PMID- 35185283
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230202
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 74
IP  - 1
DP  - 2022 Feb
TI  - hUC-MSCs: evaluation of acute and long-term routine toxicity testing in mice and 
      rats.
PG  - 17-29
LID - 10.1007/s10616-021-00502-2 [doi]
AB  - Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) are present in 
      human umbilical connective tissue and can differentiate into various cell types. 
      Our previous studies have proved that hUC-MSCs do not lead to allergies and 
      tumorigenesis. In the present study, the acute and long-term toxicity of hUC-MSCs 
      in mice and rats was evaluated. The acute toxicity of hUC-MSCs was assessed in 
      8-week-old mice receiving two caudal intravenous (i.v.) injections of hUC-MSCs at 
      the maximum tolerated dose of 1.5 x 10(7) cells/kg with an interval of 8 h and 
      the observation period sustained for 14 days. For the long-term toxicity 
      evaluation, rats were randomly divided into control, low-dose (3.0 x 10(5) 
      cells/kg), mid-dose (1.5 x 10(6) cells/kg), and high-dose (7.5 x 10(6) cells/kg) 
      groups, which were treated with hUC-MSCs via a caudal i.v. injection every 3 days 
      for 90 days. Weight and food intake evaluation was performed for all rats for 
      2 weeks after the hUC-MSC administration. The animals were then sacrificed for 
      hematological, blood biochemical, and pathological analyses, as well as organ 
      index determination. We observed no obvious acute toxicity of hUC-MSCs in mice at 
      the maximum tolerated dose. Long-term toxicity tests in rats showed no 
      significant differences between HUC-MSC-treated and control groups in the 
      following parameters: body weight, hematological and blood biochemical 
      parameters, and histopathologic changes in the heart, liver, kidneys, and lungs. 
      This study provides evidence of the safety of i.v. hUC-MSCs infusion for future 
      clinical therapies.
CI  - (c) The Author(s), under exclusive licence to Springer Nature B.V. 2022.
FAU - Xu, Jianwei
AU  - Xu J
AD  - National Guizhou Joint Engineering Laboratory for Cell Engineering and 
      Biomedicine Technique, Center for Tissue Engineering and Stem Cell Research, 
      Guizhou Province Key Laboratory of Regenerative Medicine, Guizhou Medical 
      University, Guiyang, China. GRID: grid.413458.f. ISNI: 0000 0000 9330 9891
AD  - Key Laboratory of Adult Stem Cell Translational Research, Chinese Academy of 
      Medical Sciences, Guiyang, China.
AD  - Department of Pharmacology, School of Basic Medicine, Guizhou Medical University, 
      Guiyang, China. GRID: grid.413458.f. ISNI: 0000 0000 9330 9891
FAU - Liu, Gang
AU  - Liu G
AD  - Department of Pharmacology, School of Basic Medicine, Guizhou Medical University, 
      Guiyang, China. GRID: grid.413458.f. ISNI: 0000 0000 9330 9891
FAU - Wang, Xianyao
AU  - Wang X
AD  - National Guizhou Joint Engineering Laboratory for Cell Engineering and 
      Biomedicine Technique, Center for Tissue Engineering and Stem Cell Research, 
      Guizhou Province Key Laboratory of Regenerative Medicine, Guizhou Medical 
      University, Guiyang, China. GRID: grid.413458.f. ISNI: 0000 0000 9330 9891
FAU - Hu, Ya'nan
AU  - Hu Y
AD  - Department of Cell Biology, Medical College of Soochow University, Suzhou, China. 
      GRID: grid.263761.7. ISNI: 0000 0001 0198 0694
FAU - Luo, Hongyang
AU  - Luo H
AD  - Department of Otorhinolaryngology, People's Hospital of Wudang District, Guiyang, 
      China.
FAU - Ye, Lan
AU  - Ye L
AD  - Department of Pharmacology, School of Basic Medicine, Guizhou Medical University, 
      Guiyang, China. GRID: grid.413458.f. ISNI: 0000 0000 9330 9891
FAU - Feng, Zhanhui
AU  - Feng Z
AD  - Neurological Department, Affiliated Hospital of Guizhou Medical University, 
      Guiyang, China. GRID: grid.452244.1
FAU - Li, Chen
AU  - Li C
AD  - Department of Oncology, General Hospital of the Yangtze River Shipping, Wuhan, 
      China.
FAU - Kuang, Menglan
AU  - Kuang M
AD  - School of Pharmacy, Guizhou Medical University, Guiyang, China. GRID: 
      grid.413458.f. ISNI: 0000 0000 9330 9891
FAU - Zhang, Lijuan
AU  - Zhang L
AD  - School of Nursing, Guizhou Medical University, Guiyang, China. GRID: 
      grid.413458.f. ISNI: 0000 0000 9330 9891
FAU - Zhou, Yixia
AU  - Zhou Y
AD  - School of Nursing, Guizhou University of Traditional Chinese Medicine, 9# Beijing 
      Road, Guiyang, China. GRID: grid.443382.a. ISNI: 0000 0004 1804 268X
AD  - Department of Nursing, The Affiliated Hospital of Guizhou Medical University, 
      Guiyang, China. GRID: grid.452244.1
FAU - Qi, Xiaolan
AU  - Qi X
AD  - Key Laboratory of Endemic and Ethnic Diseases (Guizhou Medical University), 
      Ministry of Education, 9# Beijing Road, Guiyang, People's Republic of China. 
      GRID: grid.413458.f. ISNI: 0000 0000 9330 9891
AD  - Key Laboratory of Medical Molecular Biology (Guizhou Medical University), 
      Guiyang, 550004 People's Republic of China. GRID: grid.413458.f. ISNI: 0000 0000 
      9330 9891
LA  - eng
PT  - Journal Article
DEP - 20220105
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC8817012
OTO - NOTNLM
OT  - Acute toxicity
OT  - Biotransformation and toxicokinetics
OT  - Human umbilical cord-derived mesenchymal stem cells
OT  - Long-term toxicity
OT  - Safety evaluation
COIS- Conflict of interestThe authors declare no potential conflicts of interest,All 
      authors state that they have nothing to disclose.
EDAT- 2022/02/22 06:00
MHDA- 2022/02/22 06:01
PMCR- 2023/02/01
CRDT- 2022/02/21 05:59
PHST- 2021/05/21 00:00 [received]
PHST- 2021/10/17 00:00 [accepted]
PHST- 2022/02/21 05:59 [entrez]
PHST- 2022/02/22 06:00 [pubmed]
PHST- 2022/02/22 06:01 [medline]
PHST- 2023/02/01 00:00 [pmc-release]
AID - 502 [pii]
AID - 10.1007/s10616-021-00502-2 [doi]
PST - ppublish
SO  - Cytotechnology. 2022 Feb;74(1):17-29. doi: 10.1007/s10616-021-00502-2. Epub 2022 
      Jan 5.