PMID- 35189540
OWN - NLM
STAT- MEDLINE
DCOM- 20220502
LR  - 20220502
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Linking)
VI  - 227
IP  - 2
DP  - 2022 Mar
TI  - CD14 regulates the metabolomic profiles of distinct macrophage subsets under 
      steady and activated states.
PG  - 152191
LID - S0171-2985(22)00017-1 [pii]
LID - 10.1016/j.imbio.2022.152191 [doi]
AB  - Macrophages play pivotal roles during homeostasis and inflammation. They sense 
      exogenous and endogenous molecular patterns via surface and intracellular 
      receptors, which trigger innate immune responses. CD14 is a co-receptor for 
      lipopolysaccharide (LPS), but also drives macrophage responses to Tityus 
      serrulatus scorpion venom (TsV). Cellular activation is tightly coupled with 
      metabolism that sustain their polarization and generate antimicrobial and 
      signaling molecules. Macrophage's origin and nature of stimulus are critical for 
      their responses, but whether these factors impact macrophage metabolism is 
      unknown. Moreover, the regulation of intracellular metabolism by CD14 has not 
      been assessed. Using an untargeted metabolomics approach, we determined the 
      longitudinal metabolic responses of peritoneal (PMs) and bone marrow derived 
      macrophages (BMDMs) stimulated with LPS and TsV for 12 h. These data revealed 
      alterations on the relative levels of several metabolites and pathways related to 
      amino acids, nucleotides, lipids, and vitamins. Our data suggest activation of 
      selenoamino acid metabolism and increased abundance of selenomethionine in both 
      cell subsets stimulated with LPS. Moreover, the results suggest a differential 
      activity of vitamin B3 metabolism pathway in response to TsV stimulus, with 
      differences on regulation of the relative levels of nicotinamide mononucleotide 
      and deamino-NAD(+). CD14 deficiency affects the metabolome of both cell subsets 
      at steady state. Moreover, CD14 was required for arginine consumption in PMs 
      stimulated with LPS, but not TsV or by BMDMs stimulated by both stimuli. 
      Importantly, the data suggest that CD14 mediates the accumulation of lipids in 
      both macrophage subsets stimulated with LPS, providing insights into the 
      potential role of CD14 for the development of metabolic diseases. We conclude 
      that macrophages acquire a spectrum of metabolic profiles that depend on the 
      origin of these cells, the nature of the stimuli and signaling by innate immune 
      receptors.
CI  - Copyright (c) 2022 Elsevier GmbH. All rights reserved.
FAU - de Macedo, Luana Henrique
AU  - de Macedo LH
AD  - Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de 
      Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao 
      Preto, SP, Brazil.
FAU - Souza, Camila Oliveira Silva
AU  - Souza COS
AD  - Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de 
      Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao 
      Preto, SP, Brazil.
FAU - Gardinassi, Luiz Gustavo
AU  - Gardinassi LG
AD  - Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de 
      Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao 
      Preto, SP, Brazil; Departamento de Biociencias e Tecnologia, Instituto de 
      Patologia Tropical e Saude Publica, Universidade Federal de Goias, Goiania, GO, 
      Brazil. Electronic address: luizgardinassi@ufg.br.
FAU - Faccioli, Lucia Helena
AU  - Faccioli LH
AD  - Departamento de Analises Clinicas, Toxicologicas e Bromatologicas, Faculdade de 
      Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao 
      Preto, SP, Brazil. Electronic address: faccioli@fcfrp.usp.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220217
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Scorpion Venoms)
SB  - IM
MH  - Lipopolysaccharide Receptors/metabolism
MH  - *Lipopolysaccharides/metabolism
MH  - Macrophages
MH  - Metabolomics
MH  - *Scorpion Venoms/metabolism
MH  - Signal Transduction
OTO - NOTNLM
OT  - CD14
OT  - Homeostasis
OT  - Inflammation
OT  - Macrophages
OT  - Metabolomics
EDAT- 2022/02/22 06:00
MHDA- 2022/05/03 06:00
CRDT- 2022/02/21 20:13
PHST- 2021/10/16 00:00 [received]
PHST- 2022/01/28 00:00 [revised]
PHST- 2022/02/14 00:00 [accepted]
PHST- 2022/02/22 06:00 [pubmed]
PHST- 2022/05/03 06:00 [medline]
PHST- 2022/02/21 20:13 [entrez]
AID - S0171-2985(22)00017-1 [pii]
AID - 10.1016/j.imbio.2022.152191 [doi]
PST - ppublish
SO  - Immunobiology. 2022 Mar;227(2):152191. doi: 10.1016/j.imbio.2022.152191. Epub 
      2022 Feb 17.