PMID- 35200617
OWN - NLM
STAT- MEDLINE
DCOM- 20220317
LR  - 20220317
IS  - 1660-3397 (Electronic)
IS  - 1660-3397 (Linking)
VI  - 20
IP  - 2
DP  - 2022 Jan 20
TI  - Metabolites Produced by a New Lactiplantibacillus plantarum Strain BF1-13 
      Isolated from Deep Seawater of Izu-Akazawa Protect the Intestinal Epithelial 
      Barrier from the Dysfunction Induced by Hydrogen Peroxide.
LID - 10.3390/md20020087 [doi]
LID - 87
AB  - This study aimed to investigate the protective effect of the metabolites produced 
      by a new Lactiplantibacillus plantarum strain BF1-13, isolated from deep seawater 
      (DSW), on the intestinal epithelial barrier against the dysfunction induced by 
      hydrogen peroxide (H(2)O(2)) and to elucidate the mechanism underlying the 
      effect. Protective effect of the metabolites by strain BF1-13 on the barrier 
      function of the intestinal epithelial model treated with H(2)O(2) was 
      investigated by the transepithelial electrical resistance (TEER). The metabolites 
      enhanced the Claudin-4 (CLDN-4) expression, including at the transcription level, 
      indicated by immunofluorescence staining and quantitative RT-PCR. The metabolites 
      also showed a suppression of aquaporin3 (AQP3) expression. Lactic acid (LA) 
      produced by this strain of homofermentative lactic acid bacteria (LAB) had a 
      similar enhancement on CLDN-4 expression. The metabolites of L. plantarum strain 
      BF1-13 alleviated the dysfunction of intestinal epithelial barrier owing to its 
      enhancement on the tight junctions (TJs) by LA, along with its suppression on 
      AQP3-facilitating H(2)O(2) intracellular invasion into Caco-2 cells. This is the 
      first report on the enhancement of TJs by LA produced by LAB.
FAU - Diao, Xiaozhen
AU  - Diao X
AUID- ORCID: 0000-0003-1805-5408
AD  - Applied Microbiology Lab, Course of Applied Marine Biosciences, Graduate School 
      of Marine Science and Technology, Tokyo University of Marine Science and 
      Technology, Tokyo 108-8477, Japan.
FAU - Yamada, Katsuhisa
AU  - Yamada K
AD  - Applied Microbiology Lab, Course of Applied Marine Biosciences, Graduate School 
      of Marine Science and Technology, Tokyo University of Marine Science and 
      Technology, Tokyo 108-8477, Japan.
AD  - DSW Laboratory of DHC Co., Ltd., Tokyo 106-0047, Japan.
FAU - Shibata, Yuji
AU  - Shibata Y
AD  - DSW Laboratory of DHC Co., Ltd., Tokyo 106-0047, Japan.
FAU - Imada, Chiaki
AU  - Imada C
AD  - Applied Microbiology Lab, Course of Applied Marine Biosciences, Graduate School 
      of Marine Science and Technology, Tokyo University of Marine Science and 
      Technology, Tokyo 108-8477, Japan.
LA  - eng
PT  - Journal Article
DEP - 20220120
PL  - Switzerland
TA  - Mar Drugs
JT  - Marine drugs
JID - 101213729
RN  - 0 (AQP3 protein, human)
RN  - 0 (Protective Agents)
RN  - 158801-98-0 (Aquaporin 3)
RN  - 33X04XA5AT (Lactic Acid)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Aquaporin 3/genetics
MH  - Caco-2 Cells
MH  - Humans
MH  - Hydrogen Peroxide/toxicity
MH  - Intestinal Mucosa/*drug effects/pathology
MH  - Lactic Acid/metabolism
MH  - Lactobacillus plantarum/isolation & purification/*metabolism
MH  - Protective Agents/isolation & purification/*pharmacology
MH  - Seawater
MH  - Tight Junctions/drug effects
PMC - PMC8878880
OTO - NOTNLM
OT  - Lactiplantibacillus plantarum
OT  - aquaporin3
OT  - deep seawater
OT  - hydrogen peroxide
OT  - lactic acid
OT  - tight junctions
COIS- The authors declare no conflict of interest.
EDAT- 2022/02/25 06:00
MHDA- 2022/03/18 06:00
PMCR- 2022/01/20
CRDT- 2022/02/24 12:20
PHST- 2021/12/02 00:00 [received]
PHST- 2022/01/14 00:00 [revised]
PHST- 2022/01/18 00:00 [accepted]
PHST- 2022/02/24 12:20 [entrez]
PHST- 2022/02/25 06:00 [pubmed]
PHST- 2022/03/18 06:00 [medline]
PHST- 2022/01/20 00:00 [pmc-release]
AID - md20020087 [pii]
AID - marinedrugs-20-00087 [pii]
AID - 10.3390/md20020087 [doi]
PST - epublish
SO  - Mar Drugs. 2022 Jan 20;20(2):87. doi: 10.3390/md20020087.