PMID- 35189277
OWN - NLM
STAT- MEDLINE
DCOM- 20220315
LR  - 20220315
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 164
DP  - 2022 Mar
TI  - Endotoxin tolerance induced by Porphyromonas gingivalis lipopolysaccharide alters 
      macrophage polarization.
PG  - 105448
LID - S0882-4010(22)00061-4 [pii]
LID - 10.1016/j.micpath.2022.105448 [doi]
AB  - Endotoxin tolerance refers to a state refractory to subsequent lipopolysaccharide 
      (LPS) stimulations following a primary LPS exposure. To study the relationship 
      between endotoxin tolerance and macrophage polarization, endotoxin tolerance was 
      induced by 1 mug/mL LPS from the periodontal pathogen, Porphyromonas gingivalis 
      (P. gingivalis), in peritoneal macrophages (PMs) and bone marrow-derived 
      macrophages (BMDMs). Repeated P. gingivalis LPS challenges increased the 
      quantities of CD206+ PMs, while the number of CD86+CD206+ PMs was reduced 
      compared with the non-tolerant group (p < 0.05). However, there were no changes 
      in BMDMs (p > 0.05). Down regulations of TNF-alpha, IL-12, nitric oxide and MMP-2 
      production, and upregulated IL-10, MMP-9 levels and arginase-1 activities 
      occurred in tolerant PMs and BMDMs (p < 0.05). P. gingivalis LPS-tolerant PMs and 
      BMDMs also enhanced scrape-wound healing abilities of 15p-1 cells (p < 0.05). 
      Expressions of phospho-signal transducer and activator of transcription 6 
      (p-STAT6) and protein tyrosine phosphatase 1B (PTP1B) were increased, while 
      p-MEK1/2 levels were downregulated in tolerant PMs and BMDMs (p < 0.05). IL-10 
      production in tolerant Stat6 knockdown RAW264.7 cells was lower than tolerant 
      control cells (p < 0.05). P. gingivalis LPS-tolerant macrophages represented an 
      intermediate state between M1/M2 polarization, which functioned as M2-like cells, 
      and led to limited inflammatory responses and enhanced wound healing activities. 
      The PTP1B-MEK1/2-STAT6 signaling pathway might be involved in the polarization of 
      tolerant macrophages.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Gu, Jian-Yu
AU  - Gu JY
AD  - Department of Periodontology, The Affiliated Stomatological Hospital of Nanjing 
      Medical University, Nanjing, China; Jiangsu Province Key Laboratory of Oral 
      Diseases, Nanjing, China; Jiangsu Province Engineering Research Center of 
      Stomatological Translational Medicine, Nanjing, China.
FAU - Fu, Zi-Bo
AU  - Fu ZB
AD  - Department of Periodontology, The Affiliated Stomatological Hospital of Nanjing 
      Medical University, Nanjing, China; Jiangsu Province Key Laboratory of Oral 
      Diseases, Nanjing, China; Jiangsu Province Engineering Research Center of 
      Stomatological Translational Medicine, Nanjing, China.
FAU - Jia-Lu Chen
AU  - Jia-Lu Chen
AD  - Department of Periodontology, The Affiliated Stomatological Hospital of Nanjing 
      Medical University, Nanjing, China; Jiangsu Province Key Laboratory of Oral 
      Diseases, Nanjing, China; Jiangsu Province Engineering Research Center of 
      Stomatological Translational Medicine, Nanjing, China.
FAU - Liu, Yu-Jie
AU  - Liu YJ
AD  - Department of Periodontology, The Affiliated Stomatological Hospital of Nanjing 
      Medical University, Nanjing, China; Jiangsu Province Key Laboratory of Oral 
      Diseases, Nanjing, China; Jiangsu Province Engineering Research Center of 
      Stomatological Translational Medicine, Nanjing, China.
FAU - Cao, Xian-Zi
AU  - Cao XZ
AD  - Department of Periodontology, The Affiliated Stomatological Hospital of Nanjing 
      Medical University, Nanjing, China; Jiangsu Province Key Laboratory of Oral 
      Diseases, Nanjing, China; Jiangsu Province Engineering Research Center of 
      Stomatological Translational Medicine, Nanjing, China.
FAU - Sun, Ying
AU  - Sun Y
AD  - Department of Periodontology, The Affiliated Stomatological Hospital of Nanjing 
      Medical University, Nanjing, China; Jiangsu Province Key Laboratory of Oral 
      Diseases, Nanjing, China; Jiangsu Province Engineering Research Center of 
      Stomatological Translational Medicine, Nanjing, China. Electronic address: 
      ebolasun@njmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220218
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Endotoxin Tolerance
MH  - *Lipopolysaccharides/metabolism
MH  - Macrophage Activation
MH  - Macrophages/metabolism
MH  - *Porphyromonas gingivalis
OTO - NOTNLM
OT  - Porphyromonas gingivalis
OT  - endotoxin tolerance
OT  - lipopolysaccharide
OT  - macrophage polarization
EDAT- 2022/02/22 06:00
MHDA- 2022/03/16 06:00
CRDT- 2022/02/21 20:10
PHST- 2021/08/14 00:00 [received]
PHST- 2022/01/10 00:00 [revised]
PHST- 2022/02/14 00:00 [accepted]
PHST- 2022/02/22 06:00 [pubmed]
PHST- 2022/03/16 06:00 [medline]
PHST- 2022/02/21 20:10 [entrez]
AID - S0882-4010(22)00061-4 [pii]
AID - 10.1016/j.micpath.2022.105448 [doi]
PST - ppublish
SO  - Microb Pathog. 2022 Mar;164:105448. doi: 10.1016/j.micpath.2022.105448. Epub 2022 
      Feb 18.