PMID- 35212988
OWN - NLM
STAT- MEDLINE
DCOM- 20220301
LR  - 20220615
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 2455
DP  - 2022
TI  - Mouse Bone Marrow Cell Isolation and Macrophage Differentiation.
PG  - 85-91
LID - 10.1007/978-1-0716-2128-8_8 [doi]
AB  - The rapid increase in the incidence of obesity contributes to a parallel increase 
      in nonalcoholic steatohepatitis (NASH). Monocyte-derived macrophages, recruited 
      from the bone marrow to the liver, promote NASH-related inflammation and 
      fibrosis. In addition, adipose tissue macrophages (ATMs) release pro-inflammatory 
      cytokines (PICs) which stimulate adipose tissue lipolysis liberating free fatty 
      acids (FFAs) that can accumulate in the liver as triglycerides (TGs), thereby 
      inducing steatosis. As such, bone marrow-derived macrophages (BMDMs) function as 
      an essential tool to study the pathogenesis of NASH. BMDMs are primary bone 
      marrow-derived cells which are differentiated into macrophages in vitro in the 
      presence of growth factors. Macrophage colony-stimulating factor (M-CSF) is 
      required for the proliferation and differentiation of committed myeloid 
      progenitors into cells of the macrophage/monocyte lineage. Here, we describe a 
      protocol for the isolation of mouse bone marrow cells and subsequent macrophage 
      differentiation in which bone marrow cells are cultured in the presence of M-CSF, 
      supplemented either by conditioned medium from L929 cells or in purified form. 
      The efficiency of the differentiation is confirmed by immunofluorescent staining 
      of macrophage surface antigen F4/80. The BMDMs serve as an excellent ex vivo 
      model for a variety of studies, including hepatocyte-macrophage and 
      adipocyte-macrophage cross-talk regulating NASH.
CI  - (c) 2022. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Mendoza, Rachel
AU  - Mendoza R
AD  - Department of Human and Molecular Genetics, Virginia Commonwealth University, 
      Richmond, VA, USA.
FAU - Banerjee, Indranil
AU  - Banerjee I
AD  - Department of Human and Molecular Genetics, Virginia Commonwealth University, 
      Richmond, VA, USA.
FAU - Manna, Debashri
AU  - Manna D
AD  - Department of Human and Molecular Genetics, Virginia Commonwealth University, 
      Richmond, VA, USA.
FAU - Reghupaty, Saranya Chidambaranathan
AU  - Reghupaty SC
AD  - C. Kenneth and Dianne Wright Center for Clinical and Translational Research, 
      Virginia Commonwealth University, Richmond, VA, USA.
FAU - Yetirajam, Rajesh
AU  - Yetirajam R
AD  - Department of Human and Molecular Genetics, Virginia Commonwealth University, 
      Richmond, VA, USA.
FAU - Sarkar, Devanand
AU  - Sarkar D
AUID- ORCID: 0000-0003-0973-2323
AD  - Department of Human and Molecular Genetics, Virginia Commonwealth University, 
      Richmond, VA, USA. devanand.sarkar@vcuhealth.org.
AD  - Massey Cancer Center, Virginia Commonwealth University, Richmond, VA, USA. 
      devanand.sarkar@vcuhealth.org.
AD  - VCU Institute of Molecular Medicine, Virginia Commonwealth University, Richmond, 
      VA, USA. devanand.sarkar@vcuhealth.org.
LA  - eng
GR  - R01 CA230561/CA/NCI NIH HHS/United States
GR  - R01 CA240004/CA/NCI NIH HHS/United States
GR  - R01 CA244993/CA/NCI NIH HHS/United States
GR  - R01 DK107451/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - Cell Differentiation/physiology
MH  - Cells, Cultured
MH  - *Hematopoiesis
MH  - *Macrophage Colony-Stimulating Factor/metabolism/pharmacology
MH  - Macrophages/metabolism
MH  - Mice
MH  - Monocytes
PMC - PMC8936184
MID - NIHMS1785081
OTO - NOTNLM
OT  - Bone marrow cell
OT  - F4/80
OT  - L929 cells
OT  - Macrophage
OT  - Macrophage colony-stimulating factor (M-CSF)
OT  - Nonalcoholic steatohepatitis
EDAT- 2022/02/26 06:00
MHDA- 2022/03/03 06:00
PMCR- 2022/03/21
CRDT- 2022/02/25 12:24
PHST- 2022/02/25 12:24 [entrez]
PHST- 2022/02/26 06:00 [pubmed]
PHST- 2022/03/03 06:00 [medline]
PHST- 2022/03/21 00:00 [pmc-release]
AID - 10.1007/978-1-0716-2128-8_8 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2022;2455:85-91. doi: 10.1007/978-1-0716-2128-8_8.