PMID- 35214663
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240324
IS  - 2076-393X (Print)
IS  - 2076-393X (Electronic)
IS  - 2076-393X (Linking)
VI  - 10
IP  - 2
DP  - 2022 Jan 27
TI  - Prevalence of HPV Infection and p16(INK4a) Overexpression in Surgically Treated 
      Laryngeal Squamous Cell Carcinoma.
LID - 10.3390/vaccines10020204 [doi]
LID - 204
AB  - Background: The mucosal high-risk (HR) human papillomavirus (HPV) is associated 
      with oropharyngeal carcinogenesis. Aims of this study were to evaluate the 
      prevalence of HR-HPV infection in laryngeal squamous cell carcinoma (LSCC) from 
      different subsites, and the clinico-biological meaning of p16 overexpression. 
      Methods: Ninety-seven LSCCs submitted to primary surgery (n = 75) or to 
      post-irradiation salvage laryngectomy (n = 22) were evaluated for HR-HPV DNA and 
      RNA using Luminex-based assays. p16 immunohistochemistry was performed. Results: 
      HR-HPV DNA from HPV16 was detected in seven cases (8.75%), without significant 
      differences between supraglottic and glottic lesions. HPV RNA was never detected. 
      p16 overexpression correlated with HR-HPV DNA, but the kappa agreement score was 
      poor. HPV DNA showed no impact on prognosis. p16 overexpression was associated 
      with a better survival (OS, RFS) in primarily operated cases, while an inverse 
      association with OS was observed in the salvage surgery group. Conclusions: 
      HR-HPV infection appears to have a marginal role in LSCC independent of the 
      anatomical subsite. p16 expression is deregulated in LSCC independent of HPV but 
      displays a prognostic role in patients submitted to primary surgery. The negative 
      predictive role of p16 overexpression in patients undergoing salvage surgery 
      deserves more investigations for validation and elucidation of its clinical 
      relevance.
FAU - Gallus, Roberto
AU  - Gallus R
AD  - Otolaryngology, Mater Olbia Hospital, 07026 Olbia, Italy.
FAU - Gheit, Tarik
AU  - Gheit T
AD  - Infections and Cancer Biology Group, International Agency for Research on Cancer, 
      World Health Organization, 69008 Lyon, France.
FAU - Holzinger, Dana
AU  - Holzinger D
AD  - German Cancer Research Center (DKFZ), Division of Molecular Diagnostics of 
      Oncogenic Infections, 69120 Heidelberg, Germany.
FAU - Petrillo, Marco
AU  - Petrillo M
AUID- ORCID: 0000-0003-0306-4328
AD  - Gynecologic and Obstetric Unit, Department of Medical, Surgical and Experimental 
      Sciences, University of Sassari, 07100 Sassari, Italy.
FAU - Rizzo, Davide
AU  - Rizzo D
AUID- ORCID: 0000-0003-1830-3731
AD  - Otolaryngology Division, Azienda Ospedaliero Universitaria di Sassari, 07100 
      Sassari, Italy.
AD  - Dipartimento delle Scienze Mediche, Chirurgiche e Sperimentali, Universita di 
      Sassari, 07100 Sassari, Italy.
FAU - Petrone, Gianluigi
AU  - Petrone G
AD  - Department of Women and Child Health and Public Health, Pathology Area, 
      Fondazione Policlinico Universitario A. Gemelli-IRCCS, 00168 Rome, Italy.
FAU - Micciche, Francesco
AU  - Micciche F
AD  - Dipartimento di Scienze Radiologiche, Radioterapiche ed Ematologiche, UOC di 
      Radioterapia, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, 
      Italy.
FAU - Mattiucci, Gian Carlo
AU  - Mattiucci GC
AD  - Dipartimento di Scienze Radiologiche, Radioterapiche ed Ematologiche, UOC di 
      Radioterapia, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, 
      Italy.
AD  - Radiation Oncology Unit, Mater Olbia Hospital, 07026 Olbia, Italy.
FAU - Arciuolo, Damiano
AU  - Arciuolo D
AD  - Department of Women and Child Health and Public Health, Pathology Area, 
      Fondazione Policlinico Universitario A. Gemelli-IRCCS, 00168 Rome, Italy.
FAU - Capobianco, Giampiero
AU  - Capobianco G
AUID- ORCID: 0000-0002-5523-8943
AD  - Gynecologic and Obstetric Unit, Department of Medical, Surgical and Experimental 
      Sciences, University of Sassari, 07100 Sassari, Italy.
FAU - Delogu, Giovanni
AU  - Delogu G
AD  - Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e 
      Perioperatorie, Universita Cattolica del Sacro Cuore, 00168 Roma, Italy.
AD  - Mater Olbia Hospital, 07026 Olbia, Italy.
FAU - Valentini, Vincenzo
AU  - Valentini V
AD  - Dipartimento di Scienze Radiologiche, Radioterapiche ed Ematologiche, UOC di 
      Radioterapia, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, 
      Italy.
FAU - Tommasino, Massimo
AU  - Tommasino M
AD  - Infections and Cancer Biology Group, International Agency for Research on Cancer, 
      World Health Organization, 69008 Lyon, France.
FAU - Bussu, Francesco
AU  - Bussu F
AUID- ORCID: 0000-0001-6261-2772
AD  - Otolaryngology Division, Azienda Ospedaliero Universitaria di Sassari, 07100 
      Sassari, Italy.
AD  - Dipartimento delle Scienze Mediche, Chirurgiche e Sperimentali, Universita di 
      Sassari, 07100 Sassari, Italy.
LA  - eng
GR  - 001/WHO_/World Health Organization/International
PT  - Journal Article
DEP - 20220127
PL  - Switzerland
TA  - Vaccines (Basel)
JT  - Vaccines
JID - 101629355
PMC - PMC8874711
OTO - NOTNLM
OT  - biomarkers
OT  - cyclin-dependent kinase inhibitor p16
OT  - human papilloma virus
OT  - laryngeal neoplasms
COIS- The authors declare no conflict of interest.
EDAT- 2022/02/27 06:00
MHDA- 2022/02/27 06:01
PMCR- 2022/01/27
CRDT- 2022/02/26 01:03
PHST- 2021/12/19 00:00 [received]
PHST- 2022/01/23 00:00 [revised]
PHST- 2022/01/24 00:00 [accepted]
PHST- 2022/02/26 01:03 [entrez]
PHST- 2022/02/27 06:00 [pubmed]
PHST- 2022/02/27 06:01 [medline]
PHST- 2022/01/27 00:00 [pmc-release]
AID - vaccines10020204 [pii]
AID - vaccines-10-00204 [pii]
AID - 10.3390/vaccines10020204 [doi]
PST - epublish
SO  - Vaccines (Basel). 2022 Jan 27;10(2):204. doi: 10.3390/vaccines10020204.