PMID- 35190857
OWN - NLM
STAT- MEDLINE
DCOM- 20230228
LR  - 20230228
IS  - 1432-184X (Electronic)
IS  - 0095-3628 (Linking)
VI  - 85
IP  - 2
DP  - 2023 Feb
TI  - Biosynthesis of Calcite Nanocrystal by a Novel Polyextremophile Bhargavaea 
      cecembensis-Related Strain Isolated from Sandy Soil.
PG  - 698-707
LID - 10.1007/s00248-022-01977-y [doi]
AB  - Urease-producing bacteria are abundant in soils, which can precipitate calcium 
      carbonate nanocrystals by enzymatic hydrolysis of urea in the presence of calcium 
      ions. This process is known as microbially induced calcium carbonate 
      precipitation (MICP), and it has received much attention in recent years as an 
      eco-friendly technology. Therefore, the purpose of the present study was to 
      isolate local extremophile bacterial strains capable of producing calcium 
      carbonate. Among a total of 44 isolated urease-producing strains from sandy 
      soils, one strain with a high level of urease activity (8.16 U/ml) and production 
      of a large amount of calcium carbonate (410 mg/100 ml) was selected for further 
      investigation. 16S rRNA gene sequencing showed that this strain had 99.66% 
      sequence identity to Bhargavaea cecembensis. The SEM-EDX and XRD analyses 
      indicated that irregular vaterite and aggregated nanocalcite were the dominant 
      polymorphs produced by this strain. The size of these nanocalcite crystals ranged 
      between 25 and 42 nm. The selected strain showed high levels of tolerance to 
      different conditions of temperature, pH, and salinity. This strain grows at high 
      temperatures up to 50  degrees C, alkaline pH (9-11), and high concentrations of NaCl 
      (20-25% w/v). Flow cytometry analysis demonstrated 96% cell viability of the 
      isolated strain after desiccation stress. Bhargavaea was first reported in 2009 
      as a new genus, and it belongs to the Firmicutes. So far, there has been no 
      report on its MICP potential. The present study is the first one to report 
      nanocrystal calcium carbonate precipitation in polyextremophile Bhargavaea 
      cecembensis, which makes it a suitable candidate for bio-cementation under 
      extreme circumstances.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Elmi, Fatemeh
AU  - Elmi F
AD  - Department of Cell and Molecular Biology & Microbiology, Faculty of Biological 
      Science and Technology, University of Isfahan, Isfahan, 8174673441, Iran.
FAU - Etemadifar, Zahra
AU  - Etemadifar Z
AUID- ORCID: 0000-0003-1480-9663
AD  - Department of Cell and Molecular Biology & Microbiology, Faculty of Biological 
      Science and Technology, University of Isfahan, Isfahan, 8174673441, Iran. 
      z.etemadifar@sci.ui.ac.ir.
FAU - Emtiazi, Giti
AU  - Emtiazi G
AD  - Department of Cell and Molecular Biology & Microbiology, Faculty of Biological 
      Science and Technology, University of Isfahan, Isfahan, 8174673441, Iran.
LA  - eng
PT  - Journal Article
DEP - 20220221
PL  - United States
TA  - Microb Ecol
JT  - Microbial ecology
JID - 7500663
RN  - 0 (Sand)
RN  - 0 (Soil)
RN  - H0G9379FGK (Calcium Carbonate)
RN  - EC 3.5.1.5 (Urease)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - Bhargavaea cecembensis
SB  - IM
MH  - *Sand
MH  - Soil
MH  - Calcium Carbonate/chemistry
MH  - Urease
MH  - RNA, Ribosomal, 16S/genetics
MH  - Bacteria/genetics
MH  - *Nanoparticles
MH  - Chemical Precipitation
OTO - NOTNLM
OT  - Bhargavaea cecembensis
OT  - Nanocalcite
OT  - Polyextremophile
OT  - Precipitation
EDAT- 2022/02/23 06:00
MHDA- 2023/03/03 06:00
CRDT- 2022/02/22 06:08
PHST- 2021/08/23 00:00 [received]
PHST- 2022/01/28 00:00 [accepted]
PHST- 2022/02/23 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2022/02/22 06:08 [entrez]
AID - 10.1007/s00248-022-01977-y [pii]
AID - 10.1007/s00248-022-01977-y [doi]
PST - ppublish
SO  - Microb Ecol. 2023 Feb;85(2):698-707. doi: 10.1007/s00248-022-01977-y. Epub 2022 
      Feb 21.