PMID- 35198589 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220225 IS - 2296-861X (Print) IS - 2296-861X (Electronic) IS - 2296-861X (Linking) VI - 9 DP - 2022 TI - A Competitive Assay Based on Dual-Mode Au@Pt-DNA Biosensors for On-Site Sensitive Determination of Carbendazim Fungicide in Agricultural Products. PG - 820150 LID - 10.3389/fnut.2022.820150 [doi] LID - 820150 AB - Carbendazim (CBZ), a systemic, broad-spectrum benzimidazole fungicide, is widely used to control fungal diseases in agricultural products. Its residues might pose risks to human health and the environment. Therefore, it is warranted to establish a rapid and reliable method for its residual quantification. Herein, we proposed a competitive assay that combined aptamer (DNA) specific recognition and bimetallic nanozyme gold@platinum (Au@Pt) catalysis to trace the CBZ residue. The DNA was labeled onto bimetallic nanozyme Au@Pt surface to produce Au@Pt probes (Au@Pt-DNA). The magnetic Fe(3)O(4) was functionalized with a complementary strand of DNA (C-DNA) to form Fe(3)O(4) probes (Fe(3)O(4)-C-DNA). Subsequently, the CBZ and the Fe(3)O(4) probes competitively react with Au@Pt probes to form two Au@Pt-DNA biosensors (Au@Pt-ssDNA-CBZ and Au@Pt-dsDNA-Fe(3)O(4)). The Au@Pt-ssDNA-CBZ biosensor was designed for qualitative analysis through a naked-eye visualization strategy in the presence of CBZ. Meanwhile, Au@Pt-dsDNA-Fe(3)O(4) biosensor was developed to quantitatively analyze CBZ using a multifunctional microplate reader. A competitive assay based on the dual-mode Au@Pt-DNA biosensors was established for onsite sensitive determination of CBZ. The limit of detection (LOD) and recoveries of the developed assay were 0.038 ng/mg and 71.88-110.11%, with relative standard deviations (RSDs) ranging between 3.15 and 10.91%. The assay demonstrated a good correlation with data acquired from liquid chromatography coupled with mass spectrometry/mass spectrometry analysis. In summary, the proposed competitive assay based on dual-mode Au@Pt-DNA biosensors might have a great potential for onsite sensitive detection of pesticides in agro-products. CI - Copyright (c) 2022 Chen, Zhai, Liu, Huang, Zhang, Xu, Li, Zhang, Wang, Jin, Xu and Abd El-Aty. FAU - Chen, Ge AU - Chen G AD - Key Laboratory of Vegetables Quality and Safety Control, Laboratory of Quality and Safety Risk Assessment for Vegetable Products, Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China. FAU - Zhai, Rongqi AU - Zhai R AD - Key Laboratory of Vegetables Quality and Safety Control, Laboratory of Quality and Safety Risk Assessment for Vegetable Products, Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China. FAU - Liu, Guangyang AU - Liu G AD - Key Laboratory of Vegetables Quality and Safety Control, Laboratory of Quality and Safety Risk Assessment for Vegetable Products, Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China. FAU - Huang, Xiaodong AU - Huang X AD - Key Laboratory of Vegetables Quality and Safety Control, Laboratory of Quality and Safety Risk Assessment for Vegetable Products, Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China. FAU - Zhang, Kaige AU - Zhang K AD - Key Laboratory of Vegetables Quality and Safety Control, Laboratory of Quality and Safety Risk Assessment for Vegetable Products, Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China. FAU - Xu, Xiaomin AU - Xu X AD - Key Laboratory of Vegetables Quality and Safety Control, Laboratory of Quality and Safety Risk Assessment for Vegetable Products, Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China. FAU - Li, Lingyun AU - Li L AD - Key Laboratory of Vegetables Quality and Safety Control, Laboratory of Quality and Safety Risk Assessment for Vegetable Products, Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China. FAU - Zhang, Yanguo AU - Zhang Y AD - Key Laboratory of Vegetables Quality and Safety Control, Laboratory of Quality and Safety Risk Assessment for Vegetable Products, Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China. FAU - Wang, Jing AU - Wang J AD - Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture and Rural Affairs, Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, China. FAU - Jin, Maojun AU - Jin M AD - Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture and Rural Affairs, Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, China. FAU - Xu, Donghui AU - Xu D AD - Key Laboratory of Vegetables Quality and Safety Control, Laboratory of Quality and Safety Risk Assessment for Vegetable Products, Ministry of Agriculture and Rural Affairs, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China. FAU - Abd El-Aty, A M AU - Abd El-Aty AM AD - State Key Laboratory of Biobased Material and Green Papermaking, College of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Science, Jinan, China. AD - Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt. AD - Department of Medical Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey. LA - eng PT - Journal Article DEP - 20220207 PL - Switzerland TA - Front Nutr JT - Frontiers in nutrition JID - 101642264 PMC - PMC8860170 OTO - NOTNLM OT - Au@Pt-DNA OT - aptamer OT - biosensor OT - carbendazim OT - pesticide residue COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2022/02/25 06:00 MHDA- 2022/02/25 06:01 PMCR- 2022/01/01 CRDT- 2022/02/24 05:43 PHST- 2021/11/22 00:00 [received] PHST- 2022/01/03 00:00 [accepted] PHST- 2022/02/24 05:43 [entrez] PHST- 2022/02/25 06:00 [pubmed] PHST- 2022/02/25 06:01 [medline] PHST- 2022/01/01 00:00 [pmc-release] AID - 10.3389/fnut.2022.820150 [doi] PST - epublish SO - Front Nutr. 2022 Feb 7;9:820150. doi: 10.3389/fnut.2022.820150. eCollection 2022.