PMID- 35213001
OWN - NLM
STAT- MEDLINE
DCOM- 20220301
LR  - 20220615
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2455
DP  - 2022
TI  - In Vivo Analysis of Necrosis and Ferroptosis in Nonalcoholic Steatohepatitis 
      (NASH).
PG  - 267-278
LID - 10.1007/978-1-0716-2128-8_21 [doi]
AB  - Nonalcoholic steatohepatitis (NASH) is a metabolic liver disease that progresses 
      from simple steatosis to the disease states such as chronic inflammation and 
      fibrosis. In most liver diseases, immunological responses caused by tissue 
      damages or viral infection contribute to the pathological advances, and various 
      types of cell death have been reported to be implicated in their pathogenesis. 
      However, the conventional detection of necrosis in vivo is not currently 
      available, whereas the detection method for apoptosis has been relatively 
      well-established. We recently reported a method for the in vivo detection of 
      necrotic cells in liver disease models by an intravenous injection of Propidium 
      Iodide (PI) into mice. We also provide standard methods for the evaluation of 
      lipid accumulation and fibrosis characteristic of NASH. In addition, by utilizing 
      these procedures and a murine model of steatohepatitis, we showed that 
      ferroptosis, a type of regulated necrotic cell death, could be involved in the 
      pathogenesis of NASH. These approaches allow us to explore the pathophysiological 
      roles of cell death in liver diseases.
CI  - (c) 2022. The Author(s), under exclusive license to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Tsurusaki, Shinya
AU  - Tsurusaki S
AD  - Department of Regenerative Medicine, Research Institute, National Center for 
      Global Health and Medicine, Tokyo, Japan.
FAU - Kanegae, Kazuko
AU  - Kanegae K
AD  - Department of Regenerative Medicine, Research Institute, National Center for 
      Global Health and Medicine, Tokyo, Japan.
FAU - Tanaka, Minoru
AU  - Tanaka M
AD  - Department of Regenerative Medicine, Research Institute, National Center for 
      Global Health and Medicine, Tokyo, Japan. m-tanaka@ri.ncgm.go.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - *Ferroptosis
MH  - Liver/metabolism
MH  - Liver Cirrhosis/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Necrosis/pathology
MH  - *Non-alcoholic Fatty Liver Disease/pathology
OTO - NOTNLM
OT  - Apoptosis
OT  - Carbon tetrachloride
OT  - Choline-deficient ethionine-supplemented diet
OT  - Ferroptosis
OT  - Hepatocyte
OT  - Necrosis
OT  - Nonalcoholic steatohepatitis
OT  - Programmed cell death
OT  - Propidium iodide
EDAT- 2022/02/26 06:00
MHDA- 2022/03/03 06:00
CRDT- 2022/02/25 12:24
PHST- 2022/02/25 12:24 [entrez]
PHST- 2022/02/26 06:00 [pubmed]
PHST- 2022/03/03 06:00 [medline]
AID - 10.1007/978-1-0716-2128-8_21 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2022;2455:267-278. doi: 10.1007/978-1-0716-2128-8_21.