PMID- 35205174
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240326
IS  - 2079-7737 (Print)
IS  - 2079-7737 (Electronic)
IS  - 2079-7737 (Linking)
VI  - 11
IP  - 2
DP  - 2022 Feb 15
TI  - Pb Induces MCP-1 in the Choroid Plexus.
LID - 10.3390/biology11020308 [doi]
LID - 308
AB  - Lead (Pb) is an environmental element that has been implicated in the development 
      of dementia and Alzheimer's disease (AD). Additionally, innate immune activation 
      contributes to AD pathophysiology. However, the mechanisms involved remain poorly 
      understood. The choroid plexus (CP) is not only the site of cerebrospinal fluid 
      (CSF) production, but also an important location for communication between the 
      circulation and the CSF. In this study, we investigated the involvement of the CP 
      during Pb exposure by evaluating the expression of the monocyte chemoattractant 
      protein-1 (MCP-1). MCP-1 is highly expressed in the CP compared to other CNS 
      tissues. MCP-1 regulates macrophage infiltration and is upregulated in AD brains. 
      Our study revealed that Pb exposure stimulated MCP-1 expression, along with a 
      significantly increased macrophage infiltration into the CP. By using cultured 
      Z310 rat CP cells, Pb exposure stimulated MCP-1 expression in a dose-related 
      fashion and markedly activated both NF-kappaB and p38 MAP kinase. Interestingly, both 
      SB 203580, a p38 inhibitor, and BAY 11-7082, an NF-kappaB p65 inhibitor, 
      significantly blocked Pb-induced MCP-1 expression. However, SB203580 did not 
      directly inhibit NF-kappaB p65 phosphorylation. In conclusion, Pb exposure stimulates 
      MCP-1 expression via the p38 and NF-kappaB p65 pathways along with macrophage 
      infiltration into the CP.
FAU - Gu, Huiying
AU  - Gu H
AD  - Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 
      46202, USA.
FAU - Xu, Yundan
AU  - Xu Y
AD  - Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 
      46202, USA.
AD  - School of Basic Medical Science, Hubei University of Chinese Medicine, Wuhan 
      430065, China.
FAU - Du, Nicole
AU  - Du N
AD  - Department of Pediatrics, Children's National Hospital, Washington, DC 20010, 
      USA.
FAU - Yu, Yongqi
AU  - Yu Y
AD  - Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 
      46202, USA.
FAU - Zheng, Wei
AU  - Zheng W
AD  - School of Health Sciences, Purdue University, West Lafayette, IN 47907, USA.
FAU - Du, Yansheng
AU  - Du Y
AD  - Department of Neurology, Indiana University School of Medicine, Indianapolis, IN 
      46202, USA.
LA  - eng
GR  - R01 ES027078/ES/NIEHS NIH HHS/United States
GR  - NIH/NIA R21 AG067923-01/AG/NIA NIH HHS/United States
GR  - NIH/NIEHS R01 ES027078/ES/NIEHS NIH HHS/United States
PT  - Journal Article
DEP - 20220215
PL  - Switzerland
TA  - Biology (Basel)
JT  - Biology
JID - 101587988
PMC - PMC8869661
OTO - NOTNLM
OT  - Alzheimer's disease
OT  - MCP-1
OT  - NF-kappaB
OT  - Z310
OT  - choroid plexus
OT  - lead
OT  - macrophage infiltration
OT  - p38 MAP kinase
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript; or in the decision to publish the results.
EDAT- 2022/02/26 06:00
MHDA- 2022/02/26 06:01
PMCR- 2022/02/15
CRDT- 2022/02/25 01:06
PHST- 2022/01/03 00:00 [received]
PHST- 2022/02/06 00:00 [revised]
PHST- 2022/02/10 00:00 [accepted]
PHST- 2022/02/25 01:06 [entrez]
PHST- 2022/02/26 06:00 [pubmed]
PHST- 2022/02/26 06:01 [medline]
PHST- 2022/02/15 00:00 [pmc-release]
AID - biology11020308 [pii]
AID - biology-11-00308 [pii]
AID - 10.3390/biology11020308 [doi]
PST - epublish
SO  - Biology (Basel). 2022 Feb 15;11(2):308. doi: 10.3390/biology11020308.