PMID- 35213577
OWN - NLM
STAT- MEDLINE
DCOM- 20220309
LR  - 20220309
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 17
IP  - 2
DP  - 2022
TI  - Circadian rhythms modulate the effect of eccentric exercise on rat soleus 
      muscles.
PG  - e0264171
LID - 10.1371/journal.pone.0264171 [doi]
LID - e0264171
AB  - We investigated whether time-of-day dependent changes in the rat soleus (SOL) 
      muscle size, after eccentric exercises, operate via the mechanistic target of 
      rapamycin (mTOR) signaling pathway. For our first experiment, we assigned 
      9-week-old male Wistar rats randomly into four groups: light phase (zeitgeber 
      time; ZT6) non-trained control, dark phase (ZT18) non-trained control, light 
      phase-trained, and dark phase-trained. Trained animals performed 90 min of 
      downhill running once every 3 d for 8 weeks. The second experiment involved 
      dividing 9-week-old male Wistar rats to control and exercise groups. The latter 
      were subjected to 15 min of downhill running at ZT6 and ZT18. The absolute 
      (+12.8%) and relative (+9.4%) SOL muscle weights were higher in the light 
      phase-trained group. p70S6K phosphorylation ratio was 42.6% higher in the SOL 
      muscle of rats that had exercised only in light (non-trained ZT6). Collectively, 
      the degree of muscle hypertrophy in SOL is time-of-day dependent, perhaps via the 
      mTOR/p70S6K signaling.
FAU - Chang, Shuo-Wen
AU  - Chang SW
AD  - Graduate School of Health and Sports Science, Juntendo University, Chiba, Japan.
AD  - Department of Physical Education, National University of Tainan, Tainan, Taiwan.
FAU - Yoshihara, Toshinori
AU  - Yoshihara T
AUID- ORCID: 0000-0002-8465-5439
AD  - Graduate School of Health and Sports Science, Juntendo University, Chiba, Japan.
FAU - Tsuzuki, Takamasa
AU  - Tsuzuki T
AD  - Graduate School of Health and Sports Science, Juntendo University, Chiba, Japan.
AD  - Faculty of Pharmacy, Meijo University, Nagoya, Aichi, Japan.
FAU - Natsume, Toshiharu
AU  - Natsume T
AD  - Graduate School of Health and Sports Science, Juntendo University, Chiba, Japan.
AD  - School of Medicine, Tokai University, Hiratsuka, Kanagawa, Japan.
FAU - Kakigi, Ryo
AU  - Kakigi R
AD  - Faculty of Management & Information Sciences, Josai International University, 
      Chiba, Japan.
FAU - Machida, Shuichi
AU  - Machida S
AD  - Graduate School of Health and Sports Science, Juntendo University, Chiba, Japan.
FAU - Naito, Hisashi
AU  - Naito H
AUID- ORCID: 0000-0002-0522-9512
AD  - Graduate School of Health and Sports Science, Juntendo University, Chiba, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220225
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - *Circadian Rhythm
MH  - Male
MH  - Muscle, Skeletal/*metabolism
MH  - *Physical Conditioning, Animal
MH  - Rats
MH  - Rats, Wistar
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
MH  - *Running
MH  - *Signal Transduction
MH  - TOR Serine-Threonine Kinases/metabolism
PMC - PMC8880858
COIS- The authors have declared that no competing interests exist.
EDAT- 2022/02/26 06:00
MHDA- 2022/03/11 06:00
PMCR- 2022/02/25
CRDT- 2022/02/25 17:15
PHST- 2021/03/06 00:00 [received]
PHST- 2022/02/04 00:00 [accepted]
PHST- 2022/02/25 17:15 [entrez]
PHST- 2022/02/26 06:00 [pubmed]
PHST- 2022/03/11 06:00 [medline]
PHST- 2022/02/25 00:00 [pmc-release]
AID - PONE-D-21-07409 [pii]
AID - 10.1371/journal.pone.0264171 [doi]
PST - epublish
SO  - PLoS One. 2022 Feb 25;17(2):e0264171. doi: 10.1371/journal.pone.0264171. 
      eCollection 2022.