PMID- 35213700
OWN - NLM
STAT- MEDLINE
DCOM- 20220412
LR  - 20230226
IS  - 1525-3163 (Electronic)
IS  - 0021-8812 (Print)
IS  - 0021-8812 (Linking)
VI  - 100
IP  - 4
DP  - 2022 Apr 1
TI  - Zearalenone promotes follicle development through activating the SIRT1/PGC-1alpha 
      signaling pathway in the ovaries of weaned gilts.
LID - 10.1093/jas/skac058 [doi]
LID - skac058
AB  - This study aimed to investigate the effect of zearalenone (ZEA) exposure on 
      follicular development in weaned gilts, and its mechanism based on the silent 
      information regulator 1 (SIRT1)/peroxisome proliferator-activated receptor-gamma 
      co-activator 1alpha (PGC-1alpha) signaling pathway. A total of 32 healthy female weaned 
      piglets (Landrace x Yorkshire x Duroc) with an average body weight of 
      12.39 +/- 0.24 kg were randomly allotted to a basal diet supplemented with 0, 0.15, 
      1.5, or 3.0 mg/kg ZEA for a 32-d feeding test. Blood and ovarian samples were 
      obtained at the end of the experiment to determine serum toxin concentrations, 
      ovarian histology, and the expressions of proliferating cell nuclear antigen 
      (PCNA) and SIRT1/PGC-1alpha signaling pathway-related genes. Results showed that the 
      vulva area, serum concentrations of ZEA, alpha-zearalenol and beta-zearalenol, the 
      thickness of the growing follicular layer, and the diameter of the largest 
      growing follicles, as well as the expressions of SIRT1, PGC-1alpha, estrogen-related 
      receptor alpha (ERRalpha), ATP synthase subunit beta (ATP5B), and PCNA, increased 
      linearly (P < 0.05) with increasing dietary ZEA, whereas the thickness of the 
      primordial follicle layer decreased linearly (P < 0.05). Immunohistochemical 
      analysis showed that the immunoreactive substances of SIRT1 and PGC-1alpha in the 
      ovaries enhanced with the increasing dietary ZEA (P < 0.05). In addition, the 
      thickness of the growing follicular layer and the diameter of the largest growing 
      follicle were positively correlated with relative mRNA and protein expressions of 
      SIRT1, PGC-1alpha, ERRalpha, ATP5B, and PCNA (P < 0.05). However, the thickness of the 
      primordial follicle layer was negatively correlated with the mRNA and protein 
      expression of SIRT1, PGC-1alpha, ERRalpha, ATP5B, and PCNA (P < 0.05). Interestingly, the 
      1.5 mg/kg ZEA treatment had highly hyperplastic follicles, whereas 3.0 mg/kg ZEA 
      resulted in a large number of follicular atresia, which indicated that low-dose 
      ZEA exposure accelerated follicular proliferation, while high-dose ZEA promoted 
      follicular atresia, although the critical value interval needs further 
      confirmation. Results provide a theoretical basis for finding the therapeutic 
      target of ZEA-induced reproductive disorders in weaned gilts.
CI  - (c) The Author(s) 2022. Published by Oxford University Press on behalf of the 
      American Society of Animal Science. All rights reserved. 
For permissions, please 
      e-mail: journals.permissions@oup.com.
FAU - Wan, Boyang
AU  - Wan B
AD  - Department of Animal Sciences and Technology and Shandong Provincial Key 
      Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong 
      Agricultural University, Taian, Shandong 271018, China.
FAU - Huang, Libo
AU  - Huang L
AUID- ORCID: 0000-0002-2318-1954
AD  - Department of Animal Sciences and Technology and Shandong Provincial Key 
      Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong 
      Agricultural University, Taian, Shandong 271018, China.
FAU - Jing, Changwei
AU  - Jing C
AD  - Technical Department, Shandong Chinwhiz Co., Weifang, Shandong 262400, China.
FAU - Li, Yang
AU  - Li Y
AD  - Department of Animal Sciences and Technology and Shandong Provincial Key 
      Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong 
      Agricultural University, Taian, Shandong 271018, China.
