PMID- 35216236
OWN - NLM
STAT- MEDLINE
DCOM- 20220314
LR  - 20220314
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 4
DP  - 2022 Feb 14
TI  - Alteration of Ethanol Reward by Prior Mephedrone Exposure: The Role of Age and 
      Matrix Metalloproteinase-9 (MMP-9).
LID - 10.3390/ijms23042122 [doi]
LID - 2122
AB  - Mephedrone, a synthetic cathinone, is widely abused by adolescents and young 
      adults. The aim of this study was to determine: (i) whether prior mephedrone 
      exposure would alter ethanol reward and (ii) whether age and matrix 
      metalloproteinase-9 (MMP-9) are important in this regard. In our research, male 
      Wistar rats at postnatal day 30 (PND30) received mephedrone at the dose of 10 
      mg/kg, i.p., 3 times a day for 7 days. To clarify the role of MMP-9 in the 
      mephedrone effects, one mephedrone-treated group received minocycline, as an 
      MMP-9 antagonist. Animals were then assigned to conditioned place preference 
      (CPP) procedure at PND38 (adolescent) or at PND69 (adult). After the CPP test 
      (PND48/79), expression of dopamine D1 receptors (D1R), Cav1.2 (a subtype of 
      L-type calcium channels), and MMP-9 was quantified in the rat ventral striatum 
      (vSTR). The influence of mephedrone administration on the N-methyl-D-aspartate 
      glutamate receptors (NMDAR) subunits (GluN1, GluN2A, and GluN2B) was then 
      assessed in the vSTR of adult rats (only). These results indicate that, in 
      contrast with adolescent rats, adult rats with prior mephedrone administration 
      appear to be more sensitive to the ethanol effect in the CPP test under the 
      drug-free state. The mephedrone effect in adult rats was associated with 
      upregulation of D1R, NMDAR/GluN2B, MMP-9, and Cav1.2 signaling. MMP-9 appears to 
      contribute to these changes in proteins expression because minocycline 
      pretreatment blocked mephedrone-evoked sensitivity to ethanol reward. Thus, our 
      results suggest that prior mephedrone exposure differentially alters ethanol 
      reward in adolescent and adult rats.
FAU - Grochecki, Pawel
AU  - Grochecki P
AD  - Department of Pharmacology and Pharmacodynamics, Medical University, Chodzki 4a, 
      20-093 Lublin, Poland.
FAU - Smaga, Irena
AU  - Smaga I
AUID- ORCID: 0000-0002-7783-8181
AD  - Department of Drug Addiction Pharmacology, Maj Institute of Pharmacology, Polish 
      Academy of Sciences, Smetna 12, 31-343 Krakow, Poland.
FAU - Marszalek-Grabska, Marta
AU  - Marszalek-Grabska M
AUID- ORCID: 0000-0002-2487-9131
AD  - Department of Experimental and Clinical Pharmacology, Medical University, 
      Jaczewskiego 8b, 20-090 Lublin, Poland.
FAU - Lopatynska-Mazurek, Malgorzata
AU  - Lopatynska-Mazurek M
AUID- ORCID: 0000-0001-5266-1968
AD  - Department of Pharmacology and Pharmacodynamics, Medical University, Chodzki 4a, 
      20-093 Lublin, Poland.
FAU - Slowik, Tymoteusz
AU  - Slowik T
AD  - Experimental Medicine Center, Medical University of Lublin, Jaczewskiego 8, 
      20-090 Lublin, Poland.
FAU - Gibula-Tarlowska, Ewa
AU  - Gibula-Tarlowska E
AUID- ORCID: 0000-0001-7791-0633
AD  - Department of Pharmacology and Pharmacodynamics, Medical University, Chodzki 4a, 
      20-093 Lublin, Poland.
FAU - Kedzierska, Ewa
AU  - Kedzierska E
AD  - Department of Pharmacology and Pharmacodynamics, Medical University, Chodzki 4a, 
      20-093 Lublin, Poland.
FAU - Listos, Joanna
AU  - Listos J
AD  - Department of Pharmacology and Pharmacodynamics, Medical University, Chodzki 4a, 
      20-093 Lublin, Poland.
FAU - Filip, Malgorzata
AU  - Filip M
AUID- ORCID: 0000-0002-4321-7942
AD  - Department of Drug Addiction Pharmacology, Maj Institute of Pharmacology, Polish 
      Academy of Sciences, Smetna 12, 31-343 Krakow, Poland.
FAU - Kotlinska, Jolanta H
AU  - Kotlinska JH
AUID- ORCID: 0000-0002-4178-1515
AD  - Department of Pharmacology and Pharmacodynamics, Medical University, Chodzki 4a, 
      20-093 Lublin, Poland.
LA  - eng
GR  - Grant UMO No. 2017/25/B/NZ7/01845/National Science Centre (NCN)/
GR  - (DS 22/21)/Medical University of Lublin/
PT  - Journal Article
DEP - 20220214
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 3K9958V90M (Ethanol)
RN  - 44RAL3456C (Methamphetamine)
RN  - 8BA8T27317 (mephedrone)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - EC 3.4.24.35 (Mmp9 protein, rat)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Ethanol/*adverse effects
MH  - Male
MH  - Matrix Metalloproteinase 9/*metabolism
MH  - Methamphetamine/adverse effects/*analogs & derivatives
MH  - Rats
MH  - Rats, Wistar
MH  - Reward
MH  - Signal Transduction/drug effects
MH  - Ventral Striatum/drug effects/metabolism
PMC - PMC8877998
OTO - NOTNLM
OT  - MMP-9
OT  - age
OT  - conditioned place preference
OT  - ethanol
OT  - mephedrone
OT  - minocycline
COIS- The authors declare no conflict of interest.
EDAT- 2022/02/27 06:00
MHDA- 2022/03/15 06:00
PMCR- 2022/02/14
CRDT- 2022/02/26 01:07
PHST- 2022/01/20 00:00 [received]
PHST- 2022/02/08 00:00 [revised]
PHST- 2022/02/11 00:00 [accepted]
PHST- 2022/02/26 01:07 [entrez]
PHST- 2022/02/27 06:00 [pubmed]
PHST- 2022/03/15 06:00 [medline]
PHST- 2022/02/14 00:00 [pmc-release]
AID - ijms23042122 [pii]
AID - ijms-23-02122 [pii]
AID - 10.3390/ijms23042122 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Feb 14;23(4):2122. doi: 10.3390/ijms23042122.