PMID- 35203066
OWN - NLM
STAT- MEDLINE
DCOM- 20220415
LR  - 20220415
IS  - 1758-5090 (Electronic)
IS  - 1758-5082 (Linking)
VI  - 14
IP  - 2
DP  - 2022 Mar 9
TI  - All-in-one biofabrication and loading of recombinant vaults in human cells.
LID - 10.1088/1758-5090/ac584d [doi]
AB  - One of the most promising approaches in the drug delivery field is the use of 
      naturally occurring self-assembling protein nanoparticles, such as virus-like 
      particles, bacterial microcompartments or vault ribonucleoprotein particles as 
      drug delivery systems (DDSs). Among them, eukaryotic vaults show a promising 
      future due to their structural features,in vitrostability and non-immunogenicity. 
      Recombinant vaults are routinely produced in insect cells and purified through 
      several ultracentrifugations, both tedious and time-consuming processes. As an 
      alternative, this work proposes a new approach and protocols for the production 
      of recombinant vaults in human cells by transient gene expression of a His-tagged 
      version of the major vault protein (MVP-H6), the development of new 
      affinity-based purification processes for such recombinant vaults, and the 
      all-in-one biofabrication and encapsulation of a cargo recombinant protein within 
      such vaults by their co-expression in human cells. Protocols proposed here allow 
      the easy and straightforward biofabrication and purification of engineered vaults 
      loaded with virtually any INT-tagged cargo protein, in very short times, paving 
      the way to faster and easier engineering and production of better and more 
      efficient DDS.
CI  - Creative Commons Attribution license.
FAU - Martin, Fernando
AU  - Martin F
AUID- ORCID: 0000-0002-3496-9093
AD  - Institut de Biotecnologia i de Biomedicina, Universitat Autonoma de Barcelona, 
      Bellaterra, 08193 Barcelona, Spain.
FAU - Carreno, Aida
AU  - Carreno A
AUID- ORCID: 0000-0001-7725-7515
AD  - Institut de Biotecnologia i de Biomedicina, Universitat Autonoma de Barcelona, 
      Bellaterra, 08193 Barcelona, Spain.
FAU - Mendoza, Rosa
AU  - Mendoza R
AUID- ORCID: 0000-0002-0488-4814
AD  - Institut de Biotecnologia i de Biomedicina, Universitat Autonoma de Barcelona, 
      Bellaterra, 08193 Barcelona, Spain.
AD  - CIBER de Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), Bellaterra, 
      08193 Barcelona, Spain.
FAU - Caruana, Pablo
AU  - Caruana P
AUID- ORCID: 0000-0002-0170-4355
AD  - Grup d Oncologia Ginecologica i Peritoneal. Institut d'Investigacions Biomediques 
      Sant Pau, Hospital de Santa Creu i Sant Pau, 08041 Barcelona, Spain.
FAU - Rodriguez, Francisco
AU  - Rodriguez F
AUID- ORCID: 0000-0001-7909-932X
AD  - Grup d Oncologia Ginecologica i Peritoneal. Institut d'Investigacions Biomediques 
      Sant Pau, Hospital de Santa Creu i Sant Pau, 08041 Barcelona, Spain.
FAU - Bravo, Marlon
AU  - Bravo M
AUID- ORCID: 0000-0002-0065-9812
AD  - Laboratori d'Enginyeria de Proteines, Departament de Biologia, Universitat de 
      Girona, 17003 Girona, Spain.
AD  - Institut d'Investigacio Biomedica de Girona Josep Trueta, (IdIBGi), 17190 Salt, 
      Spain.
FAU - Benito, Antoni
AU  - Benito A
AUID- ORCID: 0000-0002-9776-1003
AD  - Laboratori d'Enginyeria de Proteines, Departament de Biologia, Universitat de 
      Girona, 17003 Girona, Spain.
AD  - Institut d'Investigacio Biomedica de Girona Josep Trueta, (IdIBGi), 17190 Salt, 
      Spain.
FAU - Ferrer-Miralles, Neus
AU  - Ferrer-Miralles N
AUID- ORCID: 0000-0003-2981-3913
AD  - Institut de Biotecnologia i de Biomedicina, Universitat Autonoma de Barcelona, 
      Bellaterra, 08193 Barcelona, Spain.
AD  - CIBER de Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), Bellaterra, 
      08193 Barcelona, Spain.
AD  - Departament de Genetica i de Microbiologia, Universitat Autonoma de Barcelona, 
      Bellaterra, 08193 Barcelona, Spain.
FAU - Cespedes, M Virtudes
AU  - Cespedes MV
AUID- ORCID: 0000-0003-2956-5833
AD  - Grup d Oncologia Ginecologica i Peritoneal. Institut d'Investigacions Biomediques 
      Sant Pau, Hospital de Santa Creu i Sant Pau, 08041 Barcelona, Spain.
FAU - Corchero, Jose Luis
AU  - Corchero JL
AUID- ORCID: 0000-0002-6109-144X
AD  - Institut de Biotecnologia i de Biomedicina, Universitat Autonoma de Barcelona, 
      Bellaterra, 08193 Barcelona, Spain.
AD  - CIBER de Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN), Bellaterra, 
      08193 Barcelona, Spain.
AD  - Departament de Genetica i de Microbiologia, Universitat Autonoma de Barcelona, 
      Bellaterra, 08193 Barcelona, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220309
PL  - England
TA  - Biofabrication
JT  - Biofabrication
JID - 101521964
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Drug Delivery Systems
MH  - Humans
MH  - *Nanoparticles/chemistry
MH  - Recombinant Proteins/chemistry
OTO - NOTNLM
OT  - drug delivery systems
OT  - eukaryotic cell factories
OT  - protein nanocages
OT  - self-assembling protein nanoparticles
OT  - vault particles
EDAT- 2022/02/25 06:00
MHDA- 2022/04/16 06:00
CRDT- 2022/02/24 20:17
PHST- 2021/02/09 00:00 [received]
PHST- 2022/02/24 00:00 [accepted]
PHST- 2022/02/25 06:00 [pubmed]
PHST- 2022/04/16 06:00 [medline]
PHST- 2022/02/24 20:17 [entrez]
AID - 10.1088/1758-5090/ac584d [doi]
PST - epublish
SO  - Biofabrication. 2022 Mar 9;14(2). doi: 10.1088/1758-5090/ac584d.