PMID- 35207688
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220301
IS  - 2075-4426 (Print)
IS  - 2075-4426 (Electronic)
IS  - 2075-4426 (Linking)
VI  - 12
IP  - 2
DP  - 2022 Feb 1
TI  - Differential Expression of RAD51AP1 in Ovarian Cancer: Effects of siRNA In Vitro.
LID - 10.3390/jpm12020201 [doi]
LID - 201
AB  - BACKGROUND: DNA double strand breaks can affect genome integrity potentially 
      leading to cancer. RAD51-associated protein 1 (RAD51AP1), an accessory protein to 
      RAD51, is critical for homologous recombination, a key DNA damage response 
      pathway. Emerging studies indicate a novel role for RAD51AP1 in carcinogenesis. 
      Here we provide additional insight into the role of RAD51AP1 in ovarian cancer 
      (OvCa). METHODS: Gene expression and patient phenotype data were obtained from 
      TCGA and GTEX project consortia for bioinformatics analysis. Immunohistochemistry 
      of OvCa tissue microarray was undertaken. Functional analyses were performed in a 
      SKOV3 OvCa cell line with down-regulation of RAD51AP1 using siRNA. RESULTS: 
      RAD51AP1 is overexpressed at gene level in primary and recurrent OvCa compared to 
      controls. At protein level, RAD51AP1 was up-regulated in low grade serous tumors 
      compared to high grade OvCa. There was higher expression of RAD51AP1 in OvCa 
      metastatic to lymph nodes compared to primary cancer samples. Gene enrichment 
      analyses identified 12 differentially expressed genes (DEGs) related to OvCa, 
      eight of which are also common in tissue from patients with type 2 diabetes 
      mellitus (T2DM). CONCLUSIONS: RAD51AP1 is overexpressed in OvCa, Given the link 
      between OvCa and T2DM, the eight-gene signature shows potential for predictive 
      value.
FAU - Filipe, Alice
AU  - Filipe A
AD  - Department of Life Sciences, Division of Biosciences, College of Health, Medicine 
      and Life Sciences, Brunel University London, Uxbridge UB8 3PH, UK.
FAU - Katopodis, Periklis
AU  - Katopodis P
AUID- ORCID: 0000-0002-8892-2768
AD  - Department of Life Sciences, Division of Biosciences, College of Health, Medicine 
      and Life Sciences, Brunel University London, Uxbridge UB8 3PH, UK.
AD  - Division of Thoracic Surgery, The Royal Brompton & Harefield NHS Foundation 
      Trust, Harefield Hospital, Harefield UB9 6JH, UK.
FAU - Chudasama, Dimple
AU  - Chudasama D
AD  - Department of Life Sciences, Division of Biosciences, College of Health, Medicine 
      and Life Sciences, Brunel University London, Uxbridge UB8 3PH, UK.
FAU - Kerslake, Rachel
AU  - Kerslake R
AD  - Department of Life Sciences, Division of Biosciences, College of Health, Medicine 
      and Life Sciences, Brunel University London, Uxbridge UB8 3PH, UK.
AD  - Division of Thoracic Surgery, The Royal Brompton & Harefield NHS Foundation 
      Trust, Harefield Hospital, Harefield UB9 6JH, UK.
FAU - Jeyaneethi, Jeyarooban
AU  - Jeyaneethi J
AD  - Department of Life Sciences, Division of Biosciences, College of Health, Medicine 
      and Life Sciences, Brunel University London, Uxbridge UB8 3PH, UK.
AD  - Division of Thoracic Surgery, The Royal Brompton & Harefield NHS Foundation 
      Trust, Harefield Hospital, Harefield UB9 6JH, UK.
FAU - Anikin, Vladimir
AU  - Anikin V
AD  - Division of Thoracic Surgery, The Royal Brompton & Harefield NHS Foundation 
      Trust, Harefield Hospital, Harefield UB9 6JH, UK.
