PMID- 35203427
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220301
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 10
IP  - 2
DP  - 2022 Jan 20
TI  - Association of Lipoproteins with Neutrophil Extracellular Traps in Patients with 
      Abdominal Aortic Aneurysm.
LID - 10.3390/biomedicines10020217 [doi]
LID - 217
AB  - Neutrophil extracellular traps (NETs) are DNA-protein structures released by 
      neutrophils in response to various stimuli, including oxidized, low-density 
      lipoprotein (oxLDL). Accumulating evidence suggests a role for NETs in the 
      pathogenesis of abdominal aortic aneurysm (AAA). In this study, we investigated 
      the potential association of lipoprotein particles and NETs in AAA in comparison 
      to non-AAA control groups. The concentrations of neutrophil myeloperoxidase 
      (MPO), the NET parameters citrullinated histone H3 (citH3) and circulating 
      cell-free DNA (cfDNA), as well as of blood lipids were determined in plasma or 
      serum of patients with AAA (n = 40), peripheral artery occlusive disease (PAD; n 
      = 40) and healthy donors (n = 29). A sandwich ELISA detecting oxidized 
      phosphatidylcholine in association with apolipoprotein B-100 (oxPL/apoB) was 
      applied to measure oxidized phospholipids in circulation. The effect of 
      lipoparticles on NET formation was tested using a DNA release assay with isolated 
      human neutrophils. Plasma MPO, citH3 and cfDNA levels were significantly 
      increased in AAA patients in comparison to healthy donors and PAD patients. 
      Plasma concentrations of citH3 positively correlated with serum oxPL/apoB in AAA 
      patients. In functional in vitro assays, the addition of oxLDL induced NET 
      formation in pre-stimulated neutrophils. In conclusion, our data suggest a 
      promoting role of oxLDL on NET formation in AAA patients.
FAU - Brandau, Annika
AU  - Brandau A
AUID- ORCID: 0000-0003-2947-7809
AD  - Division of Vascular Surgery, Department of General Surgery, Medical University 
      of Vienna, Vienna General Hospital, 1090 Vienna, Austria.
FAU - Ibrahim, Nahla
AU  - Ibrahim N
AUID- ORCID: 0000-0002-2083-9175
AD  - Division of Vascular Surgery, Department of General Surgery, Medical University 
      of Vienna, Vienna General Hospital, 1090 Vienna, Austria.
FAU - Klopf, Johannes
AU  - Klopf J
AUID- ORCID: 0000-0001-9283-5133
AD  - Division of Vascular Surgery, Department of General Surgery, Medical University 
      of Vienna, Vienna General Hospital, 1090 Vienna, Austria.
FAU - Hayden, Hubert
AU  - Hayden H
AD  - Division of Vascular Surgery, Department of General Surgery, Medical University 
      of Vienna, Vienna General Hospital, 1090 Vienna, Austria.
FAU - Ozsvar-Kozma, Maria
AU  - Ozsvar-Kozma M
AD  - Department of Laboratory Medicine, Medical University of Vienna, Vienna General 
      Hospital, 1090 Vienna, Austria.
FAU - Afonyushkin, Taras
AU  - Afonyushkin T
AD  - Department of Laboratory Medicine, Medical University of Vienna, Vienna General 
      Hospital, 1090 Vienna, Austria.
FAU - Bleichert, Sonja
AU  - Bleichert S
AD  - Division of Vascular Surgery, Department of General Surgery, Medical University 
      of Vienna, Vienna General Hospital, 1090 Vienna, Austria.
FAU - Fuchs, Lukas
AU  - Fuchs L
AD  - Division of Vascular Surgery, Department of General Surgery, Medical University 
      of Vienna, Vienna General Hospital, 1090 Vienna, Austria.
FAU - Watzinger, Viktoria
AU  - Watzinger V
AD  - Division of Vascular Surgery, Department of General Surgery, Medical University 
      of Vienna, Vienna General Hospital, 1090 Vienna, Austria.
FAU - Nairz, Verena
AU  - Nairz V
AD  - Division of Vascular Surgery, Department of General Surgery, Medical University 
      of Vienna, Vienna General Hospital, 1090 Vienna, Austria.
FAU - Manville, Emely
AU  - Manville E
AD  - Division of Vascular Surgery, Department of General Surgery, Medical University 
      of Vienna, Vienna General Hospital, 1090 Vienna, Austria.
FAU - Kessler, Veronika
AU  - Kessler V
AD  - Division of Vascular Surgery, Department of General Surgery, Medical University 
      of Vienna, Vienna General Hospital, 1090 Vienna, Austria.
FAU - Stangl, Herbert
AU  - Stangl H
AUID- ORCID: 0000-0002-7288-7320
AD  - Center for Pathobiochemistry and Genetics, Department of Medical Chemistry, 
      Medical University of Vienna, 1090 Vienna, Austria.
FAU - Eilenberg, Wolf
AU  - Eilenberg W
AUID- ORCID: 0000-0002-9322-6193
AD  - Division of Vascular Surgery, Department of General Surgery, Medical University 
      of Vienna, Vienna General Hospital, 1090 Vienna, Austria.
FAU - Neumayer, Christoph
AU  - Neumayer C
AUID- ORCID: 0000-0003-1407-4185
AD  - Division of Vascular Surgery, Department of General Surgery, Medical University 
      of Vienna, Vienna General Hospital, 1090 Vienna, Austria.
FAU - Brostjan, Christine
AU  - Brostjan C
AUID- ORCID: 0000-0003-1462-5397
AD  - Division of Vascular Surgery, Department of General Surgery, Medical University 
      of Vienna, Vienna General Hospital, 1090 Vienna, Austria.
LA  - eng
GR  - F5409-B21/FWF Austrian Science Fund/
PT  - Journal Article
DEP - 20220120
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC8869298
OTO - NOTNLM
OT  - abdominal aortic aneurysm
OT  - citrullinated histones
OT  - lipoproteins
OT  - neutrophil extracellular traps
OT  - peripheral arterial occlusive disease
COIS- The authors declare no conflict of interest.
EDAT- 2022/02/26 06:00
MHDA- 2022/02/26 06:01
PMCR- 2022/01/20
CRDT- 2022/02/25 01:01
PHST- 2021/12/09 00:00 [received]
PHST- 2022/01/15 00:00 [revised]
PHST- 2022/01/17 00:00 [accepted]
PHST- 2022/02/25 01:01 [entrez]
PHST- 2022/02/26 06:00 [pubmed]
PHST- 2022/02/26 06:01 [medline]
PHST- 2022/01/20 00:00 [pmc-release]
AID - biomedicines10020217 [pii]
AID - biomedicines-10-00217 [pii]
AID - 10.3390/biomedicines10020217 [doi]
PST - epublish
SO  - Biomedicines. 2022 Jan 20;10(2):217. doi: 10.3390/biomedicines10020217.