PMID- 35214815
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220301
IS  - 2223-7747 (Print)
IS  - 2223-7747 (Electronic)
IS  - 2223-7747 (Linking)
VI  - 11
IP  - 4
DP  - 2022 Feb 11
TI  - Advances in the Molecular Cytogenetics of Bananas, Family Musaceae.
LID - 10.3390/plants11040482 [doi]
LID - 482
AB  - The banana is a staple food crop and represents an important trade commodity for 
      millions of people living in tropical and subtropical countries. The most 
      important edible banana clones originated from natural crosses between diploid 
      Musa balbisiana and various subspecies of M. acuminata. It is worth mentioning 
      that evolution and speciation in the Musaceae family were accompanied by 
      large-scale chromosome structural changes, indicating possible reasons for lower 
      fertility or complete sterility of these vegetatively propagated clones. 
      Chromosomal changes, often accompanied by changes in genome size, are one of the 
      driving forces underlying speciation in plants. They can clarify the genomic 
      constitution of edible bananas and shed light on their origin and on 
      diversification processes in members of the Musaceae family. This article reviews 
      the development of molecular cytogenetic approaches, ranging from classical 
      fluorescence in situ hybridization (FISH) using common cytogenetic markers to 
      oligo painting FISH. We discuss differences in genome size and chromosome number 
      across the Musaceae family in addition to the development of new 
      chromosome-specific cytogenetic probes and their use in genome structure and 
      comparative karyotype analysis. The impact of these methodological advances on 
      our knowledge of Musa genome evolution at the chromosomal level is demonstrated. 
      In addition to citing published results, we include our own new unpublished 
      results and outline future applications of molecular cytogenetics in banana 
      research.
FAU - Simonikova, Denisa
AU  - Simonikova D
AUID- ORCID: 0000-0002-1066-0577
AD  - Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the 
      Region Hana for Biotechnological and Agricultural Research, 77900 Olomouc, Czech 
      Republic.
FAU - Cizkova, Jana
AU  - Cizkova J
AUID- ORCID: 0000-0001-7787-1849
AD  - Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the 
      Region Hana for Biotechnological and Agricultural Research, 77900 Olomouc, Czech 
      Republic.
FAU - Zoulova, Veronika
AU  - Zoulova V
AD  - Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the 
      Region Hana for Biotechnological and Agricultural Research, 77900 Olomouc, Czech 
      Republic.
AD  - Department of Cell Biology and Genetics, Faculty of Science, Palacky University, 
      77900 Olomouc, Czech Republic.
FAU - Christelova, Pavla
AU  - Christelova P
AD  - Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the 
      Region Hana for Biotechnological and Agricultural Research, 77900 Olomouc, Czech 
      Republic.
FAU - Hribova, Eva
AU  - Hribova E
AUID- ORCID: 0000-0002-6868-4344
AD  - Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the 
      Region Hana for Biotechnological and Agricultural Research, 77900 Olomouc, Czech 
      Republic.
LA  - eng
GR  - 19-20303S/Czech Science Foundation/
GR  - LTT19009/Ministry of Education, Youth, and Sports of the Czech Republic/
PT  - Journal Article
PT  - Review
DEP - 20220211
PL  - Switzerland
TA  - Plants (Basel)
JT  - Plants (Basel, Switzerland)
JID - 101596181
PMC - PMC8879896
OTO - NOTNLM
OT  - BAC clones
OT  - DNA repeats
OT  - chromosomes
OT  - flow cytometry
OT  - fluorescence in situ hybridization
OT  - karyotyping
OT  - oligo painting
OT  - rRNA genes
COIS- All authors declare no conflict of interest.
EDAT- 2022/02/27 06:00
MHDA- 2022/02/27 06:01
PMCR- 2022/02/11
CRDT- 2022/02/26 01:04
PHST- 2021/12/23 00:00 [received]
PHST- 2022/02/02 00:00 [revised]
PHST- 2022/02/07 00:00 [accepted]
PHST- 2022/02/26 01:04 [entrez]
PHST- 2022/02/27 06:00 [pubmed]
PHST- 2022/02/27 06:01 [medline]
PHST- 2022/02/11 00:00 [pmc-release]
AID - plants11040482 [pii]
AID - plants-11-00482 [pii]
AID - 10.3390/plants11040482 [doi]
PST - epublish
SO  - Plants (Basel). 2022 Feb 11;11(4):482. doi: 10.3390/plants11040482.