PMID- 35214198
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220301
IS  - 1999-4923 (Print)
IS  - 1999-4923 (Electronic)
IS  - 1999-4923 (Linking)
VI  - 14
IP  - 2
DP  - 2022 Feb 21
TI  - A Non-thermal Biocompatible Plasma-Modified Chitosan Scaffold Enhances Osteogenic 
      Differentiation in Bone Marrow Stem Cells.
LID - 10.3390/pharmaceutics14020465 [doi]
LID - 465
AB  - Non-thermal biocompatible plasma (NBP) was considered as an efficient tool in 
      tissue engineering to modify the surface of biomaterials. Three-dimensional 
      chitosan scaffolds have been extensively used in different ways because it holds 
      some remarkable properties, including biodegradability and biocompatibility. In 
      this study, we evaluated the osteogenic potential of NBP-treated chitosan 
      scaffolds using two different plasma sources: a dielectric barrier discharge 
      (NBP-DBD) and a soft jet (NBP-J). The surface modification of the scaffold was 
      evaluated using scanning electron microscopy. For osteogenic differentiation of 
      cells, proliferation and differentiation were tested by using bone marrow-derived 
      stem cells (BMSCs). We observed that cell viability using NBP-DBD and NBP-J 
      treated chitosan scaffolds yielded significant improvements in cell viability and 
      differentiation. The results obtained with MTT and live/dead assays showed that 
      NBP-modified scaffold increases cell metabolic by MTT assay and live/dead assay. 
      It also observed that the NBP treatment is more effective at 5 min with DBD and 
      was selected for further investigations. Enhanced osteogenic differentiation was 
      observed using NBP-treated scaffolds, as reflected by increased alkaline 
      phosphatase activity. Our findings showed that NBP is an innovative and 
      beneficial tool for modifying chitosan scaffolds to increase their activity, 
      making them suitable as biocompatible materials and for bone tissue engineering.
FAU - Han, Ihn
AU  - Han I
AUID- ORCID: 0000-0002-1372-7319
AD  - Department of Plasma Bio Display, Kwangwoon University, Seoul 01897, Korea.
AD  - Plasma Bioscience Research Center, Kwangwoon University, Seoul 01897, Korea.
FAU - Rana, Juie Nahushkumar
AU  - Rana JN
AUID- ORCID: 0000-0002-9982-4098
AD  - Department of Plasma Bio Display, Kwangwoon University, Seoul 01897, Korea.
AD  - Plasma Bioscience Research Center, Kwangwoon University, Seoul 01897, Korea.
FAU - Kim, Ji-Hye
AU  - Kim JH
AD  - Ellitech Medical Incorporation, Seoul 02584, Korea.
FAU - Choi, Eun Ha
AU  - Choi EH
AUID- ORCID: 0000-0001-5385-1878
AD  - Plasma Bioscience Research Center, Kwangwoon University, Seoul 01897, Korea.
FAU - Kim, Youngsun
AU  - Kim Y
AUID- ORCID: 0000-0002-1725-0968
AD  - Department of Obstetrics and Gynecology, Kyung Hee University Medical Center, 
      Seoul 02447, Korea.
LA  - eng
GR  - 2020R1I1A1A01073071/Ministry of Education/
GR  - 2021R1A6A1A03038785/Ministry of Education/
PT  - Journal Article
DEP - 20220221
PL  - Switzerland
TA  - Pharmaceutics
JT  - Pharmaceutics
JID - 101534003
PMC - PMC8874420
OTO - NOTNLM
OT  - biomaterial
OT  - chitosan scaffolds
OT  - non-thermal plasma
OT  - osteogenic differentiation
OT  - tissue engineering
COIS- J.-H.K. is employee of Ellitech Medical Incorporation. Ellitech Medical 
      Incorporation had no role in the design of the study; in the collection, 
      analyses, or interpretation of data; in the writing of the manuscript, or in the 
      decision to publish the results.
EDAT- 2022/02/27 06:00
MHDA- 2022/02/27 06:01
PMCR- 2022/02/21
CRDT- 2022/02/26 01:02
PHST- 2022/01/20 00:00 [received]
PHST- 2022/02/09 00:00 [revised]
PHST- 2022/02/16 00:00 [accepted]
PHST- 2022/02/26 01:02 [entrez]
PHST- 2022/02/27 06:00 [pubmed]
PHST- 2022/02/27 06:01 [medline]
PHST- 2022/02/21 00:00 [pmc-release]
AID - pharmaceutics14020465 [pii]
AID - pharmaceutics-14-00465 [pii]
AID - 10.3390/pharmaceutics14020465 [doi]
PST - epublish
SO  - Pharmaceutics. 2022 Feb 21;14(2):465. doi: 10.3390/pharmaceutics14020465.