PMID- 35192720
OWN - NLM
STAT- MEDLINE
DCOM- 20220608
LR  - 20220610
IS  - 1460-2377 (Electronic)
IS  - 0953-8178 (Linking)
VI  - 34
IP  - 6
DP  - 2022 Jun 4
TI  - In silico analyses and experimental validation of the MHC class-I restricted 
      epitopes of Ebolavirus GP.
PG  - 313-325
LID - 10.1093/intimm/dxac006 [doi]
AB  - Ebolavirus (EBOV) causes an extremely high mortality and prevalence disease 
      called Ebola virus disease (EVD). There is only one glycoprotein (GP) on the 
      virus particle surface, which mediates entry into the host cell. Major 
      histocompatibility complex (MHC) class-I restricted cluster of differentiation 8 
      (CD8+) T cell responses are important antiviral immune responses. Therefore, it 
      is of great importance to understand EBOV GP-specific MHC class-I restricted 
      epitopes within immunogenicity. In this study, computational approaches were 
      employed to predict the dominant MHC class-I molecule epitopes of EBOV GP for 
      mouse H2 and major alleles of human leukocyte antigen (HLA) class-I supertypes. 
      Our results yielded 42 dominant epitopes in H2 haplotypes and 301 dominant 
      epitopes in HLA class-I haplotypes. After validation by enzyme-linked immunospot 
      (ELISpot) assay, in-depth analyses to ascertain their nature of conservation, 
      immunogenicity, and docking with the corresponding MHC class-I molecules were 
      undertaken. Our study predicted MHC class-I restricted epitopes that may aid the 
      advancement of anti-EBOV immune responses. An integrated strategy of epitope 
      prediction, validation and comparative analyses was postulated, which is 
      promising for epitope-based immunotherapy development and application to viral 
      epidemics.
CI  - (c) The Author(s) 2022. Published by Oxford University Press on behalf of The 
      Japanese Society for Immunology. All rights reserved. For permissions, please 
      e-mail: journals.permissions@oup.com.
FAU - Liu, Yang
AU  - Liu Y
AD  - Department of Immunology, Basic Medicine School, Air-Force Medical University 
      (the Fourth Military Medical University), Xi'an, Shaanxi, 86-710032, P.R. China.
AD  - Institute of AIDS Prevention and Control，Shaanxi Provincial Center for Disease 
      Control and Prevention, Xi'an, Shaanxi, 86-710054, P.R. China.
FAU - Sun, Baozeng
AU  - Sun B
AD  - Department of Immunology, Basic Medicine School, Air-Force Medical University 
      (the Fourth Military Medical University), Xi'an, Shaanxi, 86-710032, P.R. China.
FAU - Wang, Jiawei
AU  - Wang J
AD  - Department of Immunology, Basic Medicine School, Air-Force Medical University 
      (the Fourth Military Medical University), Xi'an, Shaanxi, 86-710032, P.R. China.
FAU - Sun, Hao
AU  - Sun H
AD  - Department of Immunology, Basic Medicine School, Air-Force Medical University 
      (the Fourth Military Medical University), Xi'an, Shaanxi, 86-710032, P.R. China.
AD  - Department of Internal Medicine, Tangshan Sannvhe Airport Hospital, Tangshan, 
      Hebei, 86-063000, P.R. China.
FAU - Lu, Zhenhua
AU  - Lu Z
AD  - Department of Epidemiology, Public Health School, Air-Force Medical University 
      (the Fourth Military Medical University), Xi'an, Shaanxi, 86-710032, P.R. China.
FAU - Chen, Longyu
AU  - Chen L
AD  - Department of Immunology, Basic Medicine School, Air-Force Medical University 
      (the Fourth Military Medical University), Xi'an, Shaanxi, 86-710032, P.R. China.
FAU - Lan, Mingfu
AU  - Lan M
AD  - Department of Immunology, Basic Medicine School, Air-Force Medical University 
      (the Fourth Military Medical University), Xi'an, Shaanxi, 86-710032, P.R. China.
FAU - Xu, Jiahao
AU  - Xu J
AD  - Department of Immunology, Basic Medicine School, Air-Force Medical University 
      (the Fourth Military Medical University), Xi'an, Shaanxi, 86-710032, P.R. China.
FAU - Pan, Jingyu
AU  - Pan J
AD  - Department of Immunology, Basic Medicine School, Air-Force Medical University 
      (the Fourth Military Medical University), Xi'an, Shaanxi, 86-710032, P.R. China.
FAU - Shi, Jingqi
AU  - Shi J
AD  - Department of Immunology, Basic Medicine School, Air-Force Medical University 
      (the Fourth Military Medical University), Xi'an, Shaanxi, 86-710032, P.R. China.
FAU - Sun, Yuanjie
AU  - Sun Y
AD  - Department of Immunology, Basic Medicine School, Air-Force Medical University 
      (the Fourth Military Medical University), Xi'an, Shaanxi, 86-710032, P.R. China.
FAU - Zhang, Xiyang
AU  - Zhang X
AD  - Department of Immunology, Basic Medicine School, Air-Force Medical University 
      (the Fourth Military Medical University), Xi'an, Shaanxi, 86-710032, P.R. China.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Immunology, Basic Medicine School, Air-Force Medical University 
      (the Fourth Military Medical University), Xi'an, Shaanxi, 86-710032, P.R. China.
FAU - Jiang, Dongbo
AU  - Jiang D
AUID- ORCID: 0000-0002-2675-5634
AD  - Department of Immunology, Basic Medicine School, Air-Force Medical University 
      (the Fourth Military Medical University), Xi'an, Shaanxi, 86-710032, P.R. China.
FAU - Yang, Kun
AU  - Yang K
AD  - Department of Immunology, Basic Medicine School, Air-Force Medical University 
      (the Fourth Military Medical University), Xi'an, Shaanxi, 86-710032, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Glycoproteins)
RN  - 0 (HLA Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Animals
MH  - *Ebolavirus
MH  - Epitopes, T-Lymphocyte
MH  - Glycoproteins
MH  - HLA Antigens
MH  - Histocompatibility Antigens Class I
MH  - Mice
OTO - NOTNLM
OT  - Ebolavirus (EBOV)
OT  - in silico analyse
OT  - MHC class-I epitope
OT  - glycoprotein (GP)
OT  - molecular docking
EDAT- 2022/02/23 06:00
MHDA- 2022/06/09 06:00
CRDT- 2022/02/22 17:13
PHST- 2021/07/16 00:00 [received]
PHST- 2022/02/22 00:00 [accepted]
PHST- 2022/02/23 06:00 [pubmed]
PHST- 2022/06/09 06:00 [medline]
PHST- 2022/02/22 17:13 [entrez]
AID - 6534472 [pii]
AID - 10.1093/intimm/dxac006 [doi]
PST - ppublish
SO  - Int Immunol. 2022 Jun 4;34(6):313-325. doi: 10.1093/intimm/dxac006.