PMID- 35196626
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231020
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1193
DP  - 2022 Mar 15
TI  - Direct determination of nosiheptide residue in animal tissues by liquid 
      chromatography-tandem mass spectrometry.
PG  - 123167
LID - S1570-0232(22)00071-X [pii]
LID - 10.1016/j.jchromb.2022.123167 [doi]
AB  - Because only very weak signals of fragment ions of nosiheptide can be obtained, 
      nosiheptide is usually detected by liquid chromatography-tandem mass spectrometry 
      (LC-MS/MS) via the determination of its hydrolyzed degradation product named HMIA 
      in previous studies. The indirect method suffers from several problems, such as 
      complicated samplepreparation, unavailable commercial HMIA, and the risk of the 
      false-positive result by HMIA. However, we found that nosiheptide could produce 
      several significant fragment ions under high collision energy (CE). Therefore, we 
      developed a method for the direct determination of nosiheptide by LC-MS/MS in 
      animal tissues. The sample was extracted with ACN, then degreased with n-hexane, 
      and purified by an HLB solid-phase extraction (SPE) cartridge. After being 
      filtered through the PTFE filter, it was analyzed by LC-MS/MS in selected 
      reaction monitoring (SRM) mode. The influencing factors, such as mobile phase, 
      SPE cartridge, filter material, and matrix effect, were investigated. Nosiheptide 
      showed a good linear relationship (R(2) >/= 0.999) within the concentration range 
      from 0.3 mug/L to 20 mug/L under optimized conditions. The limit of detection (LOD) 
      was 0.3 mug/kg, while the limit of quantification (LOQ) was 1.0 mug/kg in chicken, 
      bovine muscle, swine muscle, and swine liver. The average recoveries at spiked 
      levels of 1.0, 2.0, and 10 mug/kg ranged from 83% to 101%, with the relative 
      standard deviations (RSDs) <12%. Compared with the methods previously reported, 
      our newly developed method was more simple, convenient, and sensitive. Moreover, 
      it was successfully applied for the determination of nosiheptide residue in 
      medicated chicken samples.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Shen, Jincan
AU  - Shen J
AD  - Food Inspection and Quarantine Technology Center of Shenzhen Customs District, 
      Shenzhen 518045, China.
FAU - Zhao, Fengjuan
AU  - Zhao F
AD  - Food Inspection and Quarantine Technology Center of Shenzhen Customs District, 
      Shenzhen 518045, China.
FAU - Zhu, Pingping
AU  - Zhu P
AD  - Food Inspection and Quarantine Technology Center of Shenzhen Customs District, 
      Shenzhen 518045, China.
FAU - Wu, Fengqi
AU  - Wu F
AD  - Food Inspection and Quarantine Technology Center of Shenzhen Customs District, 
      Shenzhen 518045, China.
FAU - Chen, Xinyi
AU  - Chen X
AD  - Food Inspection and Quarantine Technology Center of Shenzhen Customs District, 
      Shenzhen 518045, China.
FAU - Kang, Haining
AU  - Kang H
AD  - Food Inspection and Quarantine Technology Center of Shenzhen Customs District, 
      Shenzhen 518045, China.
FAU - Yue, Zhenfeng
AU  - Yue Z
AD  - Food Inspection and Quarantine Technology Center of Shenzhen Customs District, 
      Shenzhen 518045, China; Shenzhen Polytechnic, Shenzhen 518045, China. Electronic 
      address: yuezhenfeng@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220217
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
SB  - IM
OTO - NOTNLM
OT  - Animal tissues
OT  - Direct determination
OT  - LC-MS/MS
OT  - Nosiheptide
OT  - Residue
EDAT- 2022/02/24 06:00
MHDA- 2022/02/24 06:01
CRDT- 2022/02/23 20:09
PHST- 2021/10/18 00:00 [received]
PHST- 2021/12/28 00:00 [revised]
PHST- 2022/02/07 00:00 [accepted]
PHST- 2022/02/24 06:01 [medline]
PHST- 2022/02/24 06:00 [pubmed]
PHST- 2022/02/23 20:09 [entrez]
AID - S1570-0232(22)00071-X [pii]
AID - 10.1016/j.jchromb.2022.123167 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2022 Mar 15;1193:123167. doi: 
      10.1016/j.jchromb.2022.123167. Epub 2022 Feb 17.