PMID- 35216228
OWN - NLM
STAT- MEDLINE
DCOM- 20220314
LR  - 20220314
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 4
DP  - 2022 Feb 14
TI  - An Interleukin-4 and Interleukin-13 Induced Atopic Dermatitis Human Skin 
      Equivalent Model by a Skin-On-A-Chip.
LID - 10.3390/ijms23042116 [doi]
LID - 2116
AB  - Currently, the mechanism of progression of atopic dermatitis (AD) is not well 
      understood because there is no physiologically appropriate disease model in terms 
      of disease complexity and multifactoriality. Type 2 inflammation, mediated by 
      interleukin (IL)-4 and IL-13, plays an important role in AD. In this study, 
      full-thickness human skin equivalents consisting of human-derived cells were 
      fabricated from pumpless microfluidic chips and stimulated with IL-4 and IL-13. 
      The morphological properties, gene expression, cytokine secretion and protein 
      expression of the stimulated human skin equivalent (HSE) epidermis were 
      investigated. The results showed epidermal and spongy formations similar to those 
      observed in lesions in AD, and decreased expression of barrier-related filaggrin, 
      loricrin and involucrin genes and proteins induced by IL-4Ralpha signaling. In 
      addition, we induced the expression of carbonic anhydrase II (CAII), a gene 
      specifically expressed in the epidermis of patients with AD. Thus, AD human skin 
      equivalents can be used to mimic the key pathological features of atopic 
      dermatitis, overcoming the limitations of existing studies that rely solely on 
      mouse models and have been unable to translate their effects to humans. Our 
      results will be useful for future research on the development of therapeutic 
      agents for atopic dermatitis.
FAU - Kim, Kyunghee
AU  - Kim K
AD  - Interdisciplinary Program of Nano-Medical Device Engineering, Hallym University, 
      Chuncheon 24252, Korea.
AD  - Major in Materials Science and Engineering, Hallym University, Chuncheon 24252, 
      Korea.
FAU - Kim, Hyeju
AU  - Kim H
AD  - Major in Materials Science and Engineering, Hallym University, Chuncheon 24252, 
      Korea.
FAU - Sung, Gun Yong
AU  - Sung GY
AUID- ORCID: 0000-0001-8515-4786
AD  - Interdisciplinary Program of Nano-Medical Device Engineering, Hallym University, 
      Chuncheon 24252, Korea.
AD  - Major in Materials Science and Engineering, Hallym University, Chuncheon 24252, 
      Korea.
AD  - Integrative Materials Research Institute, Hallym University, Chuncheon 24252, 
      Korea.
LA  - eng
GR  - NRF-2020R1A2C2009928/Ministry of Science ICT and Future Planning/
GR  - HRF-202112-001/Hallym University/
PT  - Journal Article
DEP - 20220214
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (IL13 protein, human)
RN  - 0 (IL4 protein, human)
RN  - 0 (Interleukin-13)
RN  - 0 (Membrane Proteins)
RN  - 0 (loricrin)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Animals
MH  - Dermatitis, Atopic/drug therapy/*metabolism
MH  - Eczema/drug therapy/metabolism/pathology
MH  - Epidermis/drug effects/metabolism/pathology
MH  - Gene Expression/drug effects/physiology
MH  - Humans
MH  - Inflammation/drug therapy/metabolism/pathology
MH  - Interleukin-13/*metabolism
MH  - Interleukin-4/*metabolism
MH  - Keratinocytes/drug effects/metabolism/pathology
MH  - Lab-On-A-Chip Devices
MH  - Membrane Proteins/pharmacology
MH  - Rats
MH  - Skin/drug effects/*metabolism/pathology
PMC - PMC8878506
OTO - NOTNLM
OT  - atopic dermatitis
OT  - carbonic anhydrase II
OT  - human skin equivalent
OT  - interleukin-13
OT  - interleukin-4
OT  - skin-on-a-chip
COIS- The authors declared no conflict of interest.
EDAT- 2022/02/27 06:00
MHDA- 2022/03/15 06:00
PMCR- 2022/02/14
CRDT- 2022/02/26 01:07
PHST- 2022/01/13 00:00 [received]
PHST- 2022/01/30 00:00 [revised]
PHST- 2022/02/08 00:00 [accepted]
PHST- 2022/02/26 01:07 [entrez]
PHST- 2022/02/27 06:00 [pubmed]
PHST- 2022/03/15 06:00 [medline]
PHST- 2022/02/14 00:00 [pmc-release]
AID - ijms23042116 [pii]
AID - ijms-23-02116 [pii]
AID - 10.3390/ijms23042116 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Feb 14;23(4):2116. doi: 10.3390/ijms23042116.