PMID- 35188137
OWN - NLM
STAT- MEDLINE
DCOM- 20220407
LR  - 20220407
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 180
DP  - 2022 Feb 1
TI  - Pneumatically Driven Microfluidic Platform for Micro-Particle Concentration.
LID - 10.3791/63301 [doi]
AB  - The present article introduces a method for fabricating and operating a pneumatic 
      valve to control particle concentration using a microfluidic platform. This 
      platform has a three-dimensional (3D) network with curved fluid channels and 
      three pneumatic valves, which create networks, channels, and spaces through 
      duplex replication with polydimethylsiloxane (PDMS). The device operates based on 
      the transient response of a fluid flow rate controlled by a pneumatic valve in 
      the following order: (1) sample loading, (2) sample blocking, (3) sample 
      concentration, and (4) sample release. The particles are blocked by thin 
      diaphragm layer deformation of the sieve valve (Vs) plate and accumulate in the 
      curved microfluidic channel. The working fluid is discharged by the actuation of 
      two on/off valves. As a result of the operation, all particles of various 
      magnifications were successfully intercepted and disengaged. When this technology 
      is applied, the operating pressure, the time required for concentration, and the 
      concentration rate may vary depending on the device dimensions and particle size 
      magnification.
FAU - Choi, Hong Jin
AU  - Choi HJ
AD  - Department of Digital Anti-Aging Health Care, Inje University.
FAU - Lee, Jong Hyun
AU  - Lee JH
AD  - Micro device Lab Co., Ltd.
FAU - Jeong, Ok Chan
AU  - Jeong OC
AD  - Department of Digital Anti-Aging Health Care, Inje University; Micro device Lab 
      Co., Ltd; Department of Biomedical Engineering, Inje University; 
      memsoku@inje.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20220201
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
SB  - IM
MH  - *Microfluidic Analytical Techniques/methods
MH  - *Microfluidics
MH  - Particle Size
EDAT- 2022/02/22 06:00
MHDA- 2022/04/08 06:00
CRDT- 2022/02/21 08:44
PHST- 2022/02/21 08:44 [entrez]
PHST- 2022/02/22 06:00 [pubmed]
PHST- 2022/04/08 06:00 [medline]
AID - 10.3791/63301 [doi]
PST - epublish
SO  - J Vis Exp. 2022 Feb 1;(180). doi: 10.3791/63301.