PMID- 35216141
OWN - NLM
STAT- MEDLINE
DCOM- 20220315
LR  - 20220315
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 4
DP  - 2022 Feb 11
TI  - Immunohistochemical Expression Pattern of FGFR1, FGFR2, RIP5, and HIP2 in 
      Developing and Postnatal Kidneys of Dab1(-/-) (yotari) Mice.
LID - 10.3390/ijms23042025 [doi]
LID - 2025
AB  - This study aimed to explore how Dab1 gene functional silencing influences the 
      spatial and temporal expression patterns of fibroblast growth factor receptor 1 
      (FGFR1), fibroblast growth factor receptor 2 (FGFR2), receptor-interacting 
      protein kinase 5 (RIP5), and huntingtin-interacting protein 2 (HIP2) in the 
      developing and postnatal kidneys of the yotari mice as potential determinants of 
      normal kidney formation and function. Dab1(-/-) animal kidneys exhibit diminished 
      FGFR1/FGFR2 expression in all examined developmental stages, whereas RIP5 cell 
      immunoreactivity demonstrated negligible variation. The HIP2 expression revealed 
      a discernible difference during the postnatal period, where we noted a 
      significant decrease in almost all the observed kidney structures of yotari 
      animals. An extracellular signal-regulated kinase (Erk1/2) and mammalian target 
      of rapamycin (mTOR) expression in yotari kidneys decreased in embryonic and 
      postnatal developmental phases for which we can hypothesize that the Erk1/2 
      signaling pathway in the yotari mice kidneys is dependent on Reelin with Dab1 
      only partially implicated in Reelin-mediated MEK/Erk1/2 activation. The 
      impairment of FGFR1 and FGFR2 expression suggests the involvement of the observed 
      markers in generating the CAKUT phenotype resulting in renal hypoplasia. Our 
      study demonstrates the critical role of HIP2 in reducing cell death throughout 
      nephrogenesis and maturation in wild-type mice and indicates a possible 
      connection between decreased HIP2 expression in postnatal kidney structures and 
      observed podocyte injury in yotari. Our results emphasize the crucial function of 
      the examined markers throughout normal kidney development and their potential 
      participation in kidney pathology and diagnostics, where they might serve as 
      biomarkers and therapeutic targets.
FAU - Kelam, Nela
AU  - Kelam N
AUID- ORCID: 0000-0002-6529-5474
AD  - Department of Anatomy, Histology and Embryology, University of Split School of 
      Medicine, Soltanska 2, 21000 Split, Croatia.
FAU - Racetin, Anita
AU  - Racetin A
AD  - Department of Anatomy, Histology and Embryology, University of Split School of 
      Medicine, Soltanska 2, 21000 Split, Croatia.
AD  - Department of Medical Genetics, University of Mostar School of Medicine, 88000 
      Mostar, Bosnia and Herzegovina.
FAU - Katsuyama, Yu
AU  - Katsuyama Y
AD  - Department of Anatomy, Shiga University of Medical Science, Otsu 520-2192, Japan.
FAU - Vukojevic, Katarina
AU  - Vukojevic K
AUID- ORCID: 0000-0003-2182-2890
AD  - Department of Anatomy, Histology and Embryology, University of Split School of 
      Medicine, Soltanska 2, 21000 Split, Croatia.
AD  - Department of Medical Genetics, University of Mostar School of Medicine, 88000 
      Mostar, Bosnia and Herzegovina.
FAU - Kostic, Sandra
AU  - Kostic S
AD  - Department of Anatomy, Histology and Embryology, University of Split School of 
      Medicine, Soltanska 2, 21000 Split, Croatia.
LA  - eng
GR  - IP-06-2016-2575/Croatian Science Foundation/
PT  - Journal Article
DEP - 20220211
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Biomarkers)
RN  - 0 (Dab1 protein, mouse)
RN  - 0 (Nerve Tissue Proteins)
RN  - EC 2.3.2.23 (Ube2k protein, mouse)
RN  - EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes)
RN  - EC 2.7.10.1 (Fgfr1 protein, mouse)
RN  - EC 2.7.10.1 (Fgfr2 protein, mouse)
RN  - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 1)
RN  - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 2)
RN  - EC 2.7.11.1 (Receptor-Interacting Protein Serine-Threonine Kinases)
RN  - Cakut
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Kidney/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nerve Tissue Proteins/*metabolism
MH  - Phenotype
MH  - Receptor, Fibroblast Growth Factor, Type 1/*metabolism
MH  - Receptor, Fibroblast Growth Factor, Type 2/*metabolism
MH  - Receptor-Interacting Protein Serine-Threonine Kinases/*metabolism
MH  - Signal Transduction/physiology
MH  - Ubiquitin-Conjugating Enzymes/*metabolism
MH  - Urogenital Abnormalities/metabolism
MH  - Vesico-Ureteral Reflux/metabolism
PMC - PMC8879463
OTO - NOTNLM
OT  - Erk1/2
OT  - FGFR1
OT  - FGFR2
OT  - HIP2
OT  - RIP5
OT  - kidney development
OT  - mTOR
OT  - yotari mice
COIS- The authors declare no conflict of interest.
EDAT- 2022/02/27 06:00
MHDA- 2022/03/16 06:00
PMCR- 2022/02/11
CRDT- 2022/02/26 01:07
PHST- 2021/12/23 00:00 [received]
PHST- 2022/02/04 00:00 [revised]
PHST- 2022/02/10 00:00 [accepted]
PHST- 2022/02/26 01:07 [entrez]
PHST- 2022/02/27 06:00 [pubmed]
PHST- 2022/03/16 06:00 [medline]
PHST- 2022/02/11 00:00 [pmc-release]
AID - ijms23042025 [pii]
AID - ijms-23-02025 [pii]
AID - 10.3390/ijms23042025 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Feb 11;23(4):2025. doi: 10.3390/ijms23042025.