PMID- 35187647
OWN - NLM
STAT- MEDLINE
DCOM- 20220816
LR  - 20221025
IS  - 1873-3468 (Electronic)
IS  - 0014-5793 (Linking)
VI  - 596
IP  - 15
DP  - 2022 Aug
TI  - Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three 
      novel dual feruloyl/acetyl xylan esterases.
PG  - 1932-1943
LID - 10.1002/1873-3468.14322 [doi]
AB  - Feruloyl esterases (FAEs) and acetyl xylan esterases (AXEs) are important enzymes 
      for plant biomass degradation and are both present in Carbohydrate Esterase 
      family 1 (CE1) of the Carbohydrate-Active enZymes database. In this study, ten 
      novel fungal CE1 enzymes from different subfamilies were heterologously produced 
      and screened for their activity towards model and complex plant biomass 
      substrates. CE1_1 enzymes possess AXE activity, while CE1_5 enzymes showed FAE 
      activity. Two enzymes from CE1_2 and one from CE1_5 possess dual feruloyl/acetyl 
      xylan esterase (FXE) activity, showing expansion of substrate specificity. The 
      new FXEs from CE1 can efficiently release both feruloyl and acetyl residues from 
      feruloylated xylan, making them particularly interesting novel components of 
      industrial enzyme cocktails for plant biomass degradation.
CI  - (c) 2022 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of 
      Federation of European Biochemical Societies.
FAU - Dilokpimol, Adiphol
AU  - Dilokpimol A
AD  - Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular 
      Physiology, Utrecht University, The Netherlands.
FAU - Verkerk, Bart
AU  - Verkerk B
AD  - Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular 
      Physiology, Utrecht University, The Netherlands.
FAU - Li, Xinxin
AU  - Li X
AD  - Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular 
      Physiology, Utrecht University, The Netherlands.
FAU - Bellemare, Annie
AU  - Bellemare A
AD  - Centre for Functional and Structural Genomics, Concordia University, Montreal, 
      Canada.
FAU - Lavallee, Mathieu
AU  - Lavallee M
AD  - Centre for Functional and Structural Genomics, Concordia University, Montreal, 
      Canada.
FAU - Frommhagen, Matthias
AU  - Frommhagen M
AD  - Laboratory of Food Chemistry, Wageningen University and Research, The 
      Netherlands.
FAU - Underlin, Emilie Normolle
AU  - Underlin EN
AD  - Laboratory of Food Chemistry, Wageningen University and Research, The 
      Netherlands.
AD  - Department of Chemistry, Technical University of Denmark, Lyngby, Denmark.
FAU - Kabel, Mirjam A
AU  - Kabel MA
AD  - Laboratory of Food Chemistry, Wageningen University and Research, The 
      Netherlands.
FAU - Powlowski, Justin
AU  - Powlowski J
AD  - Centre for Functional and Structural Genomics, Concordia University, Montreal, 
      Canada.
FAU - Tsang, Adrian
AU  - Tsang A
AD  - Centre for Functional and Structural Genomics, Concordia University, Montreal, 
      Canada.
FAU - de Vries, Ronald P
AU  - de Vries RP
AUID- ORCID: 0000-0002-4363-1123
AD  - Fungal Physiology, Westerdijk Fungal Biodiversity Institute & Fungal Molecular 
      Physiology, Utrecht University, The Netherlands.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220228
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Xylans)
RN  - EC 3.1.- (Esterases)
RN  - EC 3.1.1.- (Carboxylic Ester Hydrolases)
RN  - EC 3.1.1.6 (Acetylesterase)
RN  - EC 3.1.1.72 (acetylxylan esterase)
SB  - IM
MH  - *Acetylesterase/chemistry
MH  - Carboxylic Ester Hydrolases/chemistry
MH  - Esterases/genetics/metabolism
MH  - Substrate Specificity
MH  - *Xylans/metabolism
OTO - NOTNLM
OT  - acetyl xylan esterase
OT  - carbohydrate esterase family 1
OT  - feruloyl esterase
OT  - fungi
OT  - hydroxycinnamic acid
OT  - plant biomass
EDAT- 2022/02/22 06:00
MHDA- 2022/08/17 06:00
CRDT- 2022/02/21 06:08
PHST- 2022/02/09 00:00 [revised]
PHST- 2021/12/27 00:00 [received]
PHST- 2022/02/10 00:00 [accepted]
PHST- 2022/02/22 06:00 [pubmed]
PHST- 2022/08/17 06:00 [medline]
PHST- 2022/02/21 06:08 [entrez]
AID - 10.1002/1873-3468.14322 [doi]
PST - ppublish
SO  - FEBS Lett. 2022 Aug;596(15):1932-1943. doi: 10.1002/1873-3468.14322. Epub 2022 
      Feb 28.