Department of Gastroenterology, Affiliated Hospital of 
      Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China; Yangzhou Key 
      Laboratory of Pancreatic Disease, Institute of Digestive Diseases, The Affiliated 
      Hospital of Yangzhou University, Yangzhou University, China.
FAU - Chen, Weiwei
AU  - Chen W
AD  - Department of Gastroenterology, Clinical Medical College, Yangzhou University, 
      Yangzhou, Jiangsu, China.
FAU - Lu, Guotao
AU  - Lu G
AD  - Pancreatic Center, Department of Gastroenterology, Affiliated Hospital of 
      Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China; Yangzhou Key 
      Laboratory of Pancreatic Disease, Institute of Digestive Diseases, The Affiliated 
      Hospital of Yangzhou University, Yangzhou University, China.
FAU - Yao, Guanghuai
AU  - Yao G
AD  - Pancreatic Center, Department of Gastroenterology, Affiliated Hospital of 
      Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China; Yangzhou Key 
      Laboratory of Pancreatic Disease, Institute of Digestive Diseases, The Affiliated 
      Hospital of Yangzhou University, Yangzhou University, China. Electronic address: 
      ghyao@yzu.edu.cn.
FAU - Pan, Jiajia
AU  - Pan J
AD  - Yangzhou Key Laboratory of Pancreatic Disease, Institute of Digestive Diseases, 
      The Affiliated Hospital of Yangzhou University, Yangzhou University, China; 
      Department of Intensive Care Unit, Affiliated Hospital of Yangzhou University, 
      Yangzhou University, Yangzhou, China. Electronic address: jjpan@yzu.edu.cn.
FAU - Wu, Keyan
AU  - Wu K
AD  - Pancreatic Center, Department of Gastroenterology, Affiliated Hospital of 
      Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, China; Yangzhou Key 
      Laboratory of Pancreatic Disease, Institute of Digestive Diseases, The Affiliated 
      Hospital of Yangzhou University, Yangzhou University, China. Electronic address: 
      kywu@yzu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220208
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Acetophenones)
RN  - 0 (Inflammasomes)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Nlrp3 protein, mouse)
RN  - 0 (Reactive Oxygen Species)
RN  - 3R834EPI82 (paeonol)
RN  - 888Y08971B (Ceruletide)
SB  - IM
MH  - Acetophenones
MH  - Acute Disease
MH  - Animals
MH  - Ceruletide/pharmacology
MH  - *Inflammasomes/metabolism
MH  - Macrophages/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
MH  - *Pancreatitis/chemically induced/drug therapy
MH  - Reactive Oxygen Species/adverse effects
OTO - NOTNLM
OT  - Acute pancreatitis
OT  - Inflammation
OT  - MCC950
OT  - Macrophage polarization
OT  - NLRP3 inflammasomes
OT  - Paeonol
COIS- Declaration of competing interest All authors of this paper have no conflict of 
      interests to disclose.
EDAT- 2022/02/20 06:00
MHDA- 2022/04/12 06:00
CRDT- 2022/02/19 20:14
PHST- 2021/12/14 00:00 [received]
PHST- 2022/02/04 00:00 [revised]
PHST- 2022/02/07 00:00 [accepted]
PHST- 2022/02/20 06:00 [pubmed]
PHST- 2022/04/12 06:00 [medline]
PHST- 2022/02/19 20:14 [entrez]
AID - S0006-291X(22)00201-7 [pii]
AID - 10.1016/j.bbrc.2022.02.019 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2022 Apr 16;600:35-43. doi: 
      10.1016/j.bbrc.2022.02.019. Epub 2022 Feb 8.