PMID- 35205803
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220301
IS  - 2072-6694 (Print)
IS  - 2072-6694 (Electronic)
IS  - 2072-6694 (Linking)
VI  - 14
IP  - 4
DP  - 2022 Feb 18
TI  - Novel Non-Invasive Quantification and Imaging of Eumelanin and DHICA Subunit in 
      Skin Lesions by Raman Spectroscopy and MCR Algorithm: Improving Dysplastic Nevi 
      Diagnosis.
LID - 10.3390/cancers14041056 [doi]
LID - 1056
AB  - Malignant melanoma (MM) is the most aggressive form of skin cancer, and around 
      30% of them may develop from pre-existing dysplastic nevi (DN). Diagnosis of DN 
      is a relevant clinical challenge, as these are intermediate lesions between 
      benign and malignant tumors, and, up to date, few studies have focused on their 
      diagnosis. In this study, the accuracy of Raman spectroscopy (RS) is assessed, 
      together with multivariate analysis (MA), to classify 44 biopsies of MM, DN and 
      compound nevus (CN) tumors. For this, we implement a novel methodology to 
      non-invasively quantify and localize the eumelanin pigment, considered as a 
      tumoral biomarker, by means of RS imaging coupled with the Multivariate Curve 
      Resolution-Alternative Least Squares (MCR-ALS) algorithm. This represents a step 
      forward with respect to the currently established technique for melanin analysis, 
      High-Performance Liquid Chromatography (HPLC), which is invasive and cannot 
      provide information about the spatial distribution of molecules. For the first 
      time, we show that the 5, 6-dihydroxyindole (DHI) to 
      5,6-dihydroxyindole-2-carboxylic acid (DHICA) ratio is higher in DN than in MM 
      and CN lesions. These differences in chemical composition are used by the Partial 
      Least Squares-Discriminant Analysis (PLS-DA) algorithm to identify DN lesions in 
      an efficient, non-invasive, fast, objective and cost-effective method, with 
      sensitivity and specificity of 100% and 94.1%, respectively.
FAU - Ruiz, Jose Javier
AU  - Ruiz JJ
AUID- ORCID: 0000-0001-8983-3612
AD  - ICFO-Institut de Ciencies Fotoniques, The Barcelona Institute of Science and 
      Technology, Castelldefels, 08860 Barcelona, Spain.
FAU - Marro, Monica
AU  - Marro M
AUID- ORCID: 0000-0003-4031-4605
AD  - ICFO-Institut de Ciencies Fotoniques, The Barcelona Institute of Science and 
      Technology, Castelldefels, 08860 Barcelona, Spain.
FAU - Galvan, Ismael
AU  - Galvan I
AUID- ORCID: 0000-0002-6523-8592
AD  - Department of Evolutionary Ecology, National Museum of Natural Sciences, CSIC, 
      28006 Madrid, Spain.
FAU - Bernabeu-Wittel, Jose
AU  - Bernabeu-Wittel J
AD  - Department of Dermatology, University Hospital Virgen del Rocio, 41013 Sevilla, 
      Spain.
FAU - Conejo-Mir, Julian
AU  - Conejo-Mir J
AD  - Department of Dermatology, University Hospital Virgen del Rocio, 41013 Sevilla, 
      Spain.
FAU - Zulueta-Dorado, Teresa
AU  - Zulueta-Dorado T
AD  - Department of Dermatology, University Hospital Virgen del Rocio, 41013 Sevilla, 
      Spain.
FAU - Guisado-Gil, Ana Belen
AU  - Guisado-Gil AB
AD  - Department of Dermatology, University Hospital Virgen del Rocio, 41013 Sevilla, 
      Spain.
FAU - Loza-Alvarez, Pablo
AU  - Loza-Alvarez P
AUID- ORCID: 0000-0002-3129-1213
AD  - ICFO-Institut de Ciencies Fotoniques, The Barcelona Institute of Science and 
      Technology, Castelldefels, 08860 Barcelona, Spain.
LA  - eng
PT  - Journal Article
DEP - 20220218
PL  - Switzerland
TA  - Cancers (Basel)
JT  - Cancers
JID - 101526829
PMC - PMC8870175
OTO - NOTNLM
OT  - Raman spectroscopy analysis
OT  - dysplastic nevus syndrome
OT  - eumelanin
OT  - melanoma
OT  - multivariate analysis
OT  - reactive oxygen species
OT  - skin neoplasms
COIS- The authors declare no conflict of interest.
EDAT- 2022/02/26 06:00
MHDA- 2022/02/26 06:01
PMCR- 2022/02/18
CRDT- 2022/02/25 01:07
PHST- 2021/12/09 00:00 [received]
PHST- 2022/02/11 00:00 [revised]
PHST- 2022/02/14 00:00 [accepted]
PHST- 2022/02/25 01:07 [entrez]
PHST- 2022/02/26 06:00 [pubmed]
PHST- 2022/02/26 06:01 [medline]
PHST- 2022/02/18 00:00 [pmc-release]
AID - cancers14041056 [pii]
AID - cancers-14-01056 [pii]
AID - 10.3390/cancers14041056 [doi]
PST - epublish
SO  - Cancers (Basel). 2022 Feb 18;14(4):1056. doi: 10.3390/cancers14041056.