PMID- 35202706
OWN - NLM
STAT- MEDLINE
DCOM- 20220427
LR  - 20220506
IS  - 1096-0007 (Electronic)
IS  - 0014-4835 (Linking)
VI  - 218
DP  - 2022 May
TI  - LncRNA MALAT1 aggravates the retinal angiogenesis via miR-320a/HIF-1alpha axis in 
      diabetic retinopathy.
PG  - 108984
LID - S0014-4835(22)00065-3 [pii]
LID - 10.1016/j.exer.2022.108984 [doi]
AB  - Diabetic retinopathy (DR) is one of the most serious microvascular complications 
      of diabetes and an important cause of blindness in adults. In previous study, we 
      found that miR-320a alleviated the damage of muller cells in DR. In this study, 
      we mainly explored the mechanism of lncRNA MALAT1 on retinal angiogenesis in DR 
      by regulating miR-320a/HIF-1alpha. The expression of MALAT1 and miR-320a was detected 
      by RT-qPCR, and the expression of HIF-1alpha was detected by Western blot. The 
      superoxide anion level, invasion, angiogenesis, and vascular permeability of 
      mouse retinal microvascular endothelial cells (MRMECs) co-cultured with muller 
      cells were evaluated by dihydroethidium, transwell, angiogenesis and 
      immunofluorescence assay. In order to analyze the targeting relationship between 
      miR-320a and MALAT1 or HIF-1alpha, we performed dual luciferase reporter gene, 
      fluorescence in situ hybridization (FISH), RNA immunoprecipitation (RIP) and RNA 
      pulldown experiments. The results should that MALAT1 and HIF-1alpha were highly 
      expressed and miR-320a was low expressed in high glucose (HG)-induced muller 
      cells, and MALAT1 could competitively bind with HIF-1alpha. Knocking down miR-320a 
      inhibited MRMECs invasion angiogenesis, and vascular permeability by targeting 
      miR-320a. Overexpression of miR-320a down regulated HIF-1alpha and inhibited the 
      invasion, angiogenesis, and vascular permeability of MRMECs. In conclusion, 
      MALAT1 inhibits HIF-1alpha expression and MRMECs angiogenesis in DR through spongy 
      miR-320a.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Chen, Zhen
AU  - Chen Z
AD  - Department of Ophthalmology, The First People's Hospital of Yunnan Province, 
      Kunming, Yunnan, 650032, China; Medical College of Kunming University of Science 
      and Technology, Kunming, Yunnan, 650500, China; Medical College of Dali 
      University, Dali, Yunnan, 671003, China.
FAU - Yang, Jingying
AU  - Yang J
AD  - Department of Ophthalmology, The First People's Hospital of Yunnan Province, 
      Kunming, Yunnan, 650032, China.
FAU - Gao, Yang
AU  - Gao Y
AD  - Department of Ophthalmology, The First People's Hospital of Yunnan Province, 
      Kunming, Yunnan, 650032, China.
FAU - Jiang, Shanshan
AU  - Jiang S
AD  - Department of Ophthalmology, The First People's Hospital of Yunnan Province, 
      Kunming, Yunnan, 650032, China; Medical College of Dali University, Dali, Yunnan, 
      671003, China.
FAU - Li, Zuyou
AU  - Li Z
AD  - Department of Ophthalmology, The First People's Hospital of Yunnan Province, 
      Kunming, Yunnan, 650032, China; Medical College of Kunming University of Science 
      and Technology, Kunming, Yunnan, 650500, China.
FAU - Wang, Yao
AU  - Wang Y
AD  - Department of Ophthalmology, The First People's Hospital of Yunnan Province, 
      Kunming, Yunnan, 650032, China; Medical College of Kunming University of Science 
      and Technology, Kunming, Yunnan, 650500, China.
FAU - Hu, Zhongyin
AU  - Hu Z
AD  - Department of Ophthalmology, The First People's Hospital of Yunnan Province, 
      Kunming, Yunnan, 650032, China.
FAU - Han, Fang
AU  - Han F
AD  - Department of Ophthalmology, The First People's Hospital of Yunnan Province, 
      Kunming, Yunnan, 650032, China.
FAU - Ni, Ninghua
AU  - Ni N
AD  - Department of Ophthalmology, The First People's Hospital of Yunnan Province, 
      Kunming, Yunnan, 650032, China. Electronic address: nnh1972@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220221
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Animals
MH  - *Diabetes Mellitus/metabolism
MH  - *Diabetic Retinopathy/genetics/metabolism
MH  - Endothelial Cells/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - *MicroRNAs/genetics/metabolism
MH  - Neovascularization, Pathologic/metabolism
MH  - *RNA, Long Noncoding/genetics
OTO - NOTNLM
OT  - Diabetic retinopathy
OT  - MALAT1
OT  - Muller cells
OT  - Retinal angiogenesis
OT  - miR-320a
EDAT- 2022/02/25 06:00
MHDA- 2022/04/28 06:00
CRDT- 2022/02/24 20:11
PHST- 2021/07/01 00:00 [received]
PHST- 2022/01/26 00:00 [revised]
PHST- 2022/02/10 00:00 [accepted]
PHST- 2022/02/25 06:00 [pubmed]
PHST- 2022/04/28 06:00 [medline]
PHST- 2022/02/24 20:11 [entrez]
AID - S0014-4835(22)00065-3 [pii]
AID - 10.1016/j.exer.2022.108984 [doi]
PST - ppublish
SO  - Exp Eye Res. 2022 May;218:108984. doi: 10.1016/j.exer.2022.108984. Epub 2022 Feb 
      21.