PMID- 35190967
OWN - NLM
STAT- MEDLINE
DCOM- 20220823
LR  - 20220829
IS  - 2629-3277 (Electronic)
IS  - 2629-3269 (Print)
IS  - 2629-3277 (Linking)
VI  - 18
IP  - 6
DP  - 2022 Aug
TI  - The miR151 and miR5100 Transfected Bone Marrow Stromal Cells Increase Myoblast 
      Fusion in IGFBP2 Dependent Manner.
PG  - 2164-2178
LID - 10.1007/s12015-022-10350-y [doi]
AB  - BACKGROUND: Bone marrow stromal cells (BMSCs) form a perivascular cell population 
      in the bone marrow. These cells do not present naive myogenic potential. However, 
      their myogenic identity could be induced experimentally in vitro or in vivo. In 
      vivo, after transplantation into injured muscle, BMSCs rarely fused with 
      myofibers. However, BMSC participation in myofiber reconstruction increased if 
      they were modified by NICD or PAX3 overexpression. Nevertheless, BMSCs paracrine 
      function could play a positive role in skeletal muscle regeneration. Previously, 
      we showed that SDF-1 treatment and coculture with myofibers increased BMSC 
      ability to reconstruct myofibers. We also noticed that SDF-1 treatment changed 
      selected miRNAs expression, including miR151 and miR5100. METHODS: Mouse BMSCs 
      were transfected with miR151 and miR5100 mimics and their proliferation, myogenic 
      differentiation, and fusion with myoblasts were analyzed. RESULTS: We showed that 
      miR151 and miR5100 played an important role in the regulation of BMSC 
      proliferation and migration. Moreover, the presence of miR151 and miR5100 
      transfected BMSCs in co-cultures with human myoblasts increased their fusion. 
      This effect was achieved in an IGFBP2 dependent manner. CONCLUSIONS: Mouse BMSCs 
      did not present naive myogenic potential but secreted proteins could impact 
      myogenic cell differentiation. miR151 and miR5100 transfection changed BMSC 
      migration and IGFBP2 and MMP12 expression in BMSCs. miR151 and miR5100 
      transfected BMSCs increased myoblast fusion in vitro.
CI  - (c) 2022. The Author(s).
FAU - Mierzejewski, Bartosz
AU  - Mierzejewski B
AD  - Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1 
      St, 02-096, Warszawa, Poland.
FAU - Michalska, Zuzanna
AU  - Michalska Z
AD  - Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1 
      St, 02-096, Warszawa, Poland.
FAU - Jackowski, Daniel
AU  - Jackowski D
AD  - Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1 
      St, 02-096, Warszawa, Poland.
FAU - Streminska, Wladyslawa
AU  - Streminska W
AD  - Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1 
      St, 02-096, Warszawa, Poland.
FAU - Janczyk-Ilach, Katarzyna
AU  - Janczyk-Ilach K
AD  - Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1 
      St, 02-096, Warszawa, Poland.
FAU - Koblowska, Marta
AU  - Koblowska M
AD  - Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-096, 
      Warsaw, Poland.
AD  - Laboratory of Microarray Analysis, Institute of Biochemistry and Biophysics, 
      Polish Academy of Sciences, 02-106, Warsaw, Poland.
FAU - Iwanicka-Nowicka, Roksana
AU  - Iwanicka-Nowicka R
AD  - Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, 02-096, 
      Warsaw, Poland.
AD  - Laboratory of Microarray Analysis, Institute of Biochemistry and Biophysics, 
      Polish Academy of Sciences, 02-106, Warsaw, Poland.
FAU - Gromadka, Agnieszka
AU  - Gromadka A
AD  - Department of Bioinformatics, Institute of Biochemistry and Biophysics, Polish 
      Academy of Sciences, 02-106, Warsaw, Poland.
FAU - Ciemerych, Maria Anna
AU  - Ciemerych MA
AD  - Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1 
      St, 02-096, Warszawa, Poland.
FAU - Brzoska, Edyta
AU  - Brzoska E
AUID- ORCID: 0000-0002-7886-0436
AD  - Department of Cytology, Faculty of Biology, University of Warsaw, Miecznikowa 1 
      St, 02-096, Warszawa, Poland. edbrzoska@biol.uw.edu.pl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220221
PL  - United States
TA  - Stem Cell Rev Rep
JT  - Stem cell reviews and reports
JID - 101752767
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - Cell Differentiation/genetics
MH  - Humans
MH  - *Mesenchymal Stem Cell Transplantation
MH  - *Mesenchymal Stem Cells
MH  - Mice
MH  - Myoblasts
PMC - PMC9391248
OTO - NOTNLM
OT  - SDF-1
OT  - bone marrow-derived mesenchymal stem/stromal cells
OT  - fusion
OT  - miR151
OT  - miR5100
OT  - migration
OT  - myoblasts
OT  - proliferation
COIS- The Authors declare no conflict of interest.
EDAT- 2022/02/23 06:00
MHDA- 2022/08/24 06:00
PMCR- 2022/02/21
CRDT- 2022/02/22 06:09
PHST- 2022/01/31 00:00 [accepted]
PHST- 2022/02/23 06:00 [pubmed]
PHST- 2022/08/24 06:00 [medline]
PHST- 2022/02/22 06:09 [entrez]
PHST- 2022/02/21 00:00 [pmc-release]
AID - 10.1007/s12015-022-10350-y [pii]
AID - 10350 [pii]
AID - 10.1007/s12015-022-10350-y [doi]
PST - ppublish
SO  - Stem Cell Rev Rep. 2022 Aug;18(6):2164-2178. doi: 10.1007/s12015-022-10350-y. 
      Epub 2022 Feb 21.