PMID- 35207664
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220228
IS  - 2075-4426 (Print)
IS  - 2075-4426 (Electronic)
IS  - 2075-4426 (Linking)
VI  - 12
IP  - 2
DP  - 2022 Jan 28
TI  - Ultrastructural Changes in Mitochondria in Patients with Dilated Cardiomyopathy 
      and Parvovirus B19 Detected in Heart Tissue without Myocarditis.
LID - 10.3390/jpm12020177 [doi]
LID - 177
AB  - Understanding the meaning of parvovirus B19 (PB19V) in an etiology of dilated 
      cardiomyopathy (DCM) is difficult. Viruses change the dynamics of the 
      mitochondria by interfering with the mitochondrial process/function, causing the 
      alteration of mitochondrial morphology. In this study, the ultrastructural 
      changes in the mitochondria in endomyocardial biopsy (EMB) samples from patients 
      with DCM and PB19V were determined. METHODS: The PB19V evaluation was performed 
      in EMB specimens by real-time PCR in 20 patients (age: 28 +/- 6 years). The biopsy 
      specimens were examined by histo- and immunohistochemistry to detect the 
      inflammatory response. The ultrastructural features of the mitochondria were 
      evaluated by electron microscopy. RESULTS: The presence of PB19V in the heart 
      tissue without the presence of inflammatory process, defined according to Dallas 
      and immunohistochemical criteria, was associated with ultrastructural changes in 
      the mitochondria. Distinctive ultrastructural pathologies were indicated, such as 
      the presence of mitochondria in the vicinity of the expanded sarcoplasmic 
      reticulum with amorphous material, blurred structure of mitochondria, interrupted 
      outer mitochondrial membrane and mitophagy. CONCLUSIONS: Extending diagnostics 
      with ultrastructural analysis of biopsy samples provides new knowledge of the 
      changes associated with the presence of PB19V in the heart tissue. The observed 
      changes can be a basis for searching for the damage mechanisms, as well as for 
      new therapeutic solutions.
FAU - Pawlak, Agnieszka
AU  - Pawlak A
AD  - Department of Invasive Cardiology, Central Clinical Hospital of the Ministry of 
      Interior and Administration in Warsaw, 02-507 Warsaw, Poland.
AD  - Department of Applied Physiology, Mossakowski Medical Research Institute, Polish 
      Academy of Sciences, 02-106 Warsaw, Poland.
FAU - Gewartowska, Magdalena
AU  - Gewartowska M
AUID- ORCID: 0000-0002-7409-1799
AD  - Electron Microscopy Research Unit, Mossakowski Medical Research Institute, Polish 
      Academy of Sciences, 02-106 Warsaw, Poland.
FAU - Przybylski, Maciej
AU  - Przybylski M
AUID- ORCID: 0000-0002-0868-0018
AD  - Department of Medical Microbiology, Medical University of Warsaw, 02-004 Warsaw, 
      Poland.
FAU - Kuffner, Mateusz
AU  - Kuffner M
AUID- ORCID: 0000-0002-4726-0657
AD  - Electron Microscopy Research Unit, Mossakowski Medical Research Institute, Polish 
      Academy of Sciences, 02-106 Warsaw, Poland.
AD  - Division of Cardiology, Health Care Facility of the Ministry of Interior and 
      Administration in Rzeszow, 35-111 Rzeszow, Poland.
FAU - Wiligorska, Diana
AU  - Wiligorska D
AD  - Department of Invasive Cardiology, Central Clinical Hospital of the Ministry of 
      Interior and Administration in Warsaw, 02-507 Warsaw, Poland.
FAU - Gil, Robert
AU  - Gil R
AUID- ORCID: 0000-0002-9041-3313
AD  - Department of Invasive Cardiology, Central Clinical Hospital of the Ministry of 
      Interior and Administration in Warsaw, 02-507 Warsaw, Poland.
FAU - Krol, Zbigniew
AU  - Krol Z
AD  - Central Clinical Hospital of the Ministry of the Interior and Administration in 
      Warsaw, 02-507 Warsaw, Poland.
FAU - Frontczak-Baniewicz, Malgorzata
AU  - Frontczak-Baniewicz M
AD  - Electron Microscopy Research Unit, Mossakowski Medical Research Institute, Polish 
      Academy of Sciences, 02-106 Warsaw, Poland.
LA  - eng
PT  - Journal Article
DEP - 20220128
PL  - Switzerland
TA  - J Pers Med
JT  - Journal of personalized medicine
JID - 101602269
PMC - PMC8880015
OTO - NOTNLM
OT  - dilated cardiomyopathy
OT  - electron microscopy
OT  - mitochondria
OT  - parvovirus B19
OT  - viral infection
COIS- The authors declare no conflict of interest.
EDAT- 2022/02/26 06:00
MHDA- 2022/02/26 06:01
PMCR- 2022/01/28
CRDT- 2022/02/25 01:12
PHST- 2021/12/09 00:00 [received]
PHST- 2022/01/12 00:00 [revised]
PHST- 2022/01/20 00:00 [accepted]
PHST- 2022/02/25 01:12 [entrez]
PHST- 2022/02/26 06:00 [pubmed]
PHST- 2022/02/26 06:01 [medline]
PHST- 2022/01/28 00:00 [pmc-release]
AID - jpm12020177 [pii]
AID - jpm-12-00177 [pii]
AID - 10.3390/jpm12020177 [doi]
PST - epublish
SO  - J Pers Med. 2022 Jan 28;12(2):177. doi: 10.3390/jpm12020177.