PMID- 35190756
OWN - NLM
STAT- MEDLINE
DCOM- 20220328
LR  - 20220501
IS  - 1875-8630 (Electronic)
IS  - 0278-0240 (Print)
IS  - 0278-0240 (Linking)
VI  - 2022
DP  - 2022
TI  - LC-MS-Based Lipidomic Analysis of Serum Samples from Patients with Type 2 
      Diabetes Mellitus (T2DM).
PG  - 5559470
LID - 10.1155/2022/5559470 [doi]
LID - 5559470
AB  - BACKGROUND: With the development of social economy, type 2 diabetes mellitus 
      (T2DM) is becoming a severe health problem globally. METHODS: To systematically 
      understand the lipid metabolism in T2DM, we applied untargeted lipidomics to the 
      serum of T2DM patients and control group using ultrahigh-performance liquid 
      chromatography (UHPLC) coupled with high-resolution mass spectrometry (MS). 
      RESULTS: Over two thousand molecular features were detected by our approach, of 
      which 222 lipid species in positive ion mode and 145 species in negative were 
      reliably identified based on precise molecular weights and MS/MS patterns. 
      Multivariate analysis was adopted to differentiate T2DM patients and the control 
      group using principal component analysis (PCA) and orthogonal partial least 
      squares discrimination analysis (OPLS-DA). The dysregulated lipid species were 
      found and their significance in pathophysiology was discussed. Correlation 
      analysis of selected lipids and important clinical variables was performed and 
      addressed. CONCLUSIONS: This study unveils several new lipids and pathways 
      considerably involved in T2DM and provides novel insights into understanding the 
      pathogenesis underlying T2DM.
CI  - Copyright (c) 2022 Jia Liu et al.
FAU - Liu, Jia
AU  - Liu J
AUID- ORCID: 0000-0002-2845-1028
AD  - Institute of Systems Biomedicine, Department of Pathology, School of Basic 
      Medical Sciences, Beijing Key Laboratory of Tumor Systems Biology, 
      Peking-Tsinghua Center for Life Sciences, Peking University Health Science 
      Center, Beijing 100191, China.
AD  - The First Affiliated Hospital of Hebei North University, Zhangjiakou, Hebei 
      075000, China.
FAU - Bai, Lu
AU  - Bai L
AD  - Department of Cardiology, Beijing Ditan Hospital, Capital Medical University, 
      Beijing 100015, China.
FAU - Wang, Weimin
AU  - Wang W
AD  - Department of Cardiology, Peking University People's Hospital, Beijing 100044, 
      China.
FAU - Song, Yuqing
AU  - Song Y
AD  - Department of Cardiology, Beijing Ditan Hospital, Capital Medical University, 
      Beijing 100015, China.
FAU - Zhao, Wenbo
AU  - Zhao W
AD  - State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China.
FAU - Li, Qingwei
AU  - Li Q
AUID- ORCID: 0000-0002-3887-6759
AD  - State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China.
FAU - Wu, Qiming
AU  - Wu Q
AUID- ORCID: 0000-0002-3464-1098
AD  - Department of Cardiology, Beijing Ditan Hospital, Capital Medical University, 
      Beijing 100015, China.
LA  - eng
PT  - Journal Article
DEP - 20220212
PL  - United States
TA  - Dis Markers
JT  - Disease markers
JID - 8604127
RN  - 0 (Biomarkers)
RN  - 0 (Lipids)
SB  - IM
MH  - Aged
MH  - Biomarkers/blood
MH  - *Chromatography, High Pressure Liquid
MH  - Diabetes Mellitus, Type 2/*blood
MH  - Dyslipidemias/diagnosis
MH  - Female
MH  - Humans
MH  - Least-Squares Analysis
MH  - Lipidomics
MH  - Lipids/*blood
MH  - Male
MH  - Middle Aged
MH  - Principal Component Analysis
MH  - *Tandem Mass Spectrometry
PMC - PMC8858047
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2022/02/23 06:00
MHDA- 2022/03/29 06:00
PMCR- 2022/02/12
CRDT- 2022/02/22 06:08
PHST- 2021/02/19 00:00 [received]
PHST- 2021/10/28 00:00 [revised]
PHST- 2022/01/19 00:00 [accepted]
PHST- 2022/02/22 06:08 [entrez]
PHST- 2022/02/23 06:00 [pubmed]
PHST- 2022/03/29 06:00 [medline]
PHST- 2022/02/12 00:00 [pmc-release]
AID - 10.1155/2022/5559470 [doi]
PST - epublish
SO  - Dis Markers. 2022 Feb 12;2022:5559470. doi: 10.1155/2022/5559470. eCollection 
      2022.