PMID- 35202987
OWN - NLM
STAT- MEDLINE
DCOM- 20220506
LR  - 20220506
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 152
DP  - 2022 Apr
TI  - Aqueous extract of Passiflora alata leaves modulates in vitro the indoleamine 
      2,3-dioxygenase (IDO) and CD86 expression in bone marrow-derived professional 
      antigen-presenting cells polarizing NOD mice T cells to a Treg profile.
PG  - 155832
LID - S1043-4666(22)00041-2 [pii]
LID - 10.1016/j.cyto.2022.155832 [doi]
AB  - Dendritic cells (DCs) and macrophages are professional antigen-presenting cells 
      (pAPCs), numerous in the pancreas of nonobese diabetic (NOD) mice and playing an 
      essential role in the autoimmune response of type 1 diabetes. The expression of 
      the enzyme indoleamine 2,3-dioxygenase (IDO) is a critical factor for the 
      tolerogenic activity of pAPCs, acting in the catabolism of tryptophan, providing 
      metabolites that suppress the T cell effectors and induce T regulatory cells 
      differentiation. Here we investigated the in vitro mechanisms of lyophilized 
      aqueous extract from Passiflora alata leaves (LAEPAL) that modulates bone 
      marrow-derived professional antigen-presenting cells (BM-pAPCs), affecting their 
      ability to polarize T cells. A cell culture model was defined using mixed 
      cultures of BM-pAPCs and T lymphocytes NOD mice with stressed MIN-6 cells as a 
      source of pancreatic beta cells antigens. We showed that the treatment with 
      300 microg/mL of LAEPAL induces a significant decrease in the CD4 and CD8 T effector 
      lymphocytes proliferation from diabetic but not in non-diabetic mice, followed by 
      a reduction of the IL-6 and IFN-gamma cytokines release in the cell cultures 
      supernatants. Moreover, we observed an increase of CD4(+)CD25(+)FoxP3(+) Tregs in 
      the cell cultures from diabetic mice. These results could be partially explained 
      by the LAEPAL modulatory effects in BM-pAPCs, downregulating the CD86 
      co-stimulatory molecule expression, and increasing IDO-1 expression in F4/80+ 
      BM-pAPCs. These results contribute to a better understanding of the polyphenols' 
      immunomodulatory properties, meaning they could induce tolerogenic 
      antigen-presenting cells, which could polarize T cells to a Treg profile and 
      decrease the activity of CD4+ and CD8+ T effector cells.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Schumacher, Nayara Simon Gonzalez
AU  - Schumacher NSG
AD  - Laboratory of Translational Immunology, School of Medical Sciences, University of 
      Campinas (UNICAMP), Campinas 13083-888, Brazil. Electronic address: 
      n144325@dac.unicamp.br.
FAU - Fernandes, Luis Gustavo Romani
AU  - Fernandes LGR
AD  - Laboratory of Translational Immunology, School of Medical Sciences, University of 
      Campinas (UNICAMP), Campinas 13083-888, Brazil. Electronic address: 
      luisgrf@unicamp.br.
FAU - de Lima Zollner, Ricardo
AU  - de Lima Zollner R
AD  - Laboratory of Translational Immunology, School of Medical Sciences, University of 
      Campinas (UNICAMP), Campinas 13083-888, Brazil. Electronic address: 
      zollner@unicamp.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220221
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Antigens)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Cd86 protein, mouse)
RN  - 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase)
SB  - IM
MH  - Animals
MH  - Antigen-Presenting Cells/metabolism
MH  - Antigens
MH  - B7-2 Antigen/metabolism
MH  - Bone Marrow/metabolism
MH  - Cells, Cultured
MH  - Dendritic Cells
MH  - *Diabetes Mellitus, Experimental
MH  - Humans
MH  - Indoleamine-Pyrrole 2,3,-Dioxygenase/metabolism
MH  - Mice
MH  - Mice, Inbred NOD
MH  - *Passiflora/metabolism
MH  - Plant Leaves
MH  - T-Lymphocytes, Regulatory
OTO - NOTNLM
OT  - Antigen-presenting cells and Passiflora alata
OT  - NOD mice
OT  - Type 1 diabetes mellitus
EDAT- 2022/02/25 06:00
MHDA- 2022/05/07 06:00
CRDT- 2022/02/24 20:14
PHST- 2021/03/25 00:00 [received]
PHST- 2022/02/03 00:00 [revised]
PHST- 2022/02/15 00:00 [accepted]
PHST- 2022/02/25 06:00 [pubmed]
PHST- 2022/05/07 06:00 [medline]
PHST- 2022/02/24 20:14 [entrez]
AID - S1043-4666(22)00041-2 [pii]
AID - 10.1016/j.cyto.2022.155832 [doi]
PST - ppublish
SO  - Cytokine. 2022 Apr;152:155832. doi: 10.1016/j.cyto.2022.155832. Epub 2022 Feb 21.