PMID- 35188419
OWN - NLM
STAT- MEDLINE
DCOM- 20220901
LR  - 20220906
IS  - 0191-2917 (Print)
IS  - 0191-2917 (Linking)
VI  - 106
IP  - 9
DP  - 2022 Sep
TI  - A Novel Wheat-Rye 2R (2D) Disomic Substitution Line Pyramids Two Types of 
      Resistance to Powdery Mildew.
PG  - 2433-2440
LID - 10.1094/PDIS-12-21-2765-RE [doi]
AB  - Powdery mildew, caused by Blumeria graminis f. sp. tritici, is a devastating 
      disease of wheat that seriously affects yield and quality worldwide. Because of 
      the extensive growth of wheat cultivars with homogeneous genetic background, 
      exploring novel resistant resources from wheat relatives has become important for 
      increasing the genetic diversity of wheat. Rye (Secale cereale) is a wheat 
      relative possessing abundant resistance genes because of its high variation. 
      Wheat line AL69, resistant to powdery mildew, was developed by crossing, 
      backcrossing, and self-pollination for multiple generations between hexaploid 
      triticale Zhongsi 237 and common wheat cultivar Zimai 17. Through genomic in situ 
      hybridization (GISH) and multicolor fluorescence in situ hybridization (FISH), 
      nondenaturing FISH, multicolor GISH, and selection with specific molecular 
      markers, AL69 was determined to be a wheat-rye 2R (2D) disomic substitution line. 
      Testing with different B. graminis f. sp. tritici isolates and genetic analysis 
      showed that the all-stage resistance (also called seedling resistance) of AL69 
      was conferred by the cataloged powdery mildew resistance gene Pm4b derived from 
      Zimai 17, and its adult-plant resistance was derived from the alien chromosome 2R 
      of Zhongsi 237, which was found to be different from the previously reported 
      rye-derived Pm genes, including Pm7 on 2RL. In addition, AL69 showed improved 
      spike number per plant, spike length, fertile spikelet number per spike, kernel 
      number per spike, and grain yield per plant compared with its wheat parent Zimai 
      17. An elite line S251 combining powdery mildew resistance with excellent 
      agronomic performance was selected from the progenies of AL69 and wheat cultivar 
      Jimai 22. Therefore, AL69 has two types of resistance genes to powdery mildew and 
      improved agronomic traits through pyramiding and thus can be used as a promising 
      genetic stock for wheat breeding.
FAU - Han, Guohao
AU  - Han G
AD  - Center for Agricultural Resources Research, Institute of Genetics and 
      Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, Hebei 050022, 
      China.
FAU - Li, Hongwei
AU  - Li H
AD  - The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of 
      Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, 
      China.
FAU - Cao, Lijun
AU  - Cao L
AD  - Center for Agricultural Resources Research, Institute of Genetics and 
      Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, Hebei 050022, 
      China.
FAU - Liu, Shiyu
AU  - Liu S
AD  - Center for Agricultural Resources Research, Institute of Genetics and 
      Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, Hebei 050022, 
      China.
FAU - Yan, Hanwen
AU  - Yan H
AD  - Center for Agricultural Resources Research, Institute of Genetics and 
      Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, Hebei 050022, 
      China.
FAU - Wang, Jing
AU  - Wang J
AD  - Center for Agricultural Resources Research, Institute of Genetics and 
      Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, Hebei 050022, 
      China.
FAU - Zhou, Yilin
AU  - Zhou Y
AD  - The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute 
      of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, 
      China.
FAU - An, Diaoguo
AU  - An D
AUID- ORCID: 0000-0003-2767-6521
AD  - Center for Agricultural Resources Research, Institute of Genetics and 
      Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, Hebei 050022, 
      China.
AD  - The Innovative Academy for Seed Design, Chinese Academy of Sciences, Beijing 
      100101, China.
LA  - eng
PT  - Journal Article
DEP - 20220619
PL  - United States
TA  - Plant Dis
JT  - Plant disease
JID - 9882809
SB  - IM
MH  - Chromosomes, Plant/genetics
MH  - Disease Resistance/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Plant Breeding
MH  - Plant Diseases/genetics
MH  - *Secale/genetics
MH  - *Triticum/genetics
OTO - NOTNLM
OT  - Secale cereale
OT  - Triticum aestivum
OT  - adult-plant resistance
OT  - gene pyramiding
OT  - powdery mildew
OT  - substitution line
EDAT- 2022/02/22 06:00
MHDA- 2022/09/02 06:00
CRDT- 2022/02/21 12:10
PHST- 2022/02/22 06:00 [pubmed]
PHST- 2022/09/02 06:00 [medline]
PHST- 2022/02/21 12:10 [entrez]
AID - 10.1094/PDIS-12-21-2765-RE [doi]
PST - ppublish
SO  - Plant Dis. 2022 Sep;106(9):2433-2440. doi: 10.1094/PDIS-12-21-2765-RE. Epub 2022 
      Jun 19.