AD  - Department of Oncology and Reconstructive Surgery, Sechenov First Moscow State, 
      Medical University, 119146 Moscow, Russia.
FAU - Silva, Elisabete
AU  - Silva E
AD  - Department of Life Sciences, Division of Biosciences, College of Health, Medicine 
      and Life Sciences, Brunel University London, Uxbridge UB8 3PH, UK.
FAU - Kyrou, Ioannis
AU  - Kyrou I
AD  - Warwickshire Institute for the Study of Diabetes, Endocrinology and Metabolism 
      (WISDEM), University Hospitals Coventry and Warwickshire NHS Trust, Coventry CV2 
      2DX, UK.
AD  - Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK.
AD  - Centre for Sport, Exercise and Life Sciences, Research Institute for Health & 
      Wellbeing, Coventry University, Coventry CV1 5FB, UK.
AD  - Aston Medical Research Institute, Aston Medical School, College of Health and 
      Life Sciences, Aston University, Birmingham B4 7ET, UK.
AD  - Department of Food Science & Human Nutrition, School of Food and Nutritional 
      Sciences, Agricultural University of Athens, 11855 Athens, Greece.
FAU - Randeva, Harpal S
AU  - Randeva HS
AD  - Warwickshire Institute for the Study of Diabetes, Endocrinology and Metabolism 
      (WISDEM), University Hospitals Coventry and Warwickshire NHS Trust, Coventry CV2 
      2DX, UK.
AD  - Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK.
FAU - Sisu, Cristina
AU  - Sisu C
AUID- ORCID: 0000-0001-9371-0797
AD  - Department of Life Sciences, Division of Biosciences, College of Health, Medicine 
      and Life Sciences, Brunel University London, Uxbridge UB8 3PH, UK.
FAU - Hall, Marcia
AU  - Hall M
AUID- ORCID: 0000-0003-0039-5041
AD  - Department of Life Sciences, Division of Biosciences, College of Health, Medicine 
      and Life Sciences, Brunel University London, Uxbridge UB8 3PH, UK.
AD  - Mount Vernon Cancer Centre, Northwood, London HA6 2RN, UK.
FAU - Karteris, Emmanouil
AU  - Karteris E
AD  - Department of Life Sciences, Division of Biosciences, College of Health, Medicine 
      and Life Sciences, Brunel University London, Uxbridge UB8 3PH, UK.
AD  - Division of Thoracic Surgery, The Royal Brompton & Harefield NHS Foundation 
      Trust, Harefield Hospital, Harefield UB9 6JH, UK.
LA  - eng
GR  - 11016122/Royal Brompton & Harefield Hospitals Charity/
GR  - 11742100/Cancer Treatment & Research Trust (CTRT)/
PT  - Journal Article
DEP - 20220201
PL  - Switzerland
TA  - J Pers Med
JT  - Journal of personalized medicine
JID - 101602269
PMC - PMC8876735
OTO - NOTNLM
OT  - RAD51AP1
OT  - T2DM
OT  - biomarker
OT  - ovarian cancer
COIS- The authors declare no conflict of interest.
EDAT- 2022/02/26 06:00
MHDA- 2022/02/26 06:01
PMCR- 2022/02/01
CRDT- 2022/02/25 01:12
PHST- 2021/11/13 00:00 [received]
PHST- 2022/01/08 00:00 [revised]
PHST- 2022/01/25 00:00 [accepted]
PHST- 2022/02/25 01:12 [entrez]
PHST- 2022/02/26 06:00 [pubmed]
PHST- 2022/02/26 06:01 [medline]
PHST- 2022/02/01 00:00 [pmc-release]
AID - jpm12020201 [pii]
AID - jpm-12-00201 [pii]
AID - 10.3390/jpm12020201 [doi]
PST - epublish
SO  - J Pers Med. 2022 Feb 1;12(2):201. doi: 10.3390/jpm12020201.