PMID- 35213072
OWN - NLM
STAT- MEDLINE
DCOM- 20220301
LR  - 20220301
IS  - 2691-1299 (Electronic)
IS  - 2691-1299 (Linking)
VI  - 2
IP  - 2
DP  - 2022 Feb
TI  - How to Obtain High-Quality Metaphase Spreads for Molecular Cytogenetics.
PG  - e392
LID - 10.1002/cpz1.392 [doi]
AB  - Interphase or metaphase nuclei can be accessed in molecular cytogenetic analyses. 
      Metaphase spreads are routinely studied by fluorescence in situ hybridization 
      (FISH) to answer clinical genetic questions. Even though metaphase quality is 
      essential for FISH studies, there is limited ability in clinical cases to improve 
      the quality of cytogenetic preparations. However, the quality of preps is 
      important for the exact localization of FISH signals, which is necessary to 
      identify individual chromosomes and chromosomal sub-regions using inverted DAPI 
      banding. Here we present an efficient and easy-to-perform variant of standard 
      slide pretreatment before a normal FISH procedure. This method reproducibly leads 
      to solid, "steel," nonfuzzy, and well-DAPI-banded metaphases. This protocol works 
      in blood lymphocyte and amniotic fluid-derived fibroblasts. (c) 2022 The Authors. 
      Current Protocols published by Wiley Periodicals LLC. Basic Protocol: Slide 
      pretreatment for high-quality metaphases for molecular cytogenetics.
CI  - (c) 2022 The Authors. Current Protocols published by Wiley Periodicals LLC.
FAU - Liehr, Thomas
AU  - Liehr T
AD  - Jena University Hospital, Friedrich Schiller University, Institute of Human 
      Genetics, Jena, Germany.
FAU - Weise, Anja
AU  - Weise A
AD  - Jena University Hospital, Friedrich Schiller University, Institute of Human 
      Genetics, Jena, Germany.
FAU - Person, Luisa
AU  - Person L
AD  - Jena University Hospital, Friedrich Schiller University, Institute of Human 
      Genetics, Jena, Germany.
FAU - Padutsch, Niklas
AU  - Padutsch N
AD  - Jena University Hospital, Friedrich Schiller University, Institute of Human 
      Genetics, Jena, Germany.
FAU - Kankel, Stefanie
AU  - Kankel S
AD  - Jena University Hospital, Friedrich Schiller University, Institute of Human 
      Genetics, Jena, Germany.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Curr Protoc
JT  - Current protocols
JID - 101773894
SB  - IM
MH  - Cytogenetic Analysis
MH  - Cytogenetics
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Interphase
MH  - *Metaphase/genetics
OTO - NOTNLM
OT  - DAPI-banding
OT  - chromosome identification
OT  - fluorescence in situ hybridization
OT  - inverted DAPI
OT  - lymphocytes/amniocytes
OT  - metaphase FISH
OT  - molecular cytogenetics
EDAT- 2022/02/26 06:00
MHDA- 2022/03/03 06:00
CRDT- 2022/02/25 12:25
PHST- 2022/02/25 12:25 [entrez]
PHST- 2022/02/26 06:00 [pubmed]
PHST- 2022/03/03 06:00 [medline]
AID - 10.1002/cpz1.392 [doi]
PST - ppublish
SO  - Curr Protoc. 2022 Feb;2(2):e392. doi: 10.1002/cpz1.392.