FAU - Jiao, Ning
AU  - Jiao N
AD  - Department of Animal Sciences and Technology and Shandong Provincial Key 
      Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong 
      Agricultural University, Taian, Shandong 271018, China.
FAU - Liang, Ming
AU  - Liang M
AD  - Department of Feeding Microecology, Shandong Baolaililai Bioengineering Co., 
      Ltd., Taian, Shandong 271001, China.
FAU - Jiang, Shuzhen
AU  - Jiang S
AD  - Department of Animal Sciences and Technology and Shandong Provincial Key 
      Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong 
      Agricultural University, Taian, Shandong 271018, China.
FAU - Yang, Weiren
AU  - Yang W
AD  - Department of Animal Sciences and Technology and Shandong Provincial Key 
      Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong 
      Agricultural University, Taian, Shandong 271018, China.
LA  - eng
GR  - ZR2021MC048/Natural Science Foundation of Shandong Province/
GR  - SDAIT-08-04/Shandong Province Pig Industry Technology System/
GR  - 2019JZZY020609/Major Innovative Projects of Shandong Province/
PT  - Journal Article
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (RNA, Messenger)
RN  - 5W827M159J (Zearalenone)
RN  - EC 3.5.1.- (Sirtuin 1)
SB  - IM
MH  - Animals
MH  - Female
MH  - Follicular Atresia
MH  - Ovary/metabolism
MH  - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha/genetics
MH  - Proliferating Cell Nuclear Antigen/metabolism
MH  - RNA, Messenger/metabolism
MH  - Signal Transduction
MH  - Sirtuin 1/genetics/metabolism/pharmacology
MH  - Sus scrofa/genetics
MH  - Swine
MH  - *Zearalenone/pharmacology
PMC - PMC9030242
OAB - Zearalenone (ZEA), an estrogenic fusariotoxin, existing in various grains and 
      feedstuffs, could disrupt the endocrine and reproductive systems. However, the 
      underlying mechanisms of ZEA-induced follicular development have not been fully 
      elucidated. This study was to explore the effects and the possible molecular 
      mechanisms of ZEA on follicular development. A total of 32 female weaned piglets 
      were randomly allotted to a basal diet supplemented with 0, 0.15, 1.5, or 
      3.0 mg/kg ZEA for a 32-d feeding test. The results showed that dietary ZEA 
      increased the vulva area and serum toxin levels, and accelerated follicle 
      development. Moreover, 1.5 and 3.0 mg/kg ZEA changed the expression of 
      proliferating cell nuclear antigen (PCNA) and activated the silent information 
      regulator 1 (SIRT1)/peroxisome proliferator-activated receptor-gamma co-activator 1alpha 
      (PGC-1alpha) signaling pathway. Meanwhile, ZEA could promote follicle development by 
      regulating PCNA expression through activation of the SIRT1/PGC-1alpha signaling 
      pathway. The very significant contribution was that the proliferated follicles 
      significantly increased with the increasing ZEA concentration when the ZEA in the 
      diet was less than 1.5 mg/kg; however, atretic follicles significantly increased 
      when the ZEA in the diet was 3.0 mg/kg. This study provides a theoretical basis 
      for finding the therapeutic target of ZEA-induced reproductive disorders in 
      weaned gilts.
OABL- eng
OTO - NOTNLM
OT  - SIRT1/PGC-1alpha pathway
OT  - follicle development
OT  - gilt
OT  - ovary
OT  - zearalenone
EDAT- 2022/02/26 06:00
MHDA- 2022/04/13 06:00
PMCR- 2023/02/25
CRDT- 2022/02/25 17:16
PHST- 2022/01/07 00:00 [received]
PHST- 2022/02/24 00:00 [accepted]
PHST- 2022/02/26 06:00 [pubmed]
PHST- 2022/04/13 06:00 [medline]
PHST- 2022/02/25 17:16 [entrez]
PHST- 2023/02/25 00:00 [pmc-release]
AID - 6537148 [pii]
AID - skac058 [pii]
AID - 10.1093/jas/skac058 [doi]
PST - ppublish
SO  - J Anim Sci. 2022 Apr 1;100(4):skac058. doi: 10.1093/jas/skac058.