PMID- 35204104
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220228
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 11
IP  - 2
DP  - 2022 Jan 24
TI  - Hyperoside and Quercitrin in Houttuynia cordata Extract Attenuate UVB-Induced 
      Human Keratinocyte Cell Damage and Oxidative Stress via Modulation of MAPKs and 
      Akt Signaling Pathway.
LID - 10.3390/antiox11020221 [doi]
LID - 221
AB  - Ultraviolet radiation is a major environmental harmful factor on human skin. In 
      this paper, we investigate the potential mechanism of Houttuynia cordata extract 
      on UVB-induced HaCaT keratinocyte cell death and inflammation. We found that 
      Houttuynia cordata ethyl acetate extract fraction (HC-EA) protected against 
      UVB-induced cell damage. The HPLC results indicate that quercitrin and hyperoside 
      are the major polyphenolics in HC-EA and are responsible for providing protection 
      against UVB-induced cell death. These responses were associated with the 
      regulation of caspase-9 and caspase-3 activation, which rescued HaCaT cells from 
      UVB-induced apoptosis. In addition, HC-EA, quercitrin, and hyperoside attenuated 
      UVB-induced inflammatory mediators, including IL-6, IL-8, COX-2, and iNOS. 
      Furthermore, the treatment of cells with HC-EA and its active compounds abolished 
      intracellular ROS and increased levels of heme oxygenase-1 and superoxide 
      dismutase. UVB-induced ROS production mediated Akt and mitogen activated protein 
      kinases (MAPKs) pathways, including p38, ERK, and JNK. Our results show HC-EA, 
      quercitrin, and hyperoside decreased UVB-induced p38 and JNK phosphorylation, 
      while increasing ERK and Akt phosphorylation. MAPKs and Akt mediated cell 
      survival and death were confirmed by specific inhibitors to Akt and MAPKs. Thus, 
      HC-EA, which contains quercitrin and hyperoside, protected keratinocyte from 
      UVB-induced oxidative damage and inflammation through the modulation of MAPKs and 
      Akt signaling.
FAU - Charachit, Nattakan
AU  - Charachit N
AD  - Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang 
      Mai 50200, Thailand.
FAU - Sukhamwang, Amonnat
AU  - Sukhamwang A
AD  - Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang 
      Mai 50200, Thailand.
FAU - Dejkriengkraikul, Pornngarm
AU  - Dejkriengkraikul P
AUID- ORCID: 0000-0001-8732-8911
AD  - Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang 
      Mai 50200, Thailand.
AD  - Center for Research and Development of Natural Products for Health, Chiang Mai 
      University, Chiang Mai 50200, Thailand.
FAU - Yodkeeree, Supachai
AU  - Yodkeeree S
AD  - Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang 
      Mai 50200, Thailand.
AD  - Center for Research and Development of Natural Products for Health, Chiang Mai 
      University, Chiang Mai 50200, Thailand.
LA  - eng
GR  - 162/2563/Faculty of Medicine Research Fund/
GR  - 14/2565/the Center for Research and Development of Natural Products for Health/
PT  - Journal Article
DEP - 20220124
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC8868276
OTO - NOTNLM
OT  - HaCaT keratinocyte
OT  - Houttuynia cordata
OT  - UVB
OT  - anti-apoptosis
OT  - antioxidant
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2022/02/26 06:00
MHDA- 2022/02/26 06:01
PMCR- 2022/01/24
CRDT- 2022/02/25 01:03
PHST- 2021/12/31 00:00 [received]
PHST- 2022/01/17 00:00 [revised]
PHST- 2022/01/21 00:00 [accepted]
PHST- 2022/02/25 01:03 [entrez]
PHST- 2022/02/26 06:00 [pubmed]
PHST- 2022/02/26 06:01 [medline]
PHST- 2022/01/24 00:00 [pmc-release]
AID - antiox11020221 [pii]
AID - antioxidants-11-00221 [pii]
AID - 10.3390/antiox11020221 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2022 Jan 24;11(2):221. doi: 10.3390/antiox11020